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Protein

Endosomal/prevacuolar sodium/hydrogen exchanger

Gene

NHX1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Endosomal/prevacuolar electroneutral Na+/H+ exchanger which mediates intracellular sequestration of Na+ cations, regulates vacuolar pH and contributes to osmotolerance following sudden exposure to hyperosmotic media. Contributes also to the postdiauxic/stationary phase resistance to osmotic stress and allows for the continued growth of cells until the acquired osmotolerance response can occur. Involved in hygromycin resistance probably through its influence on the electrochemical proton gradient affecting secondarily the entrance of hygromycin. Mediates pH-dependent vesicle trafficking out of the endosome. Contributes to K+ sequestration and homeostasis.13 Publications

GO - Molecular functioni

  • potassium:proton antiporter activity Source: SGD
  • sodium:proton antiporter activity Source: SGD

GO - Biological processi

  • cellular monovalent inorganic cation homeostasis Source: SGD
  • potassium ion transmembrane transport Source: SGD
  • sodium ion transmembrane transport Source: SGD
  • vacuolar acidification Source: SGD
Complete GO annotation...

Keywords - Biological processi

Antiport, Ion transport, Potassium transport, Sodium transport, Transport

Keywords - Ligandi

Potassium, Sodium

Enzyme and pathway databases

BioCyciYEAST:G3O-29984-MONOMER.
ReactomeiR-SCE-425986. Sodium/Proton exchangers.

Protein family/group databases

TCDBi2.A.36.1.12. the monovalent cation:proton antiporter-1 (cpa1) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Endosomal/prevacuolar sodium/hydrogen exchanger
Alternative name(s):
Endosomal/prevacuolar Na(+)/H(+) exchanger
Vacuolar protein sorting-associated protein 44
Gene namesi
Name:NHX1
Synonyms:NHA2, VPL27, VPS44
Ordered Locus Names:YDR456W
ORF Names:D9461.40
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR456W.
SGDiS000002864. NHX1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini22 – 6140LumenalSequence analysisAdd
BLAST
Transmembranei62 – 8221HelicalSequence analysisAdd
BLAST
Topological domaini83 – 853CytoplasmicSequence analysis
Transmembranei86 – 10621HelicalSequence analysisAdd
BLAST
Topological domaini107 – 11711LumenalSequence analysisAdd
BLAST
Transmembranei118 – 13821HelicalSequence analysisAdd
BLAST
Topological domaini139 – 15214CytoplasmicSequence analysisAdd
BLAST
Transmembranei153 – 17321HelicalSequence analysisAdd
BLAST
Topological domaini174 – 18916LumenalSequence analysisAdd
BLAST
Transmembranei190 – 21122HelicalSequence analysisAdd
BLAST
Topological domaini212 – 2176CytoplasmicSequence analysis
Transmembranei218 – 23821HelicalSequence analysisAdd
BLAST
Topological domaini239 – 25820LumenalSequence analysisAdd
BLAST
Transmembranei259 – 27921HelicalSequence analysisAdd
