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Protein

Vacuolar protein sorting-associated protein 20

Gene

VPS20

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Class E VPS protein implicated in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. The lumenal sequestrated membrane proteins will be targeted into the vacuole after fusion of the endosome with the vacuole. Acts a component of the ESCRT-III complex, which appears to be critical for late steps in MVB sorting, such as membrane invagination and final cargo sorting and recruitment of late-acting components of the sorting machinery. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complex assemblies. Required for the oligomerization of SNF7 into a membrane-associated filament. The VPS20-SNF7 subcomplex is responsible for the membrane association of the ESCRT-III complex. Also required for the RIM101 repressor proteolytic activation.5 Publications

GO - Biological processi

  • intralumenal vesicle formation Source: SGD
  • late endosome to vacuole transport Source: SGD
  • protein transport Source: UniProtKB-KW
  • ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32779-MONOMER.
ReactomeiR-SCE-1632852. Macroautophagy.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein sorting-associated protein 20
Alternative name(s):
Amino acid sensor-independent protein 10
Charged multivesicular body protein 6
ESCRT-III complex subunit VPS20
Vacuolar protein-targeting protein 20
Gene namesi
Name:VPS20
Synonyms:ASI10, CHM6, VPT20
Ordered Locus Names:YMR077C
ORF Names:YM9582.02C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR077C.
SGDiS000004682. VPS20.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • ESCRT III complex Source: SGD
  • vacuolar membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Membrane, Vacuole

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi2 – 21G → A: Loss of membrane association. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved
Chaini2 – 221220Vacuolar protein sorting-associated protein 20PRO_0000211515Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine1 Publication

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

MaxQBiQ04272.

PTM databases

iPTMnetiQ04272.

Interactioni

Subunit structurei

Core component of the ESCRT-III complex (endosomal sorting required for transport complex III). ESCRT-III appears to be sequentially assembled as a flat lattice on the endosome membrane and forms a transient 450 kDa complex that contains DID4, oligomerized SNF7, VPS20 and VPS24. SNF7 oligomerization into a membrane-associated filament is nucleated by association of SNF7 with VPS20; the process is terminated through association of VPS24, possibly by capping the SNF7 filament. VPS24 subsequently associates with DID4/VPS2. Interacts with the VPS4. Interacts with VPS25; the interaction mediates the association with the ESCRT-II complex.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SNF7P399294EBI-28157,EBI-17554
VPS25P471423EBI-28157,EBI-25595
VPS4P529176EBI-28157,EBI-20475

Protein-protein interaction databases

BioGridi35253. 108 interactions.
DIPiDIP-1961N.
IntActiQ04272. 9 interactions.
MINTiMINT-390066.

Structurei

3D structure databases

ProteinModelPortaliQ04272.
SMRiQ04272. Positions 119-169.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili72 – 178107Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the SNF7 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00720000108863.
HOGENOMiHOG000208642.
InParanoidiQ04272.
KOiK12195.
OMAiECLARDD.
OrthoDBiEOG7DRJF3.

Family and domain databases

InterProiIPR005024. Snf7_fam.
[Graphical view]
PfamiPF03357. Snf7. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q04272-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGQKSSKVHI TKTDRAILEV KRSKDEIHKF TRRTDNLILV EKSQLKDLIR
60 70 80 90 100
KNPENYKSNM KVRFLLKRIH YQEHLLQQAS DQLINLENMV STLEFKMVEK
110 120 130 140 150
QFINGLKNGN EILKKLNKEF SNVDELMDDV QDQIAYQNEI NETLSRSLVG
160 170 180 190 200
TSNYEDDLDK ELDALESELN PEKMNNAKVA NMPSTEGLPS LPQGEQTEQK
210 220
EREEFATEER SDTKEPLALL S
Length:221
Mass (Da):25,638
Last modified:January 23, 2007 - v3
Checksum:iCDC88BC4ADA3D602
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49259 Genomic DNA. Translation: CAA89223.1.
BK006946 Genomic DNA. Translation: DAA09975.1.
PIRiS54452.
RefSeqiNP_013794.1. NM_001182576.1.

