Q040B2 (ARGB_LEUMM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetylglutamate kinase
EC=2.7.2.8

Alternative name(s):
N-acetyl-L-glutamate 5-phosphotransferase
NAG kinase
Short name=AGK

Gene names

Name:	argB
Ordered Locus Names:	LEUM_0058

Organism

Leuconostoc mesenteroides subsp. mesenteroides (strain ATCC 8293 / NCDO 523) [Complete proteome] [HAMAP]

Taxonomic identifier

203120 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Leuconostoc

Protein attributes

Sequence length	244 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate. HAMAP MF_00082_B
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4. HAMAP MF_00082_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00082_B.
Sequence similarities	Belongs to the acetylglutamate kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetylglutamate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 244	244	Acetylglutamate kinase HAMAP MF_00082_B		PRO_0000335642
Regions
	Region	40 – 41	2	Substrate binding By similarity
Sites
	Binding site	62	1	Substrate By similarity
	Binding site	155	1	Substrate By similarity
	Site	7	1	Transition state stabilizer By similarity
	Site	214	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q040B2 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: 31E4F64CCAD76745	FASTA	244	26,523
	10 20 30 40 50 60 MKKIVIKIGG NAATQLTPAF FDTIKFWQKQ HYKIAIVHGG GDTISALMSQ LNEPVQKING 70 80 90 100 110 120 IRFTTQNGIN ITKMALLGQV QPALLESCRV NGLSVIGLNA ASDQLLTGHT IDQDTFGYVG 130 140 150 160 170 180 NIHQVNTKLI HNLWEKNLIP IIAPMAITNS GQWLNVNADH AATALAKYLK ADELYLLTDV 190 200 210 220 230 240 SGVKIKGNVL QNLTSKTMEE LQKKQMITGG MIPKVNSALF AARHGVRAVH ITNTVTHPGT IVTI

References

[1]

"Comparative genomics of the lactic acid bacteria." Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V., Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V., Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M., Hawkins T. , Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A., Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W., Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y., Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R., O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T., Richardson P., Kozyavkin S., Weimer B.C., Mills D.A. Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006) [PubMed: 17030793] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8293 / NCDO 523.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000414 Genomic DNA. Translation: ABJ61210.1.
RefSeq	YP_817583.1. NC_008531.1.
3D structure databases
ProteinModelPortal	Q040B2.
ModBase	Search...
Protein-protein interaction databases
STRING	Q040B2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4422011.
GenomeReviews	Gene locus LEUM_0058 in contig CP000414_GR.
KEGG	lme:LEUM_0058.
PATRIC	22408101. VBILeuMes50664_0089.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0548.
HOGENOM	HBG497643.
OMA	GGNAMID.
PhylomeDB	Q040B2.
ProtClustDB	PRK00942.
Enzyme and pathway databases
BioCyc	LMES203120:LEUM_0058-MONOMER.
Family and domain databases
HAMAP	MF_00082_B. ArgB_B. [Tree]
InterPro	IPR004662. AcgluKinase. IPR001048. Asp/Glu/Uridylate_kinase. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
KO	K00930.
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000728. NAGK. 1 hit.
SUPFAM	SSF53633. Aa_kinase. 1 hit.
TIGRFAMs	TIGR00761. ArgB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARGB_LEUMM

Accession

Primary (citable) accession number: Q040B2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	November 14, 2006
Last modified:	January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program