Reviewed, UniProtKB/Swiss-Prot Q04082 (GPI19_YEAST)

Names and origin

Protein names

Recommended name:
    Phosphatidylinositol N-acetylglucosaminyltransferase subunit GPI19
    EC=2.4.1.198

Gene names

Name:	GPI19
Ordered Locus Names:	YDR437W
ORF Names:	D9461.23

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	140 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Part of the complex catalyzing the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, the first step of GPI biosynthesis. Involved in cell wall biosynthesis. Ref.4
Catalytic activity	UDP-N-acetyl-D-glucosamine + 1-phosphatidyl-1D-myo-inositol = UDP + 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol.
Pathway	Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
Subunit structure	Component of the phosphatidylinositol N-acetylglucosaminyltransferase complex composed of at least GPI1, GPI2, GPI3, GPI15, GPI19 and ERI1.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Potential.
Miscellaneous	Present with