Q04212 (ARA2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
D-arabinose 1-dehydrogenase
EC=1.1.1.116

Alternative name(s):
NAD(+)-specific D-arabinose dehydrogenase

Gene names

Name:	ARA2
Ordered Locus Names:	YMR041C
ORF Names:	YM9532.06C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	D-arabinose + NAD+ = D-arabinono-1,4-lactone + NADH.
Sequence similarities	Belongs to the aldo/keto reductase 2 family.
Biophysicochemical properties	Kinetic parameters: KM=0.78 mM for D-arabinose Ref.1

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	dehydro-D-arabinono-1,4-lactone biosynthetic process Inferred from mutant phenotype Ref.1. Source: SGD
Molecular function	D-arabinose 1-dehydrogenase (NAD) activity Inferred from electronic annotation. Source: EC D-arabinose 1-dehydrogenase [NAD(P)+] activity Inferred from mutant phenotype Ref.1. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RSP5	P39940	1	EBI-28073,EBI-16219
SSM4	P40318	1	EBI-28073,EBI-18208

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 335	335	D-arabinose 1-dehydrogenase		PRO_0000070372
Regions
	Nucleotide binding	221 – 287	67	NAD Potential
Sites
	Active site	58	1	Proton donor By similarity
	Binding site	124	1	Substrate Potential
	Site	86	1	Lowers pKa of active site Tyr By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q04212 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: CC7FA464FFAE6ED7	FASTA	335	38,220
	10 20 30 40 50 60 MVNEKVNPFD LASVSPLVLG GAILNQQYTD EPESIPLEDI IKYAFSHGIN AIDTSPYYGP 70 80 90 100 110 120 SEVLYGRALS NLRNEFPRDT YFICTKVGRI GAEEFNYSRD FVRFSVHRSC ERLHTTYLDL 130 140 150 160 170 180 VYLHDVEFVK FPDILEALKE LRTLKNKGVI KNFGISGYPI DFITWLAEYC STEESDIGSL 190 200 210 220 230 240 DAVLSYCNLN LQNNKLLNFR ERLLRNAKLK MVCNASILSM SLLRSQETRQ FHPCSHELRE 250 260 270 280 290 300 CASQAAKYCQ EQNVDLADLA TRYAISEWVG KGPVVLGVSS MEELKLALDN YEIVKSNGNR 310 320 330 LSSKDGQLVE YIQKNIFKEH FNEEWSSGIP HPEMI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"NAD+-specific D-arabinose dehydrogenase and its contribution to erythroascorbic acid production in Saccharomyces cerevisiae." Amako K., Fujita K., Shimohata T.A., Hasegawa E., Kishimoto R., Goda K. FEBS Lett. 580:6428-6434(2006) [PubMed: 17097644] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB237161 Genomic DNA. Translation: BAE93049.1. Z48502 Genomic DNA. Translation: CAA88407.1. BK006946 Genomic DNA. Translation: DAA09940.1.
PIR	S52890.
RefSeq	NP_013755.1. NM_001182538.1.
3D structure databases
ProteinModelPortal	Q04212.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4506N.
IntAct	Q04212. 2 interactions.
MINT	MINT-573097.
STRING	Q04212.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR041C; YMR041C; YMR041C.
GeneID	855057.
KEGG	sce:YMR041C.
NMPDR	fig|4932.3.peg.4802.
Organism-specific databases
CYGD	YMR041c.
SGD	S000004644. ARA2.
Phylogenomic databases
eggNOG	fuNOG07888.
GeneTree	EFGT00050000001341.
HOGENOM	HBG397216.
OMA	GPQPWHP.
OrthoDB	EOG4Z39Q4.
Enzyme and pathway databases
BRENDA	1.1.1.116. 984.
Gene expression databases
ArrayExpress	Q04212.
Genevestigator	Q04212.
GermOnline	YMR041C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001395. Aldo/ket_red. IPR023210. NADP_OxRdtase_dom. [Graphical view]
Gene3D	G3DSA:3.20.20.100. Aldo/ket_red. 1 hit.
KO	K00062.
PANTHER	PTHR11732. Aldo/ket_red. 1 hit.
Pfam	PF00248. Aldo_ket_red. 1 hit. [Graphical view]
SUPFAM	SSF51430. Aldo/ket_red. 1 hit.
PROSITE	PS00798. ALDOKETO_REDUCTASE_1. False negative. PS00062. ALDOKETO_REDUCTASE_2. False negative. PS00063. ALDOKETO_REDUCTASE_3. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	978307.

Entry information

Entry name

ARA2_YEAST

Accession

Primary (citable) accession number: Q04212
Secondary accession number(s): D6VZL6, Q1XGQ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	December 14, 2011
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program