Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q04183

- TR120_YEAST

UniProt

Q04183 - TR120_YEAST

Protein

Trafficking protein particle complex II-specific subunit 120

Gene

TRS120

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Specific subunit of the TRAPP II complex, a highly conserved vesicle tethering complex that functions in the late Golgi as a guanine nucleotide exchanger (GEF) for the Golgi YPT1 GTPase. TRS120 plays a role in the YPT GEF activity of TRAPP II in concert with the two other TRAPP II-specific subunits TRS65 and TRS130.3 Publications

    GO - Biological processi

    1. early endosome to Golgi transport Source: SGD
    2. intra-Golgi vesicle-mediated transport Source: SGD
    3. positive regulation of Rab GTPase activity Source: GOC
    4. regulation of Rab GTPase activity Source: SGD

    Keywords - Biological processi

    Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29951-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trafficking protein particle complex II-specific subunit 120
    Short name:
    TRAPP II-specific subunit 120
    Alternative name(s):
    Transport protein particle 120 kDa subunit
    Gene namesi
    Name:TRS120
    Ordered Locus Names:YDR407C
    ORF Names:D9509.25, ESBP10
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDR407c.
    SGDiS000002815. TRS120.

    Subcellular locationi

    Golgi apparatuscis-Golgi network 1 Publication

    GO - Cellular componenti

    1. early endosome Source: SGD
    2. trans-Golgi network Source: SGD
    3. TRAPPII protein complex Source: SGD

    Keywords - Cellular componenti

    Golgi apparatus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12891289Trafficking protein particle complex II-specific subunit 120PRO_0000076355Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei379 – 3791Phosphoserine1 Publication
    Modified residuei387 – 3871Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ04183.
    PaxDbiQ04183.
    PeptideAtlasiQ04183.

    Expressioni

    Gene expression databases

    GenevestigatoriQ04183.

    Interactioni

    Subunit structurei

    Part of the multisubunit TRAPP (transport protein particle) II complex composed of BET3, BET5, TRS20, TRS23, TRS31, TRS33, TRS65, TRS120 and TRS130. Interacts directly with TRS65.4 Publications

    Protein-protein interaction databases

    BioGridi32467. 22 interactions.
    DIPiDIP-6461N.
    IntActiQ04183. 4 interactions.
    MINTiMINT-669053.
    STRINGi4932.YDR407C.

    Structurei

    3D structure databases

    ProteinModelPortaliQ04183.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the TRS120 family.Curated

    Phylogenomic databases

    eggNOGiNOG307918.
    HOGENOMiHOG000142077.
    OMAiETIMCDI.
    OrthoDBiEOG7DRJBS.

    Family and domain databases

    InterProiIPR013935. TRAPP_II_complex_Trs120.
    [Graphical view]
    PfamiPF08626. TRAPPC9-Trs120. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q04183-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNILKHFPSY VGPSKIRTLV IPIGHWTRKE FNNAVQKLSE FNEIHLSDVT     50
    PIDSPIFTPQ GFPHGKLFFD FLTIDHDDAL ELFLYDFEPF RKTFVIIGLV 100
    NDYSDPLTNL NFMKEKYPTL ISPNLVYASS TPTKELEQTI DTMENVFASS 150
    PDMQKNIETI MCDIARNFLT ALNSYYSSYK HVTLRSPGAI GGNAVLKTTL 200
    IRQNSYTSSS SSTPMSAVQS SVSSSSKAGS VTTASKRLSS FEMTTNSLKR 250
    SASLKLATTL STSENRSQQK SLGRQMKILG NFQLLAGRYV DALNSFVDAI 300
    TTLYKVRDYL WLGSALDGIS ICFLLLSYLG LSYQIPQIVS LICPVEKLNF 350
    ESSSTGISPV DSNSKATAST TASSTPRNSI SIAAMQSPRN SIMSLSAPAL 400
    NIDVENINLP LLIKCISDKV LYYYDLSLMH NSEYAPQVVY CEFLLKTLTF 450
    MTSCYKSSEF SKDVLDNIVK NQHRALSDIP NSPMFPRFEV YFYSNKLFEL 500
    QLKEMQVEAQ IKIYSTMAEV YRLLGYKRKQ LFVLRLLMVA LLATPNKIAW 550
    HPDYRTLIDT IIELLNINES EAKINVDDPS QSTWLILQKK ILQLCIKVSR 600
    KINDFEYVAK FSSILITKYT HLLNQSEQDA LFKEYIQPSI TNESITSYWD 650
    PFILREVVIN RILDSDPTSN EIPLESDVSS LESLENRQKT QDINPQEVFN 700
    PFKRVQPTSF VSNNSTKVPI LVFLVGDKAE FTCRVQNPFK FDFTINDIQL 750
    DEEISEFCEI DRKAVSYSGP YNVKAESIRS ITLPLIIKKP TYKKIYEISC 800
    LKISILKLPL QKFDIINDSR RSNPVEEEAE YSKCIYGKLK IKILPEQPQL 850
    ELLSTSKMTR NSWMMLDGTK TDFHITVRNK SLSCAINHIK IIPMNNIEQM 900
    LKPDYWKKMP PDDLYIMEKQ LDWLSKSCVR IIKLPTVIKP NETITFDLEL 950
    DNTAVPFNFT GFDLLIEYGM SATDESCIYL KKLSIPYEVT LRRTIEVPSM 1000
    DIIPLNELFS SQVENVDWIE YVMSKIRAES NLHSRDFILL LLDFRNSWID 1050
    GIKLNVQFED FTSNEYHVEA SHTSRIIVPI KKIDYKKYNF ENTPIPRIYP 1100
    GRQFIQSGLN EEQTIEMRQK FWCREHIISK LKCNWKLTTD QSVTGSVDFN 1150
    KFIEKFDHKM VYTIYPGRLF YGVQLLLDEP KVKVGEIINL KIITEPTSTC 1200
    RRKQNSTVNF LDIVIFDSKT SKILPRSNRR ILYNGSLTKP ISTTKVSEIN 1250
    LEIIPIEKGR YEFSVCISKS NNQDGIIQFD SENVILSVI 1289
    Length:1,289
    Mass (Da):147,662
    Last modified:November 1, 1996 - v1
    Checksum:iA1B01959A5875E29
    GO