Genome annotation databases

EnsemblFungiiYMR077C; YMR077C; YMR077C.
GeneIDi855101.
KEGGisce:YMR077C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49259 Genomic DNA. Translation: CAA89223.1.
BK006946 Genomic DNA. Translation: DAA09975.1.
PIRiS54452.
RefSeqiNP_013794.1. NM_001182576.1.

3D structure databases

ProteinModelPortaliQ04272.
SMRiQ04272. Positions 119-169.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35253. 108 interactions.
DIPiDIP-1961N.
IntActiQ04272. 9 interactions.
MINTiMINT-390066.

PTM databases

iPTMnetiQ04272.

Proteomic databases

MaxQBiQ04272.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR077C; YMR077C; YMR077C.
GeneIDi855101.
KEGGisce:YMR077C.

Organism-specific databases

EuPathDBiFungiDB:YMR077C.
SGDiS000004682. VPS20.

Phylogenomic databases

GeneTreeiENSGT00720000108863.
HOGENOMiHOG000208642.
InParanoidiQ04272.
KOiK12195.
OMAiECLARDD.
OrthoDBiEOG7DRJF3.

Enzyme and pathway databases

BioCyciYEAST:G3O-32779-MONOMER.
ReactomeiR-SCE-1632852. Macroautophagy.

Miscellaneous databases

NextBioi978422.
PROiQ04272.

Family and domain databases

InterProiIPR005024. Snf7_fam.
[Graphical view]
PfamiPF03357. Snf7. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "A role for Saccharomyces cerevisiae fatty acid activation protein 4 in regulating protein N-myristoylation during entry into stationary phase."
    Ashrafi K., Farazi T.A., Gordon J.I.
    J. Biol. Chem. 273:25864-25874(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: MYRISTOYLATION AT GLY-2.
  4. "CHMP1 functions as a member of a newly defined family of vesicle trafficking proteins."
    Howard T.L., Stauffer D.R., Degnin C.R., Hollenberg S.M.
    J. Cell Sci. 114:2395-2404(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "A family of small coiled-coil-forming proteins functioning at the late endosome in yeast."
    Kranz A., Kinner A., Koelling R.
    Mol. Biol. Cell 12:711-723(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  6. "Escrt-III: an endosome-associated heterooligomeric protein complex required for mvb sorting."
    Babst M., Katzmann D.J., Estepa-Sabal E.J., Meerloo T., Emr S.D.
    Dev. Cell 3:271-282(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN THE ESCRT-III COMPLEX, INTERACTION WITH VPS2; VPS24 AND SNF7, SUBCELLULAR LOCATION, MUTAGENESIS OF GLY-2.
  7. "Vps20p and Vta1p interact with Vps4p and function in multivesicular body sorting and endosomal transport in Saccharomyces cerevisiae."
    Yeo S.C.L., Xu L., Ren J., Boulton V.J., Wagle M.D., Liu C., Ren G., Wong P., Zahn R., Sasajala P., Yang H., Piper R.C., Munn A.L.
    J. Cell Sci. 116:3957-3970(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH VPS4.
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. "ESCRT-II, an endosome-associated complex required for protein sorting: crystal structure and interactions with ESCRT-III and membranes."
    Teo H., Perisic O., Gonzalez B., Williams R.L.
    Dev. Cell 7:559-569(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH VPS25.
  11. "Multivesicular body-ESCRT components function in pH response regulation in Saccharomyces cerevisiae and Candida albicans."
    Xu W., Smith F.J. Jr., Subaran R., Mitchell A.P.
    Mol. Biol. Cell 15:5528-5537(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "Structural characterization of the ATPase reaction cycle of endosomal AAA protein Vps4."
    Xiao J., Xia H., Yoshino-Koh K., Zhou J., Xu Z.
    J. Mol. Biol. 374:655-670(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH VPS4.
  13. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiVPS20_YEAST
AccessioniPrimary (citable) accession number: Q04272
Secondary accession number(s): D6VZQ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: May 11, 2016
This is version 130 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 937 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.