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Protein

D-arabinose 1-dehydrogenase

Gene

ARA2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-arabinose + NAD+ = D-arabinono-1,4-lactone + NADH.

Kineticsi

  1. KM=0.78 mM for D-arabinose1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei58 – 581Proton donorBy similarity
    Sitei86 – 861Lowers pKa of active site TyrBy similarity
    Binding sitei124 – 1241SubstrateSequence analysis

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi221 – 28767NADSequence analysisAdd
    BLAST

    GO - Molecular functioni

    GO - Biological processi

    • dehydro-D-arabinono-1,4-lactone biosynthetic process Source: SGD
    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER3O-32.
    YEAST:G3O-32746-MONOMER.
    YEAST:MONOMER3O-32.
    BRENDAi1.1.1.116. 984.
    SABIO-RKQ04212.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    D-arabinose 1-dehydrogenase (EC:1.1.1.116)
    Alternative name(s):
    NAD(+)-specific D-arabinose dehydrogenase
    Gene namesi
    Name:ARA2
    Ordered Locus Names:YMR041C
    ORF Names:YM9532.06C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome XIII

    Organism-specific databases

    EuPathDBiFungiDB:YMR041C.
    SGDiS000004644. ARA2.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 335335D-arabinose 1-dehydrogenasePRO_0000070372Add
    BLAST

    Proteomic databases

    MaxQBiQ04212.

    PTM databases

    iPTMnetiQ04212.

    Interactioni

    Protein-protein interaction databases

    BioGridi35213. 14 interactions.
    DIPiDIP-4506N.
    IntActiQ04212. 2 interactions.
    MINTiMINT-573097.

    Structurei

    3D structure databases

    ProteinModelPortaliQ04212.
    SMRiQ04212. Positions 40-158.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    GeneTreeiENSGT00390000005890.
    HOGENOMiHOG000250267.
    InParanoidiQ04212.
    KOiK00062.
    OMAiTTYCSSK.
    OrthoDBiEOG7HF1VS.

    Family and domain databases

    Gene3Di3.20.20.100. 1 hit.
    InterProiIPR001395. Aldo/ket_red/Kv-b.
    IPR023210. NADP_OxRdtase_dom.
    [Graphical view]
    PANTHERiPTHR11732. PTHR11732. 1 hit.
    PfamiPF00248. Aldo_ket_red. 1 hit.
    [Graphical view]
    SUPFAMiSSF51430. SSF51430. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q04212-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MVNEKVNPFD LASVSPLVLG GAILNQQYTD EPESIPLEDI IKYAFSHGIN
    60 70 80 90 100
    AIDTSPYYGP SEVLYGRALS NLRNEFPRDT YFICTKVGRI GAEEFNYSRD
    110 120 130 140 150
    FVRFSVHRSC ERLHTTYLDL VYLHDVEFVK FPDILEALKE LRTLKNKGVI
    160 170 180 190 200
    KNFGISGYPI DFITWLAEYC STEESDIGSL DAVLSYCNLN LQNNKLLNFR
    210 220 230 240 250
    ERLLRNAKLK MVCNASILSM SLLRSQETRQ FHPCSHELRE CASQAAKYCQ
    260 270 280 290 300
    EQNVDLADLA TRYAISEWVG KGPVVLGVSS MEELKLALDN YEIVKSNGNR
    310 320 330
    LSSKDGQLVE YIQKNIFKEH FNEEWSSGIP HPEMI
    Length:335
    Mass (Da):38,220
    Last modified:November 1, 1997 - v1
    Checksum:iCC7FA464FFAE6ED7
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB237161 Genomic DNA. Translation: BAE93049.1.
    Z48502 Genomic DNA. Translation: CAA88407.1.
    BK006946 Genomic DNA. Translation: DAA09940.1.
    PIRiS52890.
    RefSeqiNP_013755.1. NM_001182538.1.

    Genome annotation databases

    EnsemblFungiiYMR041C; YMR041C; YMR041C.
    GeneIDi855057.
    KEGGisce:YMR041C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB237161 Genomic DNA. Translation: BAE93049.1.
    Z48502 Genomic DNA. Translation: CAA88407.1.
    BK006946 Genomic DNA. Translation: DAA09940.1.
    PIRiS52890.
    RefSeqiNP_013755.1. NM_001182538.1.

    3D structure databases

    ProteinModelPortaliQ04212.
    SMRiQ04212. Positions 40-158.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi35213. 14 interactions.
    DIPiDIP-4506N.
    IntActiQ04212. 2 interactions.
    MINTiMINT-573097.

    PTM databases

    iPTMnetiQ04212.

    Proteomic databases

    MaxQBiQ04212.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiYMR041C; YMR041C; YMR041C.
    GeneIDi855057.
    KEGGisce:YMR041C.

    Organism-specific databases

    EuPathDBiFungiDB:YMR041C.
    SGDiS000004644. ARA2.

    Phylogenomic databases

    GeneTreeiENSGT00390000005890.
    HOGENOMiHOG000250267.
    InParanoidiQ04212.
    KOiK00062.
    OMAiTTYCSSK.
    OrthoDBiEOG7HF1VS.

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER3O-32.
    YEAST:G3O-32746-MONOMER.
    YEAST:MONOMER3O-32.
    BRENDAi1.1.1.116. 984.
    SABIO-RKQ04212.

    Miscellaneous databases

    PROiQ04212.

    Family and domain databases

    Gene3Di3.20.20.100. 1 hit.
    InterProiIPR001395. Aldo/ket_red/Kv-b.
    IPR023210. NADP_OxRdtase_dom.
    [Graphical view]
    PANTHERiPTHR11732. PTHR11732. 1 hit.
    PfamiPF00248. Aldo_ket_red. 1 hit.
    [Graphical view]
    SUPFAMiSSF51430. SSF51430. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "NAD+-specific D-arabinose dehydrogenase and its contribution to erythroascorbic acid production in Saccharomyces cerevisiae."
      Amako K., Fujita K., Shimohata T.A., Hasegawa E., Kishimoto R., Goda K.
      FEBS Lett. 580:6428-6434(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.

    Entry informationi

    Entry nameiARA2_YEAST
    AccessioniPrimary (citable) accession number: Q04212
    Secondary accession number(s): D6VZL6, Q1XGQ6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: June 8, 2016
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.