Q04217 (DHR1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable ATP-dependent RNA helicase DHR1
EC=3.6.4.13

Alternative name(s):
DEAH box RNA helicase DHR1
Extracellular mutant protein 16

Gene names

Name:	ECM16
Synonyms:	DHR1
Ordered Locus Names:	YMR128W
ORF Names:	YM9553.04

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	1267 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable ATP-binding RNA helicase. Required for 18S rRNA synthesis. May play a role in restructuring of the pre-rRNA. Ref.3
Catalytic activity	ATP + H2O = ADP + phosphate.
Subunit structure	Interacts with snoRNA U3. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3. Ref.3 Ref.4
Subcellular location	Nucleus › nucleolus Ref.3.
Miscellaneous	Present with 2000 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the DEAD box helicase family. DEAH subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Biological process	Ribosome biogenesis rRNA processing
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Helicase Hydrolase Ribonucleoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype PubMed 16449634. Source: SGD
Cellular_component	90S preribosome Inferred from direct assay PubMed 12150911. Source: SGD mitochondrion Inferred from direct assay PubMed 14576278PubMed 16823961. Source: SGD nucleolus Inferred from direct assay Ref.3PubMed 22842922. Source: SGD small-subunit processome Inferred from direct assay Ref.4. Source: SGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro RNA binding Inferred from electronic annotation. Source: UniProtKB-KW RNA helicase activity Inferred from direct assay Ref.3. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1267	1267	Probable ATP-dependent RNA helicase DHR1		PRO_0000055162
Regions
	Domain	401 – 580	180	Helicase ATP-binding
	Domain	675 – 858	184	Helicase C-terminal
	Nucleotide binding	414 – 421	8	ATP By similarity
	Motif	516 – 519	4	DEAH box

