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Q04087 (LRS4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Monopolin complex subunit LRS4
Alternative name(s):
Loss of rDNA silencing protein 4
Gene names
Name:LRS4
Ordered Locus Names:YDR439W
ORF Names:D9461.25
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length347 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the monopolin complex which promotes monoorientation during meiosis I, required for chromosome segregation during meiosis. Involved in rDNA silencing. Ref.3 Ref.4 Ref.5

Subunit structure

Component of the monopolin complex composed of at least CSM1, LRS4 and MAM1. The complex associates with the kinetochore. Ref.4

Subcellular location

Nucleusnucleolus. Chromosomecentromere. Note: Transiently released from the nucleolus and localized to the centromere regions during late pachytene. This relocation is CDC5 dependent. Ref.4 Ref.6 Ref.8 Ref.9

Post-translational modification

Phosphorylated by CDC5. This phosphorylation is required for the location to the kinetochores during late pachytene. Ref.10

Miscellaneous

Present with 1390 molecules/cell in log phase SD medium.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 347347Monopolin complex subunit LRS4
PRO_0000257808

Regions

Coiled coil46 – 11873 Potential

Amino acid modifications

Modified residue1681Phosphoserine Ref.11
Modified residue2301Phosphoserine Ref.12

Sequences

Sequence LengthMass (Da)Tools
Q04087 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 60D880BA947B9005

FASTA34739,354
        10         20         30         40         50         60 
MTTLLQLLSN YYKAKLDSER IYNEYVQSQY EFASLDKLNN NKGDPKKVVD ETLFLQRQIA 

        70         80         90        100        110        120 
QLNKQLQLSF QENEKLLSVQ KNQKALYQSK LSSKDAFIDD LKLKLKVEQI SVDKHNKERT 

       130        140        150        160        170        180 
PSTGRDEQQR NSKAAHTSKP TIHLLSPIVN RDKPNNQTND RGGNDPDSPT SQRRSRGLRS 

       190        200        210        220        230        240 
LLSSGKNTIF DSISKNLDDE INENAHIRND TTSSKIAGKS PSRLSALQKS PELRKERNNM 

       250        260        270        280        290        300 
ILKEHILRSK DDQNITSSRK LDNIELSSIG DSTAMTSRSS TVNANDILGN EENDGITKLK 

       310        320        330        340 
RVNKLTSSPV KRDCSTNKKR KLTKQRIATL PNSDEELSNN LNVDEFV 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"A genetic screen for ribosomal DNA silencing defects identifies multiple DNA replication and chromatin-modulating factors."
Smith J.S., Caputo E., Boeke J.D.
Mol. Cell. Biol. 19:3184-3197(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"Kinetochore recruitment of two nucleolar proteins is required for homolog segregation in meiosis I."
Rabitsch K.P., Petronczki M., Javerzat J.-P., Genier S., Chwalla B., Schleiffer A., Tanaka T.U., Nasmyth K.
Dev. Cell 4:535-548(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION IN THE MONOPOLIN COMPLEX.
[5]"Large-scale functional genomic analysis of sporulation and meiosis in Saccharomyces cerevisiae."
Enyenihi A.H., Saunders W.S.
Genetics 163:47-54(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Polo-like kinase Cdc5 promotes chiasmata formation and cosegregation of sister centromeres at meiosis I."
Clyne R.K., Katis V.L., Jessop L., Benjamin K.R., Herskowitz I., Lichten M., Nasmyth K.
Nat. Cell Biol. 5:480-485(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[9]"Spo13 maintains centromeric cohesion and kinetochore coorientation during meiosis I."
Lee B.H., Kiburz B.M., Amon A.
Curr. Biol. 14:2168-2182(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, ASSOCIATION TO THE KINETOCHORE.
[10]"Spo13 facilitates monopolin recruitment to kinetochores and regulates maintenance of centromeric cohesion during yeast meiosis."
Katis V.L., Matos J., Mori S., Shirahige K., Zachariae W., Nasmyth K.
Curr. Biol. 14:2183-2196(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION.
[11]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-168, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-230, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U33007 Genomic DNA. Translation: AAB64867.1.
BK006938 Genomic DNA. Translation: DAA12276.1.
PIRS69719.
RefSeqNP_010727.1. NM_001180747.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3N7NX-ray3.90E/F1-102[»]
ProteinModelPortalQ04087.
SMRQ04087. Positions 3-33.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32495. 359 interactions.
DIPDIP-1416N.
IntActQ04087. 14 interactions.
MINTMINT-391498.
STRING4932.YDR439W.

Proteomic databases

PaxDbQ04087.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDR439W; YDR439W; YDR439W.
GeneID852049.
KEGGsce:YDR439W.

Organism-specific databases

CYGDYDR439w.
SGDS000002847. LRS4.

Phylogenomic databases

eggNOGNOG72052.
KOK12775.
OMAMAHENER.
OrthoDBEOG7673QD.

Enzyme and pathway databases

BioCycYEAST:G3O-29973-MONOMER.

Gene expression databases

GenevestigatorQ04087.

Family and domain databases

InterProIPR018479. Monopolin_cplx_su-Lrs4.
[Graphical view]
PfamPF10422. LRS4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ04087.
NextBio970308.

Entry information

Entry nameLRS4_YEAST
AccessionPrimary (citable) accession number: Q04087
Secondary accession number(s): D6VT66
Entry history
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

PDB cross-references

Index of Protein Data Bank (PDB) cross-references