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Q04233 (GIS4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein GIS4
Gene names
Name:GIS4
Ordered Locus Names:YML006C
ORF Names:YM9571.13C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length774 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be involved in the Ras/cAMP signaling pathway. Ref.3

Subcellular location

Cytoplasm Ref.4.

Miscellaneous

Present with 222 molecules/cell in log phase SD medium.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 774774Protein GIS4
PRO_0000087498

Regions

Compositional bias147 – 1526Poly-Asn
Compositional bias183 – 20321Thr-rich
Compositional bias329 – 35830Ser-rich
Compositional bias340 – 34910Poly-Ser
Compositional bias353 – 3586Poly-Ser
Compositional bias576 – 58914Asp-rich

Amino acid modifications

Modified residue1651Phosphoserine Ref.8
Modified residue3041Phosphoserine Ref.8
Modified residue6161Phosphoserine Ref.8
Modified residue6221Phosphoserine Ref.6 Ref.8
Modified residue7071Phosphoserine Ref.6
Modified residue7201Phosphoserine Ref.6

Sequences

Sequence LengthMass (Da)Tools
Q04233 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: C7E837AA7001E241

FASTA77487,140
        10         20         30         40         50         60 
MQKSVRVGDY FDNDDNGLWS WYLTNLRLGD FEELIGNQLK YTLLKRFLNS HFYGDNNISA 

        70         80         90        100        110        120 
RPNKKILLVS IPENVHEDIS ILEIFLKDYF HLEKLEHIQI SKLTHSHCYN HENHYLLTDN 

       130        140        150        160        170        180 
LNNFQDPTFL EFASTSWQVQ KNSKALNNNN RNSIPPPTIS SSKASNGKLE SNVSDDQWSN 

       190        200        210        220        230        240 
INTQTSTATR TNTNTRTLTS PDTVDINVTS VNSQSNNNDT PQDNENEVDE EDATSSIVLN 

       250        260        270        280        290        300 
FSHSRTVDSK PNRLPKIFPS YTNEDYTPSH SEIMSIDSFA GEDVSSTYPG QDLSLTTARR 

       310        320        330        340        350        360 
EDESGQDEVE DHYSRVSHDL GDESIDQASY SMESSVSYTS YSSSSNSSSA HYSLSSSSRG 

       370        380        390        400        410        420 
NPKRENIDHT NATYVSELSS ITSSIDNLTT STTPEEEDNL IHHNYDAQGY GSGEDDGEEV 

       430        440        450        460        470        480 
YDDEDLSSSD YSVLSILPSI SICDSLGYFR LVLQSILIQD PDTKEIFTAI RQSNNKPTMA 

       490        500        510        520        530        540 
SVTDDWLLYD SNFSMNNLQI LTLQDLLDIK RSFPKILFYT MVIVTNSGKQ VEEEFKNPNY 

       550        560        570        580        590        600 
DNREGISKEQ PLDSELSLTN DPQQYFPTAY NNGYNDYIDD EDDEDDGDDA SLSEQSGPQM 

       610        620        630        640        650        660 
YIPTRMESNV TTAHRSIRTV NSIGEWAFNR HNSVTKIDKS NSNELDNSKT GESTVLSSEP 

       670        680        690        700        710        720 
HPMTQLSNSN TTSSNFSHSL KTKNSHKPNS KGNNESNSKN ELKKIKSSIN AMSAVERSKS 

       730        740        750        760        770 
LPLPTLLKSL SGIDNPTHAT NKDRKRWKFQ MNRFKNHKNS GSAGTDKSQR CAIM 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Yeast genes GIS1-4: multicopy suppressors of the Gal- phenotype of snf1 mig1 srb8/10/11 cells."
Balciunas D., Ronne H.
Mol. Gen. Genet. 262:589-599(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-622; SER-707 AND SER-720, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-165; SER-304; SER-616 AND SER-622, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49810 Genomic DNA. Translation: CAA89946.1.
BK006946 Genomic DNA. Translation: DAA09893.1.
PIRS55113.
RefSeqNP_013708.1. NM_001182361.1.

3D structure databases

ProteinModelPortalQ04233.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35164. 62 interactions.
DIPDIP-1334N.
IntActQ04233. 12 interactions.
MINTMINT-384235.
STRING4932.YML006C.

Proteomic databases

MaxQBQ04233.
PaxDbQ04233.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYML006C; YML006C; YML006C.
GeneID855006.
KEGGsce:YML006C.

Organism-specific databases

CYGDYML006c.
SGDS000004465. GIS4.

Phylogenomic databases

eggNOGNOG45211.
HOGENOMHOG000000749.
OMAKILFYTM.
OrthoDBEOG72ZCPP.

Enzyme and pathway databases

BioCycYEAST:G3O-32611-MONOMER.

Gene expression databases

GenevestigatorQ04233.

Family and domain databases

ProtoNetSearch...

Other

NextBio978166.

Entry information

Entry nameGIS4_YEAST
AccessionPrimary (citable) accession number: Q04233
Secondary accession number(s): D6VZG9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 14, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD