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Protein

Importin beta SMX1

Gene

SXM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Nuclear transport factor (karyopherin) involved in protein transport between the cytoplasm and nucleoplasm. Required for the nuclear import of ribosomal proteins (RPL11, RPL16, RPL25, RPL31A), the poly(A)-binding protein PAB1, the HO endonuclease or the tRNA and snRNA chaperone LHP1. Indirectly involved in nuclear mRNA export through its PAB1 nuclear import activity.7 Publications

GO - Molecular functioni

  • protein transporter activity Source: SGD

GO - Biological processi

  • mRNA export from nucleus Source: SGD
  • nucleocytoplasmic transport Source: SGD
  • protein import into nucleus Source: GO_Central
  • signal transduction Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

mRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-29943-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Importin beta SMX1
Alternative name(s):
Suppressor of mRNA export mutant protein 1
karyopherin-108
Gene namesi
Name:SXM1
Synonyms:KAP108
Ordered Locus Names:YDR395W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR395W.
SGDiS000002803. SXM1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • cytosol Source: GO_Central
  • nuclear envelope Source: GO_Central
  • nuclear pore Source: UniProtKB-SubCell
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nuclear pore complex, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 944944Importin beta SMX1PRO_0000269756Add
BLAST

Proteomic databases

MaxQBiQ04175.

PTM databases

iPTMnetiQ04175.

Interactioni

Subunit structurei

Associates with the nucleopore complex. Interacts with LHP1, PAB1, RPL11, RPL16, RPL25, RPL31A in order to import them into the nucleus. Interacts also with the nucleopore complex proteins (nucleoporins) NSP1, NUP1, NUP116 and NUP159.4 Publications

Protein-protein interaction databases

BioGridi32457. 75 interactions.
DIPiDIP-1678N.
IntActiQ04175. 30 interactions.
MINTiMINT-403908.

Structurei

3D structure databases

ProteinModelPortaliQ04175.
SMRiQ04175. Positions 19-596.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 9773Importin N-terminalPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the importin beta family.Curated
Contains 1 importin N-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00550000074736.
HOGENOMiHOG000093618.
InParanoidiQ04175.
OMAiHMISFLE.
OrthoDBiEOG7QK0MD.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR013713. Cse1.
IPR001494. Importin-beta_N.
[Graphical view]
PfamiPF08506. Cse1. 1 hit.
PF03810. IBN_N. 1 hit.
[Graphical view]
SMARTiSM00913. IBN_N. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50166. IMPORTIN_B_NT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q04175-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVQEQAILSC IEQTMVADAK IIKEAEQQLF EFQKQPGFTS FLLNIVSDDN
60 70 80 90 100
FALNVRLSSA IYLKNKIHRS WDTKREDGIK ADEKLSIKER LIETLVKNCE
110 120 130 140 150
NNHIRPILTE TINGILVGQE DWDLAPIIKN LLSSGDASYI YPGLLLLFQL
160 170 180 190 200
CKAHRWDMVG SRDYIDSVIE ELFPIVEGIA SNIGSQTDYR SNEILYLILK
210 220 230 240 250
SFKYACLNNL PQYFSQPERI MSWVQLHLYL CSKPLPVEVM ELDPADRSLD
260 270 280 290 300
KRVKVNKWGF GNLNRFLQRY NKITKAITKE FIDYIFNTIV PIILREFFKD
310 320 330 340 350
IEAWGNNSLW LSDSSLYFLI SFLEKCVTID QLYPLIEPHL QIIFENVIFP
360 370 380 390 400
CLCANEQSIE LLEDDQEEYT RRYFDINREG STPDAASADF IFLIGSKRPE
410 420 430 440 450
KLNNILPFIN DIFTRFDANS SDINMAFKEE GALRTLSNLF SFIDEPSVLE
460 470 480 490 500
NIFGHFIVPL LSQDKYMFLV ARSLETIALY SEEFKDMNIL SQLFELTYTN
510 520 530 540 550
FLNSNVLPVQ IEAADAIKCL IVSNPQIHPA VSAHVPGMME KLLKLSKIFE
560 570 580 590 600
IDILSEVMEA LVERFSDELS PFAKDLASNL VEQFLRIAQA LVENPSETYS
610 620 630 640 650
ASDQEQEIQA SGLLQTMTTM VMSMNKVPLI ESLAPVVKFV VLHAQISFIT
660 670 680 690 700
EAVDLLDALT ISSHLLYNQI APPIWELLHD ILDSFQTYAM DYFEAYSIFF
710 720 730 740 750
ETIVMTGFPQ DQTYVQPLLE ILSAKLESEV DYDIEHVMQI LMYFALSMRD
760 770 780 790 800
IPLFSKAIKV STNDELGLDS KCIVKLGLAN LFAKPIETLQ IMENEGFTIN
810 820 830 840 850
FFTNWFNEKF YSVFAIKLQV LVILTLLKMP EVPNSVSPLL NNLTNKLVEL
860 870 880 890 900
TLSLPKAIRN RDAVTEGKSL EGDLTPEEEE EYFIECDDDM KETVLDQINV
910 920 930 940
FQEVHTFFKN LQNEDAGKYE KIINYLDESK RDSLQVILEF VSQH
Length:944
Mass (Da):108,405
Last modified:November 1, 1996 - v1
Checksum:i33D6E0524AF0568A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U32274 Genomic DNA. Translation: AAB64837.1.
BK006938 Genomic DNA. Translation: DAA12239.1.
PIRiS69679.
RefSeqiNP_010683.3. NM_001180703.3.

