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Q04199 (CAC2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chromatin assembly factor 1 subunit p60
Alternative name(s):
CAF-1 60 kDa subunit
Gene names
Name:CAC2
Ordered Locus Names:YML102W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length468 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a component of chromatin assembly factor 1 (CAF-1), which assembles histone octamers onto replicating DNA in vitro. It performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. p50 and p60 form complexes with newly synthesized histones H3 and acetylated H4 in cell extracts By similarity.

Subunit structure

Component of chromatin assembly factor 1 (CAF-1), which is composed of MSI1/p50, CAC2/p60 and CAC1/p90.

Subcellular location

Nucleus.

Miscellaneous

Present with 2130 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the WD repeat HIR1 family.

Contains 5 WD repeats.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

RLF2Q124954EBI-3920,EBI-3913

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 468468Chromatin assembly factor 1 subunit p60
PRO_0000050894

Regions

Repeat11 – 5242WD 1
Repeat69 – 10840WD 2
Repeat143 – 18240WD 3
Repeat185 – 22440WD 4
Repeat371 – 41343WD 5

Sequences

Sequence LengthMass (Da)Tools
Q04199 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: F24853C4AA4D97C2

FASTA46851,252
        10         20         30         40         50         60 
MEASHLQIYW HDSQPVYSLT FQKNSANDKL FTAGGDNKVR IWKLNRDENG QNGGVRKIES 

        70         80         90        100        110        120 
LDFLGSLTHH EQAINVIRFN SKGDVLASAG DDGQVLLWKQ EDPNTQQESV VRPFGMDAET 

       130        140        150        160        170        180 
SEADENKEKW VVWKRLRGGS GATAAAEIYD LAWSPDNRNI VVACMDNSIR LFDVGAGMLV 

       190        200        210        220        230        240 
CGQSDHGHYV QGVAWDPLNQ FILSQSADRS LHVYGVILSS AGVVTGLKLR SKIAKAELPC 

       250        260        270        280        290        300 
PGDVLRTNYL FHNETLPSFF RRCSISPCGG LVVIPSGVYK VAGDEVANCV YVYTRSGILN 

       310        320        330        340        350        360 
SAGGVKNRPA IRIPSLKKPA LMAAFSPVFY ETCQKSVLKL PYKLVFAIAT TNEVLVYDTD 

       370        380        390        400        410        420 
VLEPLCVVGN IHYSPITDLA WSEDGSTLLI SSTDGFCSYV SIDTETQFGS RIEPPAMHAE 

       430        440        450        460 
PLDTDESAVA AKNQREAGGI VNMLPVKKIP CNSSDSKKRR IHPTPVDL

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Ultraviolet radiation sensitivity and reduction of telomeric silencing in Saccharomyces cerevisiae cells lacking chromatin assembly factor-I."
Kaufman P.D., Kobayashi R., Stillman B.
Genes Dev. 11:345-357(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 58-83 AND 459-468, CHARACTERIZATION.
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X80835 Genomic DNA. Translation: CAA56795.1.
BK006946 Genomic DNA. Translation: DAA09796.1.
PIRS47447.
RefSeqNP_013605.1. NM_001182464.1.

3D structure databases

ProteinModelPortalQ04199.
SMRQ04199. Positions 32-214.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-811N.
IntActQ04199. 4 interactions.
MINTMINT-552288.
STRING4932.YML102W.

Proteomic databases

PaxDbQ04199.
PeptideAtlasQ04199.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYML102W; YML102W; YML102W.
GeneID854869.
KEGGsce:YML102W.

Organism-specific databases

CYGDYML102w.
SGDS000004570. CAC2.

Phylogenomic databases

eggNOGCOG2319.
GeneTreeENSGT00550000074968.
HOGENOMHOG000183312.
KOK10751.
OMAVYDICWT.
OrthoDBEOG4643MT.

Gene expression databases

GenevestigatorQ04199.
GermOnlineYML102W. Saccharomyces cerevisiae.

Family and domain databases

Gene3D2.130.10.10. 1 hit.
InterProIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamPF00400. WD40. 5 hits.
[Graphical view]
SMARTSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMSSF50978. WD40_like. 1 hit.
PROSITEPS00678. WD_REPEATS_1. False negative.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio977801.

Entry information

Entry nameCAC2_YEAST
AccessionPrimary (citable) accession number: Q04199
Secondary accession number(s): D6W0I2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 1, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

SIMILARITY comments

Index of protein domains and families

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