Reviewed, UniProtKB/Swiss-Prot Q04164 (SAS_DROME)

Names and origin

Protein names

Recommended name:
    Putative epidermal cell surface receptor
Alternative name(s):
    Stranded at second protein

Gene names

Name:	sas
ORF Names:	CG2507

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1693 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Vital for larval development.
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Expressed in most, if not all, ectodermal tissues which produce a cuticle.
Developmental stage	Throughout development.
Sequence similarities	Contains 1 fibronectin type-III domain. Contains 2 VWFC domains.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Repeat Signal Transmembrane
Molecular function	Developmental protein Receptor
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	instar larval development Ref.1 Inferred from mutant phenotype. Source: UniProtKB
Cellular component	apical plasma membrane Non-traceable author statement. Source: FlyBase integral to plasma membrane Ref.1 Non-traceable author statement. Source: UniProtKB
Molecular function	protein binding Inferred from physical interaction. Source: IntAct receptor activity Ref.1 Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q9W1Q1	1	EBI-258615,EBI-101642

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Long (identifier: Q04164-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: Q04164-2)

The sequence of this isoform differs from the canonical sequence as follows:
     930-1274: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 41	41	Potential
	Chain	42 – 1693	1652	Putative epidermal cell surface receptor		PRO_0000022281
Regions
	Topological domain	42 – 1635	1594	Extracellular Potential
	Transmembrane	1636 – 1656	21	Potential
	Topological domain	1657 – 1693	37	Cytoplasmic Potential
	Domain	663 – 708	46	VWFC 1
	Domain	828 – 902	75	VWFC 2
	Domain	1411 – 1501	91	Fibronectin type-III
	Compositional bias	51 – 377	327	Thr-rich
	Compositional bias	534 – 901	368	Cys-rich
	Compositional bias	997 – 1273	277	Glu/Pro-rich
Amino acid modifications
	Glycosylation	106	1	N-linked (GlcNAc...) Potential
	Glycosylation	109	1	N-linked (GlcNAc...) Potential
	Glycosylation	330	1	N-linked (GlcNAc...) Potential
	Glycosylation	473	1	N-linked (GlcNAc...) Potential
	Glycosylation	538	1	N-linked (GlcNAc...) Potential
	Glycosylation	622	1	N-linked (GlcNAc...) Potential
	Glycosylation	685	1	N-linked (GlcNAc...) Potential
	Glycosylation	827	1	N-linked (GlcNAc...) Potential
	Glycosylation	846	1	N-linked (GlcNAc...) Potential
	Glycosylation	929	1	N-linked (GlcNAc...) Potential
	Glycosylation	939	1	N-linked (GlcNAc...) Potential
	Glycosylation	1323	1	N-linked (GlcNAc...) Potential
	Glycosylation	1419	1	N-linked (GlcNAc...) Potential
	Glycosylation	1517	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	930 – 1274	345	Missing in isoform Short.		VSP_004071
Experimental info
	Sequence conflict	591	1	V → L in AAA28879. Ref.1
	Sequence conflict	1509	1	V → G in AAA28879. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified November 15, 2002. Version 2. Checksum: DA50F96677F41DC4	FASTA	1,693	185,254
