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Protein

Probable ATP-dependent RNA helicase DHR1

Gene

ECM16

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable ATP-binding RNA helicase. Required for 18S rRNA synthesis. May play a role in restructuring of the pre-rRNA.1 Publication

Miscellaneous

Present with 2000 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi414 – 421ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: GO_Central
  • RNA binding Source: GO_Central
  • RNA helicase activity Source: SGD

GO - Biological processi

  • maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • ribosome biogenesis Source: SGD
  • rRNA methylation Source: Reactome

Keywordsi

Molecular functionHelicase, Hydrolase, Ribonucleoprotein, RNA-binding
Biological processRibosome biogenesis, rRNA processing
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32821-MONOMER
ReactomeiR-SCE-6791226 Major pathway of rRNA processing in the nucleolus and cytosol

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ATP-dependent RNA helicase DHR1 (EC:3.6.4.13)
Alternative name(s):
DEAH box RNA helicase DHR1
Extracellular mutant protein 16
Gene namesi
Name:ECM16
Synonyms:DHR1
Ordered Locus Names:YMR128W
ORF Names:YM9553.04
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR128W
SGDiS000004735 ECM16

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000551621 – 1267Probable ATP-dependent RNA helicase DHR1Add BLAST1267

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei181PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ04217
PaxDbiQ04217
PRIDEiQ04217

PTM databases

iPTMnetiQ04217

Interactioni

Subunit structurei

Interacts with snoRNA U3. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi35305, 69 interactors
ComplexPortaliCPX-1604 Small ribosomal subunit processome, variant 1
CPX-1607 Small ribosomal subunit processome, variant 2
CPX-1608 Small ribosomal subunit processome, variant 3
DIPiDIP-6722N
IntActiQ04217, 34 interactors
MINTiQ04217
STRINGi4932.YMR128W

Structurei

3D structure databases

ProteinModelPortaliQ04217
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini401 – 580Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST180
Domaini675 – 858Helicase C-terminalPROSITE-ProRule annotationAdd BLAST184

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi516 – 519DEAH box4

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000074985
HOGENOMiHOG000201240
InParanoidiQ04217
KOiK14780
OMAiYVFDCGR
OrthoDBiEOG092C2SAZ

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR002464 DNA/RNA_helicase_DEAH_CS
IPR011709 DUF1605
IPR007502 Helicase-assoc_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF04408 HA2, 1 hit
PF00271 Helicase_C, 1 hit
PF07717 OB_NTP_bind, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00847 HA2, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS00690 DEAH_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit

Sequencei

Sequence statusi: Complete.

Q04217-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGTYRKRFNE KARSGHMAKL KELKRIRNKQ FTRQDENDER VENPDSAPAE
60 70 80 90 100
SSTTEPNANA EILEPLTEEE KKMKKRKLQE LFTPKESKVS RLKKKRLDKF
110 120 130 140 150
IEHQLKREER KTIIGKLQDY KIDTSLLTSS KRLGEGRQTK KEEFKEALSL
160 170 180 190 200
ERQGRGNEQT NEILYEEYEP KVWDEYGEGG SSEDDDGEDD FEASFGSMPK
210 220 230 240 250
PTDNEEKKSS GFIDHRPAKF GGSGLSFGFS NIKVINKESK TPKKKYNWRQ
260 270 280 290 300
RVEMEELKKH GKEDEMDFDT TSEDDDEEED QEEEDKMHPS ENPLEEVESA
310 320 330 340 350
DSETGSEKFD QNDVANEFKD WANQEIKKLE GRDQELVTPT LNIDYKPIIR
360 370 380 390 400
KEDLDDGLQE AYVPINENST RKAFYVEVSR SDEIQKARIQ LPVFGEEHKI
410 420 430 440 450
MEAIHHNDVV IICGETGSGK TTQVPQFLYE AGFGAEDSPD YPGMVGITQP
460 470 480 490 500
RRVAAVSMAE RVANELGDHG HKVGYQIRFD STAKEDTKVK FMTDGVLLRE
510 520 530 540 550
MMHDFKLTKY SSIIIDEAHE RNINTDILIG MLSRCVRLRA KLHKENPIEH
560 570 580 590 600
KKLKLIIMSA TLRVSDFSEN KTLFPIAPPV LQVDARQFPV SIHFNRRTAF
610 620 630 640 650
NYTDEAFRKT CKIHQKLPPG AILVFLTGQQ EITHMVKRLR KEFPFKKNSK
660 670 680 690 700
YNKDLETPVS KMGINSKTTD LEAEDIDFSV QVIDQDKFKS AIRYEEDEGN
710 720 730 740 750
SGNGEDEEDE EEEGFEEVLT EGQTANDPLY VLPLYSLLPT KEQMRVFQKP
760 770 780 790 800
PQGSRLCIVA TNVAETSLTI PGVRYVVDSG RSKERKYNES NGVQSFEVGW
810 820 830 840 850
VSKASANQRS GRAGRTGPGH CYRLYSSAVF EHDFEQFSKP EILRMPVESI
860 870 880 890 900
VLQMKSMAIH NIINFPFPTP PDRVALSKAI QLLQYLGALD NKEMITEDGK
910 920 930 940 950
KMSLFPLSPR FSKMLLVSDE KACLPYIVAI VSALSVGDPF INEFELGINE
960 970 980 990 1000
ISRKPNPDEN LDDKIREHDE STPGMDPELK KELRSKFYKS RSQFSKLDKF
1010 1020 1030 1040 1050
SDVFRLLSVV SAMDYVPKEQ KEIFMKKNFL RGKLMEEIVK LRKQLMYIIK
1060 1070 1080 1090 1100
SNTSKENIAV VIRNEDLKSD IPSVIQIKLL KQMICAGFVD HVAVRADVLF
1110 1120 1130 1140 1150
PDDAKITNRT SIINIPYIPV LATRTPNIED CFVYIHPTSI LNNLGEMPPK
1160 1170 1180 1190 1200
YMLYYSLHLG GNNKTRMNTL CDIASTPLAN IARKGLLLTY SKPLTGQGLK
1210 1220 1230 1240 1250
TVNLSPTERY CYVVPRFGST VDNDLKIGWD LNPIAVHQKK QKGQWTVIKF
1260
ITRKGFQTIT GEEKEKK
Length:1,267
Mass (Da):144,955
Last modified:November 1, 1997 - v1
Checksum:i576DAAE6D934CC77
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48622 Genomic DNA Translation: CAA88553.1
BK006946 Genomic DNA Translation: DAA10025.1
PIRiS53058
RefSeqiNP_013847.1, NM_001182629.1

Genome annotation databases

EnsemblFungiiYMR128W; YMR128W; YMR128W
GeneIDi855158
KEGGisce:YMR128W

Similar proteinsi

Entry informationi

Entry nameiDHR1_YEAST
AccessioniPrimary (citable) accession number: Q04217
Secondary accession number(s): D6VZV1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 20, 2018
This is version 166 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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