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Protein
Submitted name:

Pheromone cCF10 receptor

Gene

prgX

Organism
Enterococcus faecalis (Streptococcus faecalis)
Status
Unreviewed-Annotation score: Annotation score: 1 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

GO - Biological processi

  • negative regulation of transcription, DNA-templated Source: CACAO
Complete GO annotation...

Keywords - Molecular functioni

ReceptorImported

Names & Taxonomyi

Protein namesi
Submitted name:
Pheromone cCF10 receptorImported
Submitted name:
PrgXImported
Gene namesi
Name:prgXImported
Encoded oniPlasmid pCF10Imported
Plasmid pMG2200Imported
OrganismiEnterococcus faecalis (Streptococcus faecalis)Imported
Taxonomic identifieri1351 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus

Subcellular locationi

GO - Cellular componenti

  • transcriptional repressor complex Source: CACAO
Complete GO annotation...

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AW6X-ray3.00A/B1-317[»]
2AWIX-ray2.25A/B/C/D/E/F/G/H/I/J/K/L1-317[»]
2AXUX-ray2.90A/B/C/D/E/F/G/H/I/J/K/L1-317[»]
2AXVX-ray3.00A/B/C/D1-317[»]
2AXZX-ray3.00A/B/C/D1-317[»]
2GRLX-ray3.00A/B/C/D1-317[»]
SMRiQ04114. Positions 1-306.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 6155HTH cro/C1-type DNA-bindingInterPro annotationAdd
BLAST

Family and domain databases

Gene3Di1.10.260.40. 1 hit.
InterProiIPR001387. Cro/C1-type_HTH.
IPR010982. Lambda_DNA-bd_dom.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF01381. HTH_3. 1 hit.
[Graphical view]
SMARTiSM00530. HTH_XRE. 1 hit.
[Graphical view]
SUPFAMiSSF47413. SSF47413. 1 hit.
SSF48452. SSF48452. 1 hit.

Sequencei

Sequence statusi: Complete.

Q04114-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKIGSVLKQ IRQELNYHQI DLYSGIMSKS VYIKVEADSR PISVEELSKF
60 70 80 90 100
SERLGVNFFE ILNRAGMNTK SVNETGKEKL LISKIFTNPD LFDKNFQRIE
110 120 130 140 150
PKRLTSLQYF SIYLGYISIA HHYNIEVPTF NKTITSDLKH LYDKRTTFFG
160 170 180 190 200
IDYEIVSNLL NVLPYEEVSS IIKPMYPIVD SFGKDYDLTI QTVLKNALTI
210 220 230 240 250
SIMNRNLKEA QYYINQFEHL KTIKNISING YYDLEINYLK QIYQFLTDKN
260 270 280 290 300
IDSYLNAVNI INIFKIIGKE DIHRSLVEEL TKISAKEKFT PPKEVTMYYE
310
NYVAIENNPI PEIKEQS
Length:317
Mass (Da):37,094
Last modified:November 1, 1996 - v1
Checksum:i411A62B678164A1D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY855841 Genomic DNA. Translation: AAA65845.1.
AB374546 Genomic DNA. Translation: BAH02409.1.
PIRiB41662.
RefSeqiWP_002366018.1. NZ_JWBU01000151.1.
YP_002333461.1. NC_011642.1.
YP_195768.1. NC_006827.2.

Genome annotation databases

GeneIDi3267197.
7072416.
KEGGipg:3267197.
pg:7072416.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY855841 Genomic DNA. Translation: AAA65845.1.
AB374546 Genomic DNA. Translation: BAH02409.1.
PIRiB41662.
RefSeqiWP_002366018.1. NZ_JWBU01000151.1.
YP_002333461.1. NC_011642.1.
YP_195768.1. NC_006827.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AW6X-ray3.00A/B1-317[»]
2AWIX-ray2.25A/B/C/D/E/F/G/H/I/J/K/L1-317[»]
2AXUX-ray2.90A/B/C/D/E/F/G/H/I/J/K/L1-317[»]
2AXVX-ray3.00A/B/C/D1-317[»]
2AXZX-ray3.00A/B/C/D1-317[»]
2GRLX-ray3.00A/B/C/D1-317[»]
SMRiQ04114. Positions 1-306.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi3267197.
7072416.
KEGGipg:3267197.
pg:7072416.

