Reviewed, UniProtKB/Swiss-Prot Q04212 (ARA2_YEAST)

Names and origin

Protein names

Recommended name:
    D-arabinose 1-dehydrogenase
    EC=1.1.1.116
Alternative name(s):
    NAD(+)-specific D-arabinose dehydrogenase

Gene names

Name:	ARA2
Ordered Locus Names:	YMR041C
ORF Names:	YM9532.06C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	D-arabinose + NAD(+) = D-arabinono-1,4-lactone + NADH.
Sequence similarities	Belongs to the aldo/keto reductase 2 family.
Biophysicochemical properties	Kinetic parameters: KM=0.78 mM for D-arabinose

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-ascorbic acid biosynthetic process Ref.1 Inferred from mutant phenotype. Source: SGD oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	D-arabinose 1-dehydrogenase [NAD(P)+] activity Ref.1 Inferred from direct assay. Source: SGD D-arabinose 1-dehydrogenase activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RSP5	P39940	1	EBI-28073,EBI-16219
SSM4	P40318	1	EBI-28073,EBI-18208

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 335	335	D-arabinose 1-dehydrogenase		PRO_0000070372
Regions
	Nucleotide binding	221 – 287	67	NAD Potential
Sites
	Active site	58	1	Proton donor By similarity
	Binding site	124	1	Substrate Potential
	Site	86	1	Lowers pKa of active site Tyr By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q04212-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: CC7FA464FFAE6ED7	FASTA	335	38,220
	10 20 30 40 50 60 MVNEKVNPFD LASVSPLVLG GAILNQQYTD EPESIPLEDI IKYAFSHGIN AIDTSPYYGP 70 80 90 100 110 120 SEVLYGRALS NLRNEFPRDT YFICTKVGRI GAEEFNYSRD FVRFSVHRSC ERLHTTYLDL 130 140 150 160 170 180 VYLHDVEFVK FPDILEALKE LRTLKNKGVI KNFGISGYPI DFITWLAEYC STEESDIGSL 190 200 210 220 230 240 DAVLSYCNLN LQNNKLLNFR ERLLRNAKLK MVCNASILSM SLLRSQETRQ FHPCSHELRE 250 260 270 280 290 300 CASQAAKYCQ EQNVDLADLA TRYAISEWVG KGPVVLGVSS MEELKLALDN YEIVKSNGNR 310 320 330 LSSKDGQLVE YIQKNIFKEH FNEEWSSGIP HPEMI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"NAD+-specific D-arabinose dehydrogenase and its contribution to erythroascorbic acid production in Saccharomyces cerevisiae." Amako K., Fujita K., Shimohata T.A., Hasegawa E., Kishimoto R., Goda K. FEBS Lett. 580:6428-6434(2006) [PubMed: 17097644] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB237161 Genomic DNA. Translation: BAE93049.1. Z48502 Genomic DNA. Translation: CAA88407.1.
PIR	S52890.
RefSeq	NP_013755.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4506N.
IntAct	Q04212.
Genome annotation databases
Ensembl	YMR041C. Saccharomyces cerevisiae. [Contig view]
GeneID	855057.
GenomeReviews	Gene locus YMR041C in contig Z71257_GR.
KEGG	sce:YMR041C.
NMPDR	fig|4932.3.peg.4802.
Organism-specific databases
CYGD	YMR041c.
SGD	S000004644. ARA2.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	Q04212.
Gene expression databases
ArrayExpress	Q04212.
GermOnline	YMR041C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001395. Aldo/ket_red. [Graphical view]
Gene3D	G3DSA:3.20.20.100. Aldo/ket_red. 1 hit.
PANTHER	PTHR11732. Aldo/ket_red. 1 hit.
Pfam	PF00248. Aldo_ket_red. 1 hit. [Graphical view]
PRINTS	PR00069. ALDKETRDTASE.
ProDom	PD000288. Aldo/ket_red. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00798. ALDOKETO_REDUCTASE_1. False negative. PS00062. ALDOKETO_REDUCTASE_2. False negative. PS00063. ALDOKETO_REDUCTASE_3. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
LinkHub	Q04212.
NextBio	978307.

Entry information

Entry name

ARA2_YEAST

Accession

Primary (citable) accession number: Q04212
Secondary accession number(s): Q1XGQ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 25, 2008
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)