Reviewed, UniProtKB/Swiss-Prot Q04233 (GIS4_YEAST)

Names and origin

Protein names

Recommended name:
    Protein GIS4

Gene names

Name:	GIS4
Ordered Locus Names:	YML006C
ORF Names:	YM9571.13C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	774 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May be involved in the Ras/cAMP signaling pathway. Ref.2
Subcellular location	Cytoplasm Ref.3.
Miscellaneous	Present with 222 molecules/cell in log phase SD medium. Ref.4

Ontologies

Keywords
Cellular component	Cytoplasm
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular ion homeostasis Inferred from mutant phenotype. Source: SGD intracellular signaling cascade Ref.2 Inferred from genetic interaction. Source: SGD
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PFK2	P16862	1	EBI-27721,EBI-9435

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 774	774	Protein GIS4		PRO_0000087498
Regions
	Compositional bias	147 – 152	6	Poly-Asn
	Compositional bias	183 – 203	21	Thr-rich
	Compositional bias	329 – 358	30	Ser-rich
	Compositional bias	340 – 349	10	Poly-Ser
	Compositional bias	353 – 358	6	Poly-Ser
	Compositional bias	576 – 589	14	Asp-rich
Amino acid modifications
	Modified residue	304	1	Phosphoserine Ref.7
	Modified residue	622	1	Phosphoserine Ref.5
	Modified residue	640	1	Phosphoserine Ref.7
	Modified residue	642	1	Phosphoserine Ref.6
	Modified residue	707	1	Phosphoserine Ref.5
	Modified residue	720	1	Phosphoserine Ref.5
	Modified residue	731	1	Phosphoserine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Q04233-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: C7E837AA7001E241	FASTA	774	87,140
	10 20 30 40 50 60 MQKSVRVGDY FDNDDNGLWS WYLTNLRLGD FEELIGNQLK YTLLKRFLNS HFYGDNNISA 70 80 90 100 110 120 RPNKKILLVS IPENVHEDIS ILEIFLKDYF HLEKLEHIQI SKLTHSHCYN HENHYLLTDN 130 140 150 160 170 180 LNNFQDPTFL EFASTSWQVQ KNSKALNNNN RNSIPPPTIS SSKASNGKLE SNVSDDQWSN 190 200 210 220 230 240 INTQTSTATR TNTNTRTLTS PDTVDINVTS VNSQSNNNDT PQDNENEVDE EDATSSIVLN 250 260 270 280 290 300 FSHSRTVDSK PNRLPKIFPS YTNEDYTPSH SEIMSIDSFA GEDVSSTYPG QDLSLTTARR 310 320 330 340 350 360 EDESGQDEVE DHYSRVSHDL GDESIDQASY SMESSVSYTS YSSSSNSSSA HYSLSSSSRG 370 380 390 400 410 420 NPKRENIDHT NATYVSELSS ITSSIDNLTT STTPEEEDNL IHHNYDAQGY GSGEDDGEEV 430 440 450 460 470 480 YDDEDLSSSD YSVLSILPSI SICDSLGYFR LVLQSILIQD PDTKEIFTAI RQSNNKPTMA 490 500 510 520 530 540 SVTDDWLLYD SNFSMNNLQI LTLQDLLDIK RSFPKILFYT MVIVTNSGKQ VEEEFKNPNY 550 560 570 580 590 600 DNREGISKEQ PLDSELSLTN DPQQYFPTAY NNGYNDYIDD EDDEDDGDDA SLSEQSGPQM 610 620 630 640 650 660 YIPTRMESNV TTAHRSIRTV NSIGEWAFNR HNSVTKIDKS NSNELDNSKT GESTVLSSEP 670 680 690 700 710 720 HPMTQLSNSN TTSSNFSHSL KTKNSHKPNS KGNNESNSKN ELKKIKSSIN AMSAVERSKS 730 740 750 760 770 LPLPTLLKSL SGIDNPTHAT NKDRKRWKFQ MNRFKNHKNS GSAGTDKSQR CAIM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Yeast genes GIS1-4: multicopy suppressors of the Gal- phenotype of snf1 mig1 srb8/10/11 cells." Balciunas D., Ronne H. Mol. Gen. Genet. 262:589-599(1999) [PubMed: 10628841] [Abstract] Cited for: FUNCTION.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-622; SER-707 AND SER-720, MASS SPECTROMETRY.
[6]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-642, MASS SPECTROMETRY.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-304; SER-640 AND SER-731, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z49810 Genomic DNA. Translation: CAA89946.1.
PIR	S55113.
RefSeq	NP_013708.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1334N.
IntAct	Q04233. 6 interactions.
STRING	Q04233.
Proteomic databases
PRIDE	Q04233.
Genome annotation databases
Ensembl	YML006C; YML006C; YML006C; Saccharomyces cerevisiae. [Genome view]
GeneID	855006.
KEGG	sce:YML006C.
NMPDR	fig|4932.3.peg.4751.
Organism-specific databases
CYGD	YML006c.
SGD	S000004465. GIS4.
Phylogenomic databases
OMA	RSIRTVN.
OrthoDB	EOG9HQG2T.
Gene expression databases
ArrayExpress	Q04233.
Genevestigator	Q04233.
GermOnline	YML006C. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	978166.

Entry information

Entry name

GIS4_YEAST

Accession

Primary (citable) accession number: Q04233

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	December 15, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)