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Q04087

- LRS4_YEAST

UniProt

Q04087 - LRS4_YEAST

Protein

Monopolin complex subunit LRS4

Gene

LRS4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Component of the monopolin complex which promotes monoorientation during meiosis I, required for chromosome segregation during meiosis. Involved in rDNA silencing.3 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. chromatin silencing at rDNA Source: SGD
    2. homologous chromosome segregation Source: SGD
    3. protein localization to nucleolar rDNA repeats Source: SGD
    4. rDNA condensation Source: SGD

    Keywords - Biological processi

    Cell cycle, Meiosis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29973-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Monopolin complex subunit LRS4
    Alternative name(s):
    Loss of rDNA silencing protein 4
    Gene namesi
    Name:LRS4
    Ordered Locus Names:YDR439W
    ORF Names:D9461.25
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDR439w.
    SGDiS000002847. LRS4.

    Subcellular locationi

    Nucleusnucleolus. Chromosomecentromere
    Note: Transiently released from the nucleolus and localized to the centromere regions during late pachytene. This relocation is CDC5 dependent.

    GO - Cellular componenti

    1. monopolin complex Source: SGD
    2. nucleolus Source: SGD
    3. nucleus Source: SGD

    Keywords - Cellular componenti

    Centromere, Chromosome, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 347347Monopolin complex subunit LRS4PRO_0000257808Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei168 – 1681Phosphoserine2 Publications
    Modified residuei230 – 2301Phosphoserine2 Publications

    Post-translational modificationi

    Phosphorylated by CDC5. This phosphorylation is required for the location to the kinetochores during late pachytene.3 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ04087.
    PaxDbiQ04087.

    Expressioni

    Gene expression databases

    GenevestigatoriQ04087.

    Interactioni

    Subunit structurei

    Component of the monopolin complex composed of at least CSM1, LRS4 and MAM1. The complex associates with the kinetochore.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CDC5P325626EBI-32189,EBI-4440
    CDC7P062435EBI-32189,EBI-4451
    CSM1P2565116EBI-32189,EBI-22001

    Protein-protein interaction databases

    BioGridi32495. 359 interactions.
    DIPiDIP-1416N.
    IntActiQ04087. 14 interactions.
    MINTiMINT-391498.
    STRINGi4932.YDR439W.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3N7NX-ray3.90E/F1-102[»]
    ProteinModelPortaliQ04087.
    SMRiQ04087. Positions 3-33.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ04087.

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili46 – 11873Sequence AnalysisAdd
    BLAST

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG72052.
    KOiK12775.
    OMAiINENAHI.
    OrthoDBiEOG7673QD.

    Family and domain databases

    InterProiIPR018479. Monopolin_cplx_su-Lrs4.
    [Graphical view]
    PfamiPF10422. LRS4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q04087-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTTLLQLLSN YYKAKLDSER IYNEYVQSQY EFASLDKLNN NKGDPKKVVD    50
    ETLFLQRQIA QLNKQLQLSF QENEKLLSVQ KNQKALYQSK LSSKDAFIDD 100
    LKLKLKVEQI SVDKHNKERT PSTGRDEQQR NSKAAHTSKP TIHLLSPIVN 150
    RDKPNNQTND RGGNDPDSPT SQRRSRGLRS LLSSGKNTIF DSISKNLDDE 200
    INENAHIRND TTSSKIAGKS PSRLSALQKS PELRKERNNM ILKEHILRSK 250
    DDQNITSSRK LDNIELSSIG DSTAMTSRSS TVNANDILGN EENDGITKLK 300
    RVNKLTSSPV KRDCSTNKKR KLTKQRIATL PNSDEELSNN LNVDEFV 347
    Length:347
    Mass (Da):39,354
    Last modified:November 1, 1996 - v1
    Checksum:i60D880BA947B9005
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U33007 Genomic DNA. Translation: AAB64867.1.
    BK006938 Genomic DNA. Translation: DAA12276.1.
    PIRiS69719.
    RefSeqiNP_010727.1. NM_001180747.1.

    Genome annotation databases

    EnsemblFungiiYDR439W; YDR439W; YDR439W.
    GeneIDi852049.
    KEGGisce:YDR439W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U33007 Genomic DNA. Translation: AAB64867.1 .
    BK006938 Genomic DNA. Translation: DAA12276.1 .
    PIRi S69719.
    RefSeqi NP_010727.1. NM_001180747.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3N7N X-ray 3.90 E/F 1-102 [» ]
    ProteinModelPortali Q04087.
    SMRi Q04087. Positions 3-33.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32495. 359 interactions.
    DIPi DIP-1416N.
    IntActi Q04087. 14 interactions.
    MINTi MINT-391498.
    STRINGi 4932.YDR439W.

    Proteomic databases

    MaxQBi Q04087.
    PaxDbi Q04087.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDR439W ; YDR439W ; YDR439W .
    GeneIDi 852049.
    KEGGi sce:YDR439W.

    Organism-specific databases

    CYGDi YDR439w.
    SGDi S000002847. LRS4.

    Phylogenomic databases

    eggNOGi NOG72052.
    KOi K12775.
    OMAi INENAHI.
    OrthoDBi EOG7673QD.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29973-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q04087.
    NextBioi 970308.

    Gene expression databases

    Genevestigatori Q04087.

    Family and domain databases

    InterProi IPR018479. Monopolin_cplx_su-Lrs4.
    [Graphical view ]
    Pfami PF10422. LRS4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "A genetic screen for ribosomal DNA silencing defects identifies multiple DNA replication and chromatin-modulating factors."
      Smith J.S., Caputo E., Boeke J.D.
      Mol. Cell. Biol. 19:3184-3197(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. "Kinetochore recruitment of two nucleolar proteins is required for homolog segregation in meiosis I."
      Rabitsch K.P., Petronczki M., Javerzat J.-P., Genier S., Chwalla B., Schleiffer A., Tanaka T.U., Nasmyth K.
      Dev. Cell 4:535-548(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION IN THE MONOPOLIN COMPLEX.
    5. "Large-scale functional genomic analysis of sporulation and meiosis in Saccharomyces cerevisiae."
      Enyenihi A.H., Saunders W.S.
      Genetics 163:47-54(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Polo-like kinase Cdc5 promotes chiasmata formation and cosegregation of sister centromeres at meiosis I."
      Clyne R.K., Katis V.L., Jessop L., Benjamin K.R., Herskowitz I., Lichten M., Nasmyth K.
      Nat. Cell Biol. 5:480-485(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    9. "Spo13 maintains centromeric cohesion and kinetochore coorientation during meiosis I."
      Lee B.H., Kiburz B.M., Amon A.
      Curr. Biol. 14:2168-2182(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, ASSOCIATION TO THE KINETOCHORE.
    10. "Spo13 facilitates monopolin recruitment to kinetochores and regulates maintenance of centromeric cohesion during yeast meiosis."
      Katis V.L., Matos J., Mori S., Shirahige K., Zachariae W., Nasmyth K.
      Curr. Biol. 14:2183-2196(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION.
    11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-168, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-230, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiLRS4_YEAST
    AccessioniPrimary (citable) accession number: Q04087
    Secondary accession number(s): D6VT66
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 31, 2006
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1390 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3