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Q04279

- SEG1_YEAST

UniProt

Q04279 - SEG1_YEAST

Protein

Eisosome protein SEG1

Gene

SEG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Important for the biogenesis of eisosomes, large cytoplasmic protein assemblies that localize to specialized domains on the plasma membrane to cluster specific proteins at sites of membrane invaginations. Required for efficient incorporation of the eisosome component PIL1 into eisosomes.1 Publication

    GO - Biological processi

    1. eisosome assembly Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32786-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Eisosome protein SEG1
    Alternative name(s):
    Stability of eisosomes guaranteed protein 1
    Gene namesi
    Name:SEG1
    Ordered Locus Names:YMR086W
    ORF Names:YM9582.10
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR086w.
    SGDiS000004692. SEG1.

    Subcellular locationi

    Cell membrane 3 Publications; Peripheral membrane protein 3 Publications; Cytoplasmic side 3 Publications
    Note: Localizes to eisosomes.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. eisosome Source: SGD

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 960960Eisosome protein SEG1PRO_0000203284Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei48 – 481Phosphoserine1 Publication
    Modified residuei220 – 2201Phosphoserine1 Publication
    Modified residuei235 – 2351Phosphoserine1 Publication
    Modified residuei238 – 2381Phosphoserine1 Publication
    Modified residuei319 – 3191Phosphoserine1 Publication
    Modified residuei339 – 3391Phosphoserine1 Publication
    Modified residuei352 – 3521Phosphoserine3 Publications
    Modified residuei434 – 4341Phosphoserine1 Publication
    Modified residuei461 – 4611Phosphoserine2 Publications
    Modified residuei467 – 4671Phosphothreonine1 Publication
    Modified residuei630 – 6301Phosphoserine1 Publication
    Modified residuei675 – 6751Phosphothreonine2 Publications
    Modified residuei758 – 7581Phosphoserine1 Publication
    Modified residuei816 – 8161Phosphoserine3 Publications
    Modified residuei818 – 8181Phosphoserine3 Publications
    Modified residuei855 – 8551Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ04279.
    PaxDbiQ04279.
    PRIDEiQ04279.

    Expressioni

    Gene expression databases

    GenevestigatoriQ04279.

    Interactioni

    Subunit structurei

    Component of eisosomes, large cytoplasmic protein assemblies that localize to specialized domains termed MCCs on the plasma membrane.1 Publication

    Protein-protein interaction databases

    BioGridi35261. 37 interactions.
    DIPiDIP-6346N.
    IntActiQ04279. 3 interactions.
    MINTiMINT-2492235.
    STRINGi4932.YMR086W.

    Structurei

    3D structure databases

    ProteinModelPortaliQ04279.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi941 – 96020Lys-rich (basic, sufficient to bind lipids, important for targeting the protein to the plasma membrane in small buds)Add
    BLAST

    Sequence similaritiesi

    Belongs to the SEG1 family.Curated

    Phylogenomic databases

    eggNOGiNOG12793.
    GeneTreeiENSGT00530000067521.
    OMAiPNIFANI.
    OrthoDBiEOG7B05SF.

    Sequencei

    Sequence statusi: Complete.

    Q04279-1 [UniParc]FASTAAdd to Basket

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    MFRRRTTAPE MEQADPTAVA AAASIGKLLM KKGNQSDNEQ RPTYRSASMT    50
    NLRKPSAPKR MSSISSISSE SRRSDGKGRP GKINSLTQRS SMGKGDSLNS 100
    PLTKEPQHKT RSHNRTSSLP NQRGQQSRNS SGLQRQKSKT HQRISYDEAQ 150
    RTFKDFGGPQ ARGILTGQHR TENPSGSIPL RTTRKYIPGP NGLVAIEVPV 200
    EKPSNANTSK LLRRSNSAHS ALNARNGSLL RKKVSQESLH SQPKKTSSLG 250
    NTSSTQAKKG QKAVQERNLA KKHPINSNVP LIETQVREET DQELKLDNSN 300
    SSESETVVNS ENNLEKPSSL NIEKDDLSKL IHENIELESF IEEKGKEKPL 350
    NSDQEDVFIS EKTVKGDVQW PKNNRQASTL EKTFNYDNEE KREGNKPVAE 400
    HPLPEAEVDD KSVEQLNNIS SSGSYSAQGS VENSKPENGN KNLENDTTSS 450
    PTQDLDEKSR SIEEKDTLEG TESSKKVEKP DNCCEKISGA NTSSKKGNVG 500
    DTKDEFFDTV EESDQKTSKS NSSKNTDHSN QTEPTPSLAQ YLRTSNTYLS 550
    RKNQSKQAEQ EKFHKPEAPM VPVTKVVTPI KSALKKSSGS SNHDSSMYSD 600
    NSPANGAYLS LTTAENTRLN AQMTMSDSVS RRASLKRSSI KRPQSVGQFR 650
    SIRSNSPSPP EKINNKRHSA IPLGTPEKGK PKRNSVMASL SKNSQQIQEP 700
    ASVYESNGPN KPKNQINKNI KRGSQIAQNN KPSTKDMNSI LYPKEPPPRK 750
    SSFEKTRSNE SHLGFKKLSL RNGNFEEALS ESYNGQASQN STNVNRTDTA 800
    QEFFKYLGHS SRFADSDSED ESQFFNQGPS KYNTETEGNK TSGNKNSNGG 850
    NGAFSLFKSK SKQKENNVVS PGVSSPNHTT TDPAITSKKV DKKFSGLSLR 900
    AASEAEPAKN SNPSMTNRLR FSSNPENGES RLPQAQEVSV TKEKKGSFGK 950
    KLKKIFGRKK 960
    Length:960
    Mass (Da):105,874
    Last modified:November 1, 1997 - v1
    Checksum:i05A4FA27129DB09B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti167 – 1671G → E in AAT92595. (PubMed:17322287)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49259 Genomic DNA. Translation: CAA89232.1.
    AY692576 Genomic DNA. Translation: AAT92595.1.
    BK006946 Genomic DNA. Translation: DAA09983.1.
    PIRiS54461.
    RefSeqiNP_013803.1. NM_001182586.1.

    Genome annotation databases

    EnsemblFungiiYMR086W; YMR086W; YMR086W.
    GeneIDi855110.
    KEGGisce:YMR086W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49259 Genomic DNA. Translation: CAA89232.1 .
    AY692576 Genomic DNA. Translation: AAT92595.1 .
    BK006946 Genomic DNA. Translation: DAA09983.1 .
    PIRi S54461.
    RefSeqi NP_013803.1. NM_001182586.1.

    3D structure databases

    ProteinModelPortali Q04279.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35261. 37 interactions.
    DIPi DIP-6346N.
    IntActi Q04279. 3 interactions.
    MINTi MINT-2492235.
    STRINGi 4932.YMR086W.

    Proteomic databases

    MaxQBi Q04279.
    PaxDbi Q04279.
    PRIDEi Q04279.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR086W ; YMR086W ; YMR086W .
    GeneIDi 855110.
    KEGGi sce:YMR086W.

    Organism-specific databases

    CYGDi YMR086w.
    SGDi S000004692. SEG1.

    Phylogenomic databases

    eggNOGi NOG12793.
    GeneTreei ENSGT00530000067521.
    OMAi PNIFANI.
    OrthoDBi EOG7B05SF.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32786-MONOMER.

    Miscellaneous databases

    NextBioi 978446.

    Gene expression databases

    Genevestigatori Q04279.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: SUBCELLULAR LOCATION.
    5. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-352; SER-816 AND SER-818, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    6. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-48; SER-235; SER-238; THR-675 AND SER-758, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-352; SER-461; SER-816 AND SER-818, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Unifying fluorescence microscopy and mass spectrometry for studying protein complexes in cells."
      Deng C., Xiong X., Krutchinsky A.N.
      Mol. Cell. Proteomics 8:1413-1423(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-220; SER-319; SER-339; SER-352; SER-434; SER-461; THR-467; SER-630; THR-675; SER-816; SER-818 AND SER-855, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Membrane compartment occupied by Can1 (MCC) and eisosome subdomains of the fungal plasma membrane."
      Douglas L.M., Wang H.X., Li L., Konopka J.B.
      Membranes 1:394-411(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE NAME.
    11. Cited for: FUNCTION, SUBCELLULAR LOCATION, SUBUNIT.

    Entry informationi

    Entry nameiSEG1_YEAST
    AccessioniPrimary (citable) accession number: Q04279
    Secondary accession number(s): D6VZQ9, Q6B304
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3