Monopolin complex subunit LRS4 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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Q04087 (LRS4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 85. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Monopolin complex subunit LRS4

Alternative name(s):
Loss of rDNA silencing protein 4

Gene names

Name:	LRS4
Ordered Locus Names:	YDR439W
ORF Names:	D9461.25

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the monopolin complex which promotes monoorientation during meiosis I, required for chromosome segregation during meiosis. Involved in rDNA silencing. Ref.3 Ref.4 Ref.5
Subunit structure	Component of the monopolin complex composed of at least CSM1, LRS4 and MAM1. The complex associates with the kinetochore. Ref.4
Subcellular location	Nucleus › nucleolus. Chromosome › centromere. Note: Transiently released from the nucleolus and localized to the centromere regions during late pachytene. This relocation is CDC5 dependent. Ref.4 Ref.6 Ref.8 Ref.9
Post-translational modification	Phosphorylated by CDC5. This phosphorylation is required for the location to the kinetochores during late pachytene. Ref.10
Miscellaneous	Present with 1390 molecules/cell in log phase SD medium.

Ontologies

Keywords
Biological process	Cell cycle Meiosis
Cellular component	Centromere Chromosome Nucleus
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	chromatin silencing at rDNA Inferred from direct assay Ref.3. Source: SGD homologous chromosome segregation Inferred from physical interaction Ref.4. Source: SGD protein localization to nucleolar rDNA repeats Inferred from mutant phenotype PubMed 19362534. Source: SGD rDNA condensation Inferred from mutant phenotype PubMed 18923139. Source: SGD
Cellular_component	monopolin complex Inferred from direct assay Ref.4. Source: SGD nucleolus Inferred from direct assay Ref.4. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
CDC5	P32562	6	EBI-32189,EBI-4440
CDC7	P06243	5	EBI-32189,EBI-4451
CSM1	P25651	16	EBI-32189,EBI-22001

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 347

347

Monopolin complex subunit LRS4

PRO_0000257808

Regions

Coiled coil

46 – 118

Potential

Amino acid modifications

Modified residue

146

Phosphoserine Ref.12

Modified residue

168

Phosphoserine Ref.11 Ref.12

Modified residue

230

Phosphoserine Ref.11 Ref.12

Sequences

Sequence

Length

Mass (Da)

Tools

Q04087 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 60D880BA947B9005
Show »

FASTA

347

39,354

        10         20         30         40         50         60 
MTTLLQLLSN YYKAKLDSER IYNEYVQSQY EFASLDKLNN NKGDPKKVVD ETLFLQRQIA 

        70         80         90        100        110        120 
QLNKQLQLSF QENEKLLSVQ KNQKALYQSK LSSKDAFIDD LKLKLKVEQI SVDKHNKERT 

       130        140        150        160        170        180 
PSTGRDEQQR NSKAAHTSKP TIHLLSPIVN RDKPNNQTND RGGNDPDSPT SQRRSRGLRS 

       190        200        210        220        230        240 
LLSSGKNTIF DSISKNLDDE INENAHIRND TTSSKIAGKS PSRLSALQKS PELRKERNNM 

       250        260        270        280        290        300 
ILKEHILRSK DDQNITSSRK LDNIELSSIG DSTAMTSRSS TVNANDILGN EENDGITKLK 

       310        320        330        340 
RVNKLTSSPV KRDCSTNKKR KLTKQRIATL PNSDEELSNN LNVDEFV

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. Zaccaria P. Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"A genetic screen for ribosomal DNA silencing defects identifies multiple DNA replication and chromatin-modulating factors." Smith J.S., Caputo E., Boeke J.D. Mol. Cell. Biol. 19:3184-3197(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[4]	"Kinetochore recruitment of two nucleolar proteins is required for homolog segregation in meiosis I." Rabitsch K.P., Petronczki M., Javerzat J.-P., Genier S., Chwalla B., Schleiffer A., Tanaka T.U., Nasmyth K. Dev. Cell 4:535-548(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION IN THE MONOPOLIN COMPLEX.
[5]	"Large-scale functional genomic analysis of sporulation and meiosis in Saccharomyces cerevisiae." Enyenihi A.H., Saunders W.S. Genetics 163:47-54(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Polo-like kinase Cdc5 promotes chiasmata formation and cosegregation of sister centromeres at meiosis I." Clyne R.K., Katis V.L., Jessop L., Benjamin K.R., Herskowitz I., Lichten M., Nasmyth K. Nat. Cell Biol. 5:480-485(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[9]	"Spo13 maintains centromeric cohesion and kinetochore coorientation during meiosis I." Lee B.H., Kiburz B.M., Amon A. Curr. Biol. 14:2168-2182(2004) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, ASSOCIATION TO THE KINETOCHORE.
[10]	"Spo13 facilitates monopolin recruitment to kinetochores and regulates maintenance of centromeric cohesion during yeast meiosis." Katis V.L., Matos J., Mori S., Shirahige K., Zachariae W., Nasmyth K. Curr. Biol. 14:2183-2196(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION.
[11]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-168 AND SER-230, MASS SPECTROMETRY.
[12]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-146; SER-168 AND SER-230, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U33007 Genomic DNA. Translation: AAB64867.1.
BK006938 Genomic DNA. Translation: DAA12276.1.

PIR

S69719.

RefSeq

NP_010727.1. NM_001180747.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3N7N	X-ray	3.90	E/F	1-102	[»]

ProteinModelPortal

Q04087.

SMR

Q04087. Positions 3-33.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-1416N.

IntAct

Q04087. 14 interactions.

MINT

MINT-391498.

STRING

4932.YDR439W.

Proteomic databases

PaxDb

Q04087.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YDR439W; YDR439W; YDR439W.

GeneID

852049.

KEGG

sce:YDR439W.

Organism-specific databases

CYGD

YDR439w.

SGD

S000002847. LRS4.

Phylogenomic databases

eggNOG

NOG72052.

K12775.

OMA

MAHENER.

OrthoDB

EOG4GXJXP.

Gene expression databases

Genevestigator

Q04087.

GermOnline

YDR439W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR018479. Monopolin_cplx_su-Lrs4.
[Graphical view]

Pfam

PF10422. LRS4. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q04087.

NextBio

970308.

Entry information

Entry name

LRS4_YEAST

Accession

Primary (citable) accession number: Q04087
Secondary accession number(s): D6VT66

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents