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Protein

Chromatin assembly factor 1 subunit p60

Gene

CAC2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a component of chromatin assembly factor 1 (CAF-1), which assembles histone octamers onto replicating DNA in vitro. It performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. p50 and p60 form complexes with newly synthesized histones H3 and acetylated H4 in cell extracts (By similarity).By similarity

GO - Biological processi

  1. DNA replication-dependent nucleosome assembly Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-32686-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Chromatin assembly factor 1 subunit p60
Alternative name(s):
CAF-1 60 kDa subunit
Gene namesi
Name:CAC2
Ordered Locus Names:YML102W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XIII

Organism-specific databases

CYGDiYML102w.
SGDiS000004570. CAC2.

Subcellular locationi

GO - Cellular componenti

  1. CAF-1 complex Source: SGD
  2. chromosome, centromeric region Source: SGD
  3. cytosol Source: SGD
  4. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 468468Chromatin assembly factor 1 subunit p60PRO_0000050894Add
BLAST

Proteomic databases

MaxQBiQ04199.
PaxDbiQ04199.
PeptideAtlasiQ04199.

Expressioni

Gene expression databases

GenevestigatoriQ04199.

Interactioni

Subunit structurei

Component of chromatin assembly factor 1 (CAF-1), which is composed of MSI1/p50, CAC2/p60 and CAC1/p90.

Protein-protein interaction databases

BioGridi35041. 119 interactions.
DIPiDIP-811N.
IntActiQ04199. 6 interactions.
MINTiMINT-552288.
STRINGi4932.YML102W.

Structurei

3D structure databases

ProteinModelPortaliQ04199.
SMRiQ04199. Positions 32-214.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati11 – 5242WD 1Add
BLAST
Repeati69 – 10840WD 2Add
BLAST
Repeati143 – 18240WD 3Add
BLAST
Repeati185 – 22440WD 4Add
BLAST
Repeati371 – 41343WD 5Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat HIR1 family.Curated
Contains 5 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiCOG2319.
GeneTreeiENSGT00550000074968.
HOGENOMiHOG000183312.
InParanoidiQ04199.
KOiK10751.
OMAiQDGYCTL.
OrthoDBiEOG7NCVC9.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 5 hits.
[Graphical view]
SMARTiSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q04199-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEASHLQIYW HDSQPVYSLT FQKNSANDKL FTAGGDNKVR IWKLNRDENG
60 70 80 90 100
QNGGVRKIES LDFLGSLTHH EQAINVIRFN SKGDVLASAG DDGQVLLWKQ
110 120 130 140 150
EDPNTQQESV VRPFGMDAET SEADENKEKW VVWKRLRGGS GATAAAEIYD
160 170 180 190 200
LAWSPDNRNI VVACMDNSIR LFDVGAGMLV CGQSDHGHYV QGVAWDPLNQ
210 220 230 240 250
FILSQSADRS LHVYGVILSS AGVVTGLKLR SKIAKAELPC PGDVLRTNYL
260 270 280 290 300
FHNETLPSFF RRCSISPCGG LVVIPSGVYK VAGDEVANCV YVYTRSGILN
310 320 330 340 350
SAGGVKNRPA IRIPSLKKPA LMAAFSPVFY ETCQKSVLKL PYKLVFAIAT
360 370 380 390 400
TNEVLVYDTD VLEPLCVVGN IHYSPITDLA WSEDGSTLLI SSTDGFCSYV
410 420 430 440 450
SIDTETQFGS RIEPPAMHAE PLDTDESAVA AKNQREAGGI VNMLPVKKIP
460
CNSSDSKKRR IHPTPVDL
Length:468
Mass (Da):51,252
Last modified:November 1, 1997 - v1
Checksum:iF24853C4AA4D97C2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80835 Genomic DNA. Translation: CAA56795.1.
BK006946 Genomic DNA. Translation: DAA09796.1.
PIRiS47447.
RefSeqiNP_013605.1. NM_001182464.1.

Genome annotation databases

EnsemblFungiiYML102W; YML102W; YML102W.
GeneIDi854869.
KEGGisce:YML102W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80835 Genomic DNA. Translation: CAA56795.1.
BK006946 Genomic DNA. Translation: DAA09796.1.
PIRiS47447.
RefSeqiNP_013605.1. NM_001182464.1.

3D structure databases

ProteinModelPortaliQ04199.
SMRiQ04199. Positions 32-214.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35041. 119 interactions.
DIPiDIP-811N.
IntActiQ04199. 6 interactions.
MINTiMINT-552288.
STRINGi4932.YML102W.

Proteomic databases

MaxQBiQ04199.
PaxDbiQ04199.
PeptideAtlasiQ04199.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYML102W; YML102W; YML102W.
GeneIDi854869.
KEGGisce:YML102W.

Organism-specific databases

CYGDiYML102w.
SGDiS000004570. CAC2.

Phylogenomic databases

eggNOGiCOG2319.
GeneTreeiENSGT00550000074968.
HOGENOMiHOG000183312.
InParanoidiQ04199.
KOiK10751.
OMAiQDGYCTL.
OrthoDBiEOG7NCVC9.

Enzyme and pathway databases

BioCyciYEAST:G3O-32686-MONOMER.

Miscellaneous databases

NextBioi977801.
PROiQ04199.

Gene expression databases

GenevestigatoriQ04199.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 5 hits.
[Graphical view]
SMARTiSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Ultraviolet radiation sensitivity and reduction of telomeric silencing in Saccharomyces cerevisiae cells lacking chromatin assembly factor-I."
    Kaufman P.D., Kobayashi R., Stillman B.
    Genes Dev. 11:345-357(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 58-83 AND 459-468, CHARACTERIZATION.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCAC2_YEAST
AccessioniPrimary (citable) accession number: Q04199
Secondary accession number(s): D6W0I2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: January 7, 2015
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2130 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.