	10 20 30 40 50 60 MQTCRRRKAS GGQSTIKWSR MCLATLCGLL LLGIQIERAA SAPAGEDAAA TTMPPLDTTT 70 80 90 100 110 120 DAPDAVAATT TPATTAAEQS SSISSITTEA ADGSTTSTTT TTEAANKSNA TETDFTTNVP 130 140 150 160 170 180 VASSLPEETS VRSTSIEPIT STEPTTTPRQ ETEGPDQHMV FSNTEPDQSH IQHIPLRDEH 190 200 210 220 230 240 AESSGADDAT TEMQRQREQD QQQNELNQIS NEQDDVVKDL NNFRHPATLI TASNSNSEEN 250 260 270 280 290 300 VEIESDKQVE TTTTAVPAAA TSTSTEATGT PPTGTPATST STVPNEREED PYHVHILSEN 310 320 330 340 350 360 HDRLAEHEDY QMLSTSTEES STTSTTSTTN STTESGIVAG IVVSQENKAT AEPSTATEST 370 380 390 400 410 420 SISTSTTTAA TAATSTTSRA RAMHMNDPED EAATTIMPDS ESVPVINIVE GQHMLQQEDQ 430 440 450 460 470 480 KDEEEEGVVK ESESSSTTEA STTTTEPSPF VAFAGEGRSA GGGNDIELFL HHNGSTHEQL 490 500 510 520 530 540 MDLSDVSMDG DQNEGSSKTE SSTTSTTTTT AQPETEMPKI VEITASGDTM QRECLANNKS 550 560 570 580 590 600 YKHGELMERD CDERCTCNRG DWMCEPRCRG LSYPRGSQRS MANPNCLEKM VEEDECCRVM 610 620 630 640 650 660 ECSEPQLEPT VVATEGAAPS TNGTGESAVT LPTTDDEATP KPRTDCHYNS GVYKFRERLE 670 680 690 700 710 720 IGCEQICHCA EGGVMDCRPR CPERNHTRLD KCVYVKDPKD VCCQLELCDV TLDDHEQQPT 730 740 750 760 770 780 PLQSNNNEDP EEIDPFRFQE QARDAGGAKP TCTFKGAEYD VGQQFRDGCD QLCICNEQGI 790 800 810 820 830 840 HCAKLECPSN FGLDVQDPHC IRWEPVPADF KPSPPNCCPE SMRCVDNGTC SYQGVQIENW 850 860 870 880 890 900 SPVPANLTGC DQHCYCENGR VECRAACPPV PALPPADLPC HPALARLLPI PDDECCKHWM 910 920 930 940 950 960 CAPQIPKIGG AGQDEETEAT STHSSIPANE TTTTTATANK STSIPSKVPQ IKKDEEKRPP 970 980 990 1000 1010 1020 ASGAFYPTLD GKPPKSIGGL GIFEKPEKPE KAHKKVQHQQ QQHQQQEQQE QQQHQNDVIF 1030 1040 1050 1060 1070 1080 DGDRTEEQEE PLPPNGGFVP FQFGQQHPHQ PHLGPYGFYN PVKPVYEDYN PYEPYDINPN 1090 1100 1110 1120 1130 1140 GTPQGKPPPV PTSQSDLFNI LGAEQPGHPV HPGHGGPPRI HPGQTQKDNH NLGPQVRIEQ 1150 1160 1170 1180 1190 1200 ILQHLQQTVP GGPPPPPPHQ QHQSLTPQLH PQQQQISQQH PGHYVPIVHS GVPPPPPGHG 1210 1220 1230 1240 1250 1260 IAIVDGQTVA YESYPVIPGL GVPQHHPQQH QTTPQQHLQQ TILPSSSTTS GLSTQASEHS 1270 1280 1290 1300 1310 1320 LHQNQGKLAK QQQSGANNLQ PDIEVHTLEA IDPRSIRIVF TVPQVYVNLH GRVELRYSNG 1330 1340 1350 1360 1370 1380 PSNDTSTWEQ QIFAPPEDLI ATSQMEFDLP SLEPNSLYKV KITLILRDLN SQPTSSIYTV 1390 1400 1410 1420 1430 1440 KTPPERTITP PPPFPDYRPD FQDIFKNVED PELTVSETNA SWLQLTWKKL GDDQMEYVDG 1450 1460 1470 1480 1490 1500 VQLRYKELTG MIYSSTPLIH RTLTSYTIQN LQPDTGYEIG LYYIPLAGHG AELRAGHMIK 1510 1520 1530 1540 1550 1560 VRTAQKVDVY GFDVTVNVTK VKTQSVEISW NGVPYPEDKF VHIYRAIYQS DAGKEDSSVF 1570 1580 1590 1600 1610 1620 KVAKRDSTTG TLIMDLKPGT KYRLWLEMYL TNGNTKKSNV VNFITKPGGP ATPGKTGKLL 1630 1640 1650 1660 1670 1680 TAGTDQPVGD YYGPLVVVSV IAALAIMSTL ALLLIITRRR VHQTASITPP RKSDAAYDNP 1690 SYKVEIQQET MNL
	Isoform Short. Checksum: 66416CA86B7FEC97	FASTA	1,348	147,280
	10 20 30 40 50 60 MQTCRRRKAS GGQSTIKWSR MCLATLCGLL LLGIQIERAA SAPAGEDAAA TTMPPLDTTT 70 80 90 100 110 120 DAPDAVAATT TPATTAAEQS SSISSITTEA ADGSTTSTTT TTEAANKSNA TETDFTTNVP 130 140 150 160 170 180 VASSLPEETS VRSTSIEPIT STEPTTTPRQ ETEGPDQHMV FSNTEPDQSH IQHIPLRDEH 190 200 210 220 230 240 AESSGADDAT TEMQRQREQD QQQNELNQIS NEQDDVVKDL NNFRHPATLI TASNSNSEEN 250 260 270 280 290 300 VEIESDKQVE TTTTAVPAAA TSTSTEATGT PPTGTPATST STVPNEREED PYHVHILSEN 310 320 330 340 350 360 HDRLAEHEDY QMLSTSTEES STTSTTSTTN STTESGIVAG IVVSQENKAT AEPSTATEST 370 380 390 400 410 420 SISTSTTTAA TAATSTTSRA RAMHMNDPED EAATTIMPDS ESVPVINIVE GQHMLQQEDQ 430 440 450 460 470 480 KDEEEEGVVK ESESSSTTEA STTTTEPSPF VAFAGEGRSA