Sequences

	Sequence		Length	Mass (Da)
	Q04217 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 576DAAE6D934CC77	FASTA	1,267	144,955
	10 20 30 40 50 60 MGTYRKRFNE KARSGHMAKL KELKRIRNKQ FTRQDENDER VENPDSAPAE SSTTEPNANA 70 80 90 100 110 120 EILEPLTEEE KKMKKRKLQE LFTPKESKVS RLKKKRLDKF IEHQLKREER KTIIGKLQDY 130 140 150 160 170 180 KIDTSLLTSS KRLGEGRQTK KEEFKEALSL ERQGRGNEQT NEILYEEYEP KVWDEYGEGG 190 200 210 220 230 240 SSEDDDGEDD FEASFGSMPK PTDNEEKKSS GFIDHRPAKF GGSGLSFGFS NIKVINKESK 250 260 270 280 290 300 TPKKKYNWRQ RVEMEELKKH GKEDEMDFDT TSEDDDEEED QEEEDKMHPS ENPLEEVESA 310 320 330 340 350 360 DSETGSEKFD QNDVANEFKD WANQEIKKLE GRDQELVTPT LNIDYKPIIR KEDLDDGLQE 370 380 390 400 410 420 AYVPINENST RKAFYVEVSR SDEIQKARIQ LPVFGEEHKI MEAIHHNDVV IICGETGSGK 430 440 450 460 470 480 TTQVPQFLYE AGFGAEDSPD YPGMVGITQP RRVAAVSMAE RVANELGDHG HKVGYQIRFD 490 500 510 520 530 540 STAKEDTKVK FMTDGVLLRE MMHDFKLTKY SSIIIDEAHE RNINTDILIG MLSRCVRLRA 550 560 570 580 590 600 KLHKENPIEH KKLKLIIMSA TLRVSDFSEN KTLFPIAPPV LQVDARQFPV SIHFNRRTAF 610 620 630 640 650 660 NYTDEAFRKT CKIHQKLPPG AILVFLTGQQ EITHMVKRLR KEFPFKKNSK YNKDLETPVS 670 680 690 700 710 720 KMGINSKTTD LEAEDIDFSV QVIDQDKFKS AIRYEEDEGN SGNGEDEEDE EEEGFEEVLT 730 740 750 760 770 780 EGQTANDPLY VLPLYSLLPT KEQMRVFQKP PQGSRLCIVA TNVAETSLTI PGVRYVVDSG 790 800 810 820 830 840 RSKERKYNES NGVQSFEVGW VSKASANQRS GRAGRTGPGH CYRLYSSAVF EHDFEQFSKP 850 860 870 880 890 900 EILRMPVESI VLQMKSMAIH NIINFPFPTP PDRVALSKAI QLLQYLGALD NKEMITEDGK 910 920 930 940 950 960 KMSLFPLSPR FSKMLLVSDE KACLPYIVAI VSALSVGDPF INEFELGINE ISRKPNPDEN 970 980 990 1000 1010 1020 LDDKIREHDE STPGMDPELK KELRSKFYKS RSQFSKLDKF SDVFRLLSVV SAMDYVPKEQ 1030 1040 1050 1060 1070 1080 KEIFMKKNFL RGKLMEEIVK LRKQLMYIIK SNTSKENIAV VIRNEDLKSD IPSVIQIKLL 1090 1100 1110 1120 1130 1140 KQMICAGFVD HVAVRADVLF PDDAKITNRT SIINIPYIPV LATRTPNIED CFVYIHPTSI 1150 1160 1170 1180 1190 1200 LNNLGEMPPK YMLYYSLHLG GNNKTRMNTL CDIASTPLAN IARKGLLLTY SKPLTGQGLK 1210 1220 1230 1240 1250 1260 TVNLSPTERY CYVVPRFGST VDNDLKIGWD LNPIAVHQKK QKGQWTVIKF ITRKGFQTIT GEEKEKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Dhr1p, a putative DEAH-Box RNA helicase, is associated with the box C+D snoRNP U3." Colley A., Beggs J.D., Tollervey D., Lafontaine D.L.J. Mol. Cell. Biol. 20:7238-7246(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH SNORNA U3, SUBCELLULAR LOCATION.
[4]	"A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis." Dragon F., Gallagher J.E.G., Compagnone-Post P.A., Mitchell B.M., Porwancher K.A., Wehner K.A., Wormsley S., Settlage R.E., Shabanowitz J., Osheim Y., Beyer A.L., Hunt D.F., Baserga S.J. Nature 417:967-970(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN SSU PROCESSOME BY MASS SPECTROMETRY.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z48622 Genomic DNA. Translation: CAA88553.1. BK006946 Genomic DNA. Translation: DAA10025.1.
PIR	S53058.
RefSeq	NP_013847.1. NM_001182629.1.
3D structure databases
ProteinModelPortal	Q04217.
SMR	Q04217. Positions 383-642, 708-941.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6722N.
IntAct	Q04217. 28 interactions.
MINT	MINT-614026.
STRING	4932.YMR128W.
Proteomic databases
PaxDb	Q04217.
PeptideAtlas	Q04217.
PRIDE	Q04217.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR128W; YMR128W; YMR128W.
GeneID	855158.
KEGG	sce:YMR128W.
Organism-specific databases
CYGD	YMR128w.
SGD	S000004735. ECM16.
Phylogenomic databases
eggNOG	COG1643.
GeneTree	ENSGT00550000074985.
HOGENOM	HOG000201240.
KO	K14780.
OMA	HQLKREE.
OrthoDB	EOG4KM2B8.
Gene expression databases
Genevestigator	Q04217.
GermOnline	YMR128W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR002464. DNA/RNA_helicase_DEAH_CS. IPR011709. DUF1605. IPR007502. Helicase-assoc_dom. IPR014001. Helicase_ATP-bd. IPR001650. Helicase_C. [Graphical view]
Pfam	PF00270. DEAD. 1 hit. PF04408. HA2. 1 hit. PF00271. Helicase_C. 1 hit. PF07717. OB_NTP_bind. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00847. HA2. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
PROSITE	PS00690. DEAH_ATP_HELICASE. 1 hit. PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	978575.

Entry information

Entry name

DHR1_YEAST

Accession

Primary (citable) accession number: Q04217
Secondary accession number(s): D6VZV1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 122 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program