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Protein

Protein GIS4

Gene

GIS4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in the Ras/cAMP signaling pathway.1 Publication

GO - Biological processi

  • cellular ion homeostasis Source: SGD
  • intracellular signal transduction Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-32611-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein GIS4
Gene namesi
Name:GIS4
Ordered Locus Names:YML006C
ORF Names:YM9571.13C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XIII

Organism-specific databases

CYGDiYML006c.
EuPathDBiFungiDB:YML006C.
SGDiS000004465. GIS4.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 774774Protein GIS4PRO_0000087498Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei165 – 1651Phosphoserine1 Publication
Modified residuei304 – 3041Phosphoserine1 Publication
Modified residuei616 – 6161Phosphoserine1 Publication
Modified residuei622 – 6221Phosphoserine2 Publications
Modified residuei707 – 7071Phosphoserine1 Publication
Modified residuei720 – 7201Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ04233.
PaxDbiQ04233.

Expressioni

Gene expression databases

GenevestigatoriQ04233.

Interactioni

Protein-protein interaction databases

BioGridi35164. 62 interactions.
DIPiDIP-1334N.
IntActiQ04233. 12 interactions.
MINTiMINT-384235.
STRINGi4932.YML006C.

Structurei

3D structure databases

ProteinModelPortaliQ04233.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi147 – 1526Poly-Asn
Compositional biasi183 – 20321Thr-richAdd
BLAST
Compositional biasi329 – 35830Ser-richAdd
BLAST
Compositional biasi340 – 34910Poly-Ser
Compositional biasi353 – 3586Poly-Ser
Compositional biasi576 – 58914Asp-richAdd
BLAST

Phylogenomic databases

eggNOGiNOG45211.
HOGENOMiHOG000000749.
InParanoidiQ04233.
OMAiKILFYTM.
OrthoDBiEOG72ZCPP.

Sequencei

Sequence statusi: Complete.

Q04233-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQKSVRVGDY FDNDDNGLWS WYLTNLRLGD FEELIGNQLK YTLLKRFLNS
60 70 80 90 100
HFYGDNNISA RPNKKILLVS IPENVHEDIS ILEIFLKDYF HLEKLEHIQI
110 120 130 140 150
SKLTHSHCYN HENHYLLTDN LNNFQDPTFL EFASTSWQVQ KNSKALNNNN
160 170 180 190 200
RNSIPPPTIS SSKASNGKLE SNVSDDQWSN INTQTSTATR TNTNTRTLTS
210 220 230 240 250
PDTVDINVTS VNSQSNNNDT PQDNENEVDE EDATSSIVLN FSHSRTVDSK
260 270 280 290 300
PNRLPKIFPS YTNEDYTPSH SEIMSIDSFA GEDVSSTYPG QDLSLTTARR
310 320 330 340 350
EDESGQDEVE DHYSRVSHDL GDESIDQASY SMESSVSYTS YSSSSNSSSA
360 370 380 390 400
HYSLSSSSRG NPKRENIDHT NATYVSELSS ITSSIDNLTT STTPEEEDNL
410 420 430 440 450
IHHNYDAQGY GSGEDDGEEV YDDEDLSSSD YSVLSILPSI SICDSLGYFR
460 470 480 490 500
LVLQSILIQD PDTKEIFTAI RQSNNKPTMA SVTDDWLLYD SNFSMNNLQI
510 520 530 540 550
LTLQDLLDIK RSFPKILFYT MVIVTNSGKQ VEEEFKNPNY DNREGISKEQ
560 570 580 590 600
PLDSELSLTN DPQQYFPTAY NNGYNDYIDD EDDEDDGDDA SLSEQSGPQM
610 620 630 640 650
YIPTRMESNV TTAHRSIRTV NSIGEWAFNR HNSVTKIDKS NSNELDNSKT
660 670 680 690 700
GESTVLSSEP HPMTQLSNSN TTSSNFSHSL KTKNSHKPNS KGNNESNSKN
710 720 730 740 750
ELKKIKSSIN AMSAVERSKS LPLPTLLKSL SGIDNPTHAT NKDRKRWKFQ
760 770
MNRFKNHKNS GSAGTDKSQR CAIM
Length:774
Mass (Da):87,140
Last modified:November 1, 1997 - v1
Checksum:iC7E837AA7001E241
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49810 Genomic DNA. Translation: CAA89946.1.
BK006946 Genomic DNA. Translation: DAA09893.1.
PIRiS55113.
RefSeqiNP_013708.1. NM_001182361.1.

Genome annotation databases

EnsemblFungiiYML006C; YML006C; YML006C.
GeneIDi855006.
KEGGisce:YML006C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49810 Genomic DNA. Translation: CAA89946.1.
BK006946 Genomic DNA. Translation: DAA09893.1.
PIRiS55113.
RefSeqiNP_013708.1. NM_001182361.1.

3D structure databases

ProteinModelPortaliQ04233.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35164. 62 interactions.
DIPiDIP-1334N.
IntActiQ04233. 12 interactions.
MINTiMINT-384235.
STRINGi4932.YML006C.

Proteomic databases

MaxQBiQ04233.
PaxDbiQ04233.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYML006C; YML006C; YML006C.
GeneIDi855006.
KEGGisce:YML006C.

Organism-specific databases

CYGDiYML006c.
EuPathDBiFungiDB:YML006C.
SGDiS000004465. GIS4.

Phylogenomic databases

eggNOGiNOG45211.
HOGENOMiHOG000000749.
InParanoidiQ04233.
OMAiKILFYTM.
OrthoDBiEOG72ZCPP.

Enzyme and pathway databases

BioCyciYEAST:G3O-32611-MONOMER.

Miscellaneous databases

NextBioi978166.
PROiQ04233.

Gene expression databases

GenevestigatoriQ04233.

Family and domain databases

ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Yeast genes GIS1-4: multicopy suppressors of the Gal- phenotype of snf1 mig1 srb8/10/11 cells."
    Balciunas D., Ronne H.
    Mol. Gen. Genet. 262:589-599(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-622; SER-707 AND SER-720, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-165; SER-304; SER-616 AND SER-622, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGIS4_YEAST
AccessioniPrimary (citable) accession number: Q04233
Secondary accession number(s): D6VZG9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 29, 2015
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 222 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.