BLAST
Topological domaini280 – 2889CytoplasmicSequence analysis
Transmembranei289 – 30820HelicalSequence analysisAdd
BLAST
Topological domaini309 – 3135LumenalSequence analysis
Transmembranei314 – 33320HelicalSequence analysisAdd
BLAST
Topological domaini334 – 34411CytoplasmicSequence analysisAdd
BLAST
Transmembranei345 – 36420HelicalSequence analysisAdd
BLAST
Topological domaini365 – 37612CytoplasmicSequence analysisAdd
BLAST
Transmembranei377 – 39721HelicalSequence analysisAdd
BLAST
Topological domaini398 – 43134LumenalSequence analysisAdd
BLAST
Transmembranei432 – 45221HelicalSequence analysisAdd
BLAST
Topological domaini453 – 4575CytoplasmicSequence analysis
Transmembranei458 – 47821HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • early endosome Source: SGD
  • fungal-type vacuole membrane Source: SGD
  • integral component of membrane Source: UniProtKB-KW
  • late endosome Source: SGD
  • late endosome membrane Source: UniProtKB-SubCell
  • trans-Golgi network Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi201 – 2011D → N: Impairs protein-trafficking to the vacuole. 1 Publication
Mutagenesisi225 – 2251E → Q: Impairs protein-trafficking to the vacuole. 1 Publication
Mutagenesisi230 – 2301D → N: Impairs protein-trafficking to the vacuole. 1 Publication
Mutagenesisi355 – 3551E → Q: Impairs partially resistance to osmotic stress and hygromycin. 1 Publication
Mutagenesisi357 – 3571F → A, L or I: Impairs resistance to osmotic stress and hygromycin, and blocks protein-trafficking to the vacuole. 1 Publication
Mutagenesisi357 – 3571F → V: Impairs partially resistance to osmotic stress and hygromycin. 1 Publication
Mutagenesisi361 – 3611Y → A: Impairs resistance to osmotic stress and hygromycin, and blocks protein-trafficking to the vacuole. 1 Publication
Mutagenesisi365 – 3651E → A: Impairs resistance to osmotic stress and hygromycin, and blocks protein-trafficking to the vacuole. 1 Publication
Mutagenesisi369 – 3691E → A: Impairs partially resistance to osmotic stress and hygromycin. 1 Publication
Mutagenesisi376 – 3761P → N: Impairs resistance to osmotic stress and hygromycin. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence analysisAdd
BLAST
Chaini22 – 633612Endosomal/prevacuolar sodium/hydrogen exchangerPRO_0000052380Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi420 – 4201N-linked (GlcNAc...)Sequence analysis
Modified residuei490 – 4901PhosphothreonineCombined sources
Modified residuei494 – 4941PhosphoserineCombined sources
Modified residuei498 – 4981PhosphothreonineCombined sources
Modified residuei499 – 4991PhosphoserineCombined sources
Glycosylationi515 – 5151N-linked (GlcNAc...)1 Publication
Glycosylationi550 – 5501N-linked (GlcNAc...)1 Publication
Glycosylationi563 – 5631N-linked (GlcNAc...)1 Publication
Modified residuei569 – 5691PhosphoserineCombined sources