Genome annotation databases

EnsemblFungiiYDR395W; YDR395W; YDR395W.
GeneIDi852004.
KEGGisce:YDR395W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U32274 Genomic DNA. Translation: AAB64837.1.
BK006938 Genomic DNA. Translation: DAA12239.1.
PIRiS69679.
RefSeqiNP_010683.3. NM_001180703.3.

3D structure databases

ProteinModelPortaliQ04175.
SMRiQ04175. Positions 19-596.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32457. 75 interactions.
DIPiDIP-1678N.
IntActiQ04175. 30 interactions.
MINTiMINT-403908.

PTM databases

iPTMnetiQ04175.

Proteomic databases

MaxQBiQ04175.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR395W; YDR395W; YDR395W.
GeneIDi852004.
KEGGisce:YDR395W.

Organism-specific databases

EuPathDBiFungiDB:YDR395W.
SGDiS000002803. SXM1.

Phylogenomic databases

GeneTreeiENSGT00550000074736.
HOGENOMiHOG000093618.
InParanoidiQ04175.
OMAiHMISFLE.
OrthoDBiEOG7QK0MD.

Enzyme and pathway databases

BioCyciYEAST:G3O-29943-MONOMER.

Miscellaneous databases

PROiQ04175.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR013713. Cse1.
IPR001494. Importin-beta_N.
[Graphical view]
PfamiPF08506. Cse1. 1 hit.
PF03810. IBN_N. 1 hit.
[Graphical view]
SMARTiSM00913. IBN_N. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50166. IMPORTIN_B_NT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Importin/karyopherin protein family members required for mRNA export from the nucleus."
    Seedorf M., Silver P.A.
    Proc. Natl. Acad. Sci. U.S.A. 94:8590-8595(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "A nuclear import pathway for a protein involved in tRNA maturation."
    Rosenblum J.S., Pemberton L.F., Blobel G.
    J. Cell Biol. 139:1655-1661(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH LHP1; RPL16; ROL25; RPL31A; NSP1; NUP1 AND NUP159.
  5. "Nuclear import and the evolution of a multifunctional RNA-binding protein."
    Rosenblum J.S., Pemberton L.F., Bonifaci N., Blobel G.
    J. Cell Biol. 143:887-899(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH LHP1 AND RPL11.
  6. "Interactions between a nuclear transporter and a subset of nuclear pore complex proteins depend on Ran GTPase."
    Seedorf M., Damelin M., Kahana J., Taura T., Silver P.A.
    Mol. Cell. Biol. 19:1547-1557(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH NSP1; NUP116 AND NUP159.
  7. "Nuclear import of zinc binuclear cluster proteins proceeds through multiple, overlapping transport pathways."
    Nikolaev I., Cochet M.-F., Felenbok B.
    Eukaryot. Cell 2:209-221(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. "Importin 7 and importin alpha/importin beta are nuclear import receptors for the glucocorticoid receptor."
    Freedman N.D., Yamamoto K.R.
    Mol. Biol. Cell 15:2276-2286(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Yeast poly(A)-binding protein Pab1 shuttles between the nucleus and the cytoplasm and functions in mRNA export."
    Brune C., Munchel S.E., Fischer N., Podtelejnikov A.V., Weis K.
    RNA 11:517-531(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PAB1.
  12. "Nuclear import of Ho endonuclease utilizes two nuclear localization signals and four importins of the ribosomal import system."
    Bakhrat A., Baranes K., Krichevsky O., Rom I., Schlenstedt G., Pietrokovski S., Raveh D.
    J. Biol. Chem. 281:12218-12226(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  13. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSXM1_YEAST
AccessioniPrimary (citable) accession number: Q04175
Secondary accession number(s): D6VT29
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: November 1, 1996
Last modified: July 6, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 16300 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.