GGGNDIELFL HHNGSTHEQL 490 500 510 520 530 540 MDLSDVSMDG DQNEGSSKTE SSTTSTTTTT AQPETEMPKI VEITASGDTM QRECLANNKS 550 560 570 580 590 600 YKHGELMERD CDERCTCNRG DWMCEPRCRG LSYPRGSQRS MANPNCLEKM VEEDECCRVM 610 620 630 640 650 660 ECSEPQLEPT VVATEGAAPS TNGTGESAVT LPTTDDEATP KPRTDCHYNS GVYKFRERLE 670 680 690 700 710 720 IGCEQICHCA EGGVMDCRPR CPERNHTRLD KCVYVKDPKD VCCQLELCDV TLDDHEQQPT 730 740 750 760 770 780 PLQSNNNEDP EEIDPFRFQE QARDAGGAKP TCTFKGAEYD VGQQFRDGCD QLCICNEQGI 790 800 810 820 830 840 HCAKLECPSN FGLDVQDPHC IRWEPVPADF KPSPPNCCPE SMRCVDNGTC SYQGVQIENW 850 860 870 880 890 900 SPVPANLTGC DQHCYCENGR VECRAACPPV PALPPADLPC HPALARLLPI PDDECCKHWM 910 920 930 940 950 960 CAPQIPKIGG AGQDEETEAT STHSSIPANG ANNLQPDIEV HTLEAIDPRS IRIVFTVPQV 970 980 990 1000 1010 1020 YVNLHGRVEL RYSNGPSNDT STWEQQIFAP PEDLIATSQM EFDLPSLEPN SLYKVKITLI 1030 1040 1050 1060 1070 1080 LRDLNSQPTS SIYTVKTPPE RTITPPPPFP DYRPDFQDIF KNVEDPELTV SETNASWLQL 1090 1100 1110 1120 1130 1140 TWKKLGDDQM EYVDGVQLRY KELTGMIYSS TPLIHRTLTS YTIQNLQPDT GYEIGLYYIP 1150 1160 1170 1180 1190 1200 LAGHGAELRA GHMIKVRTAQ KVDVYGFDVT VNVTKVKTQS VEISWNGVPY PEDKFVHIYR 1210 1220 1230 1240 1250 1260 AIYQSDAGKE DSSVFKVAKR DSTTGTLIMD LKPGTKYRLW LEMYLTNGNT KKSNVVNFIT 1270 1280 1290 1300 1310 1320 KPGGPATPGK TGKLLTAGTD QPVGDYYGPL VVVSVIAALA IMSTLALLLI ITRRRVHQTA 1330 1340 SITPPRKSDA AYDNPSYKVE IQQETMNL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Drosophila melanogaster stranded at second (sas) gene encodes a putative epidermal cell surface receptor required for larval development." Schonbaum C.P., Organ E.L., Qu S., Cavener D.R. Dev. Biol. 151:431-445(1992) [PubMed: 1339334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SHORT). Strain: Berkeley. Tissue: Embryo.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M68866 mRNA. Translation: AAA28879.1. AE014297 Genomic DNA. Translation: AAN13346.1. AE014297 Genomic DNA. Translation: AAF54052.2. AY051979 mRNA. Translation: AAK93403.1.
RefSeq	NP_476611.1. NP_731141.1.
UniGene	Dm.1085
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:23080N.
IntAct	Q04164. 2 interactions.
Genome annotation databases
Ensembl	FBgn0002306. Drosophila melanogaster. [Contig view]
GeneID	40861.
KEGG	dme:Dmel_CG2507.
NMPDR	fig|7227.3.peg.11515.
Organism-specific databases
FlyBase	FBgn0002306. sas.
Phylogenomic databases
HOGENOM	Q04164.
OMA	Q04164. NPSYKVE.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-010368-MON.
Gene expression databases
ArrayExpress	Q04164.
GermOnline	CG2507. Drosophila melanogaster.
Family and domain databases
InterPro	IPR008957. Fibronectin_typ-III-like_fold. IPR003961. FN_III. IPR001007. VWF_C. [Graphical view]
Gene3D	G3DSA:2.60.40.30. FN_III-like. 1 hit.
Pfam	PF00041. fn3. 1 hit. [Graphical view]
SMART	SM00060. FN3. 3 hits. SM00214. VWC. 3 hits. [Graphical view]
PROSITE	PS50853. FN3. 3 hits. PS01208. VWFC_1. 1 hit. PS50184. VWFC_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	821008.

Entry information

Entry name

SAS_DROME

Accession

Primary (citable) accession number: Q04164
Secondary accession number(s): Q960M6, Q9VI73

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	November 15, 2002
Last modified:	June 16, 2009
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project