Family and domain databases

Gene3Di1.10.260.40. 1 hit.
InterProiIPR001387. Cro/C1-type_HTH.
IPR010982. Lambda_DNA-bd_dom.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF01381. HTH_3. 1 hit.
[Graphical view]
SMARTiSM00530. HTH_XRE. 1 hit.
[Graphical view]
SUPFAMiSSF47413. SSF47413. 1 hit.
SSF48452. SSF48452. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Molecular and genetic analysis of a region of plasmid pCF10 containing positive control genes and structural genes encoding surface proteins involved in pheromone-inducible conjugation in Enterococcus faecalis."
    Kao S.M., Olmsted S.B., Viksnins A.S., Gallo J.C., Dunny G.M.
    J. Bacteriol. 173:7650-7664(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Plasmid: pCF10
  2. "Cloning and characterization of a region of the Enterococcus faecalis conjugative plasmid, pCF10, encoding a sex pheromone-binding function."
    Ruhfel R.E., Manias D.A., Dunny G.M.
    J. Bacteriol. 175:5253-5259(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Plasmid: pCF10
  3. "The prgQ gene of the Enterococcus faecalis tetracycline resistance plasmid pCF10 encodes a peptide inhibitor, iCF10."
    Nakayama J., Ruhfel R.E., Dunny G.M., Isogai A., Suzuki A.
    J. Bacteriol. 176:7405-7408(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Plasmid: pCF10
  4. "Identification and characterization of the genes of Enterococcus faecalis plasmid pCF10 involved in replication and in negative control of pheromone-inducible conjugation."
    Hedberg P.J., Leonard B.A., Ruhfel R.E., Dunny G.M.
    Plasmid 35:46-57(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Plasmid: pCF10
  5. "Characterization of the pheromone response of the Enterococcus faecalis conjugative plasmid pCF10: complete sequence and comparative analysis of the transcriptional and phenotypic responses of pCF10-containing cells to pheromone induction."
    Hirt H., Manias D.A., Bryan E.M., Klein J.R., Marklund J.K., Staddon J.H., Paustian M.L., Kapur V., Dunny G.M.
    J. Bacteriol. 187:1044-1054(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Plasmid: pCF10
  6. "Structure of peptide sex pheromone receptor PrgX and PrgX/pheromone complexes and regulation of conjugation in Enterococcus faecalis."
    Shi K., Brown C.K., Gu Z.Y., Kozlowicz B.K., Dunny G.M., Ohlendorf D.H., Earhart C.A.
    Proc. Natl. Acad. Sci. U.S.A. 102:18596-18601(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS).
  7. "Molecular basis for control of conjugation by bacterial pheromone and inhibitor peptides."
    Kozlowicz B.K., Shi K., Gu Z.Y., Ohlendorf D.H., Earhart C.A., Dunny G.M.
    Mol. Microbiol. 62:958-969(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.00 ANGSTROMS).
  8. "Isolation of VanB-type Enterococcus faecalis strains from nosocomial infections: first report of the isolation and identification of the pheromone-responsive plasmids pMG2200, Encoding VanB-type vancomycin resistance and a Bac41-type bacteriocin, and pMG2201, encoding erythromycin resistance and cytolysin (Hly/Bac)."
    Zheng B., Tomita H., Inoue T., Ike Y.
    Antimicrob. Agents Chemother. 53:735-747(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Plasmid: pMG2200

Entry informationi

Entry nameiQ04114_ENTFL
AccessioniPrimary (citable) accession number: Q04114
Entry historyi
Integrated into UniProtKB/TrEMBL: November 1, 1996
Last sequence update: November 1, 1996
Last modified: April 13, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, PlasmidImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.