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ04121.
TopDownProteomicsiQ04121.

PTM databases

iPTMnetiQ04121.

Interactioni

Subunit structurei

Interacts with CYP6.1 Publication

Protein-protein interaction databases

BioGridi32510. 167 interactions.
DIPiDIP-4145N.
IntActiQ04121. 16 interactions.
MINTiMINT-494386.

Structurei

3D structure databases

ProteinModelPortaliQ04121.
SMRiQ04121. Positions 57-418.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi124 – 13310Amiloride-binding

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi23 – 5028Asp-richAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00760000119074.
HOGENOMiHOG000172307.
InParanoidiQ04121.
KOiK12041.
OMAiFWHATDS.
OrthoDBiEOG092C1Y19.

Family and domain databases

InterProiIPR006153. Cation/H_exchanger.
IPR018422. Cation/H_exchanger_CPA1.
IPR004709. NaH_exchanger.
[Graphical view]
PANTHERiPTHR10110. PTHR10110. 3 hits.
PfamiPF00999. Na_H_Exchanger. 1 hit.
[Graphical view]
PRINTSiPR01084. NAHEXCHNGR.
TIGRFAMsiTIGR00840. b_cpa1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q04121-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSKVLLNIA FKVLLTTAKR AVDPDDDDEL LPSPDLPGSD DPIAGDPDVD
60 70 80 90 100
LNPVTEEMFS SWALFIMLLL LISALWSSYY LTQKRIRAVH ETVLSIFYGM
110 120 130 140 150
VIGLIIRMSP GHYIQDTVTF NSSYFFNVLL PPIILNSGYE LNQVNFFNNM
160 170 180 190 200
LSILIFAIPG TFISAVVIGI ILYIWTFLGL ESIDISFADA MSVGATLSAT
210 220 230 240 250
DPVTILSIFN AYKVDPKLYT IIFGESLLND AISIVMFETC QKFHGQPATF
260 270 280 290 300
SSVFEGAGLF LMTFSVSLLI GVLIGILVAL LLKHTHIRRY PQIESCLILL
310 320 330 340 350
IAYESYFFSN GCHMSGIVSL LFCGITLKHY AYYNMSRRSQ ITIKYIFQLL
360 370 380 390 400
ARLSENFIFI YLGLELFTEV ELVYKPLLII VAAISICVAR WCAVFPLSQF
410 420 430 440 450
VNWIYRVKTI RSMSGITGEN ISVPDEIPYN YQMMTFWAGL RGAVGVALAL
460 470 480 490 500
GIQGEYKFTL LATVLVVVVL TVIIFGGTTA GMLEVLNIKT GCISEEDTSD
510 520 530 540 550
DEFDIEAPRA INLLNGSSIQ TDLGPYSDNN SPDISIDQFA VSSNKNLPNN
560 570 580 590 600
ISTTGGNTFG GLNETENTSP NPARSSMDKR NLRDKLGTIF NSDSQWFQNF
610 620 630
DEQVLKPVFL DNVSPSLQDS ATQSPADFSS QNH
Length:633
Mass (Da):70,148
Last modified:November 1, 1996 - v1
Checksum:i9B771ABDE41CEB0A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33007 Genomic DNA. Translation: AAB64861.1.
BK006938 Genomic DNA. Translation: DAA12290.1.
PIRiS69734.
RefSeqiNP_010744.3. NM_001180764.3.

Genome annotation databases

EnsemblFungiiYDR456W; YDR456W; YDR456W.
GeneIDi852066.
KEGGisce:YDR456W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33007 Genomic DNA. Translation: AAB64861.1.
BK006938 Genomic DNA. Translation: DAA12290.1.
PIRiS69734.
RefSeqiNP_010744.3. NM_001180764.3.

3D structure databases

ProteinModelPortaliQ04121.
SMRiQ04121. Positions 57-418.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32510. 167 interactions.
DIPiDIP-4145N.
IntActiQ04121. 16 interactions.
MINTiMINT-494386.

Protein family/group databases

TCDBi2.A.36.1.12. the monovalent cation:proton antiporter-1 (cpa1) family.

PTM databases

iPTMnetiQ04121.

Proteomic databases

MaxQBiQ04121.
TopDownProteomicsiQ04121.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR456W; YDR456W; YDR456W.
GeneIDi852066.
KEGGisce:YDR456W.

Organism-specific databases

EuPathDBiFungiDB:YDR456W.
SGDiS000002864. NHX1.

Phylogenomic databases

GeneTreeiENSGT00760000119074.
HOGENOMiHOG000172307.
InParanoidiQ04121.
KOiK12041.
OMAiFWHATDS.
OrthoDBiEOG092C1Y19.

Enzyme and pathway databases

BioCyciYEAST:G3O-29984-MONOMER.
ReactomeiR-SCE-425986. Sodium/Proton exchangers.

Miscellaneous databases

PROiQ04121.

Family and domain databases

InterProiIPR006153. Cation/H_exchanger.
IPR018422. Cation/H_exchanger_CPA1.
IPR004709. NaH_exchanger.
[Graphical view]
PANTHERiPTHR10110. PTHR10110. 3 hits.
PfamiPF00999. Na_H_Exchanger. 1 hit.
[Graphical view]
PRINTSiPR01084. NAHEXCHNGR.
TIGRFAMsiTIGR00840. b_cpa1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNHX1_YEAST
AccessioniPrimary (citable) accession number: Q04121
Secondary accession number(s): D6VT80
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 149 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 521 molecules/cell in log phase SD medium.1 Publication

Caution

Numerous studies suggest that the C-terminal tail of the Na+/H+ exchangers assumes a cytosolic orientation and constitutes a regulatory region. This cytosolic localization is confirmed by phosphorylation analysis (PubMed:15665377 and PubMed:18407956). However, residues Asn-515, Asn-550 and Asn-563 have been shown to be glycosylated and localized at the lumenal side (PubMed:11036065). These contradictory results suggest an unusual topology of the C-terminal tail which may contain membrane spans formed by beta-sheets or even may switch from one side to the other of the membrane.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.