    Sequence cautioni

    The sequence CAA59326.1 differs from that shown. Reason: Frameshift at position 61.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti435 – 4351A → C in CAA59326. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U32274 Genomic DNA. Translation: AAB64847.1.
    X84902 Genomic DNA. Translation: CAA59326.1. Sequence problems.
    BK006938 Genomic DNA. Translation: DAA12249.1.
    PIRiS69689.
    RefSeqiNP_010695.1. NM_001180715.1.

    Genome annotation databases

    EnsemblFungiiYDR407C; YDR407C; YDR407C.
    GeneIDi852016.
    KEGGisce:YDR407C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U32274 Genomic DNA. Translation: AAB64847.1 .
    X84902 Genomic DNA. Translation: CAA59326.1 . Sequence problems.
    BK006938 Genomic DNA. Translation: DAA12249.1 .
    PIRi S69689.
    RefSeqi NP_010695.1. NM_001180715.1.

    3D structure databases

    ProteinModelPortali Q04183.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32467. 22 interactions.
    DIPi DIP-6461N.
    IntActi Q04183. 4 interactions.
    MINTi MINT-669053.
    STRINGi 4932.YDR407C.

    Proteomic databases

    MaxQBi Q04183.
    PaxDbi Q04183.
    PeptideAtlasi Q04183.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDR407C ; YDR407C ; YDR407C .
    GeneIDi 852016.
    KEGGi sce:YDR407C.

    Organism-specific databases

    CYGDi YDR407c.
    SGDi S000002815. TRS120.

    Phylogenomic databases

    eggNOGi NOG307918.
    HOGENOMi HOG000142077.
    OMAi ETIMCDI.
    OrthoDBi EOG7DRJBS.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29951-MONOMER.

    Miscellaneous databases

    NextBioi 970225.

    Gene expression databases

    Genevestigatori Q04183.

    Family and domain databases

    InterProi IPR013935. TRAPP_II_complex_Trs120.
    [Graphical view ]
    Pfami PF08626. TRAPPC9-Trs120. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Mai B., Lipp M.
      Submitted (FEB-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 40-731.
    4. "TRAPP, a highly conserved novel complex on the cis-Golgi that mediates vesicle docking and fusion."
      Sacher M., Jiang Y., Barrowman J., Scarpa A., Burston J., Zhang L., Schieltz D., Yates J.R. III, Abeliovich H., Ferro-Novick S.
      EMBO J. 17:2494-2503(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE TRAPP II COMPLEX.
    5. "TRAPP I implicated in the specificity of tethering in ER-to-Golgi transport."
      Sacher M., Barrowman J., Wang W., Horecka J., Zhang Y., Pypaert M., Ferro-Novick S.
      Mol. Cell 7:433-442(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE TRAPP II COMPLEX, IDENTIFICATION IN THE TRAPP II COMPLEX, SUBCELLULAR LOCATION.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    8. "The role of Trs65 in the Ypt/Rab guanine nucleotide exchange factor function of the TRAPP II complex."
      Liang Y., Morozova N., Tokarev A.A., Mulholland J.W., Segev N.
      Mol. Biol. Cell 18:2533-2541(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH TRS65.
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-379 AND SER-387, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Molecular architecture of the TRAPPII complex and implications for vesicle tethering."
      Yip C.K., Berscheminski J., Walz T.
      Nat. Struct. Mol. Biol. 17:1298-1304(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE TRAP II COMPLEX, FUNCTION OF THE TRAP II COMPLEX.

    Entry informationi

    Entry nameiTR120_YEAST
    AccessioniPrimary (citable) accession number: Q04183
    Secondary accession number(s): D6VT39, Q05731
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 7, 2006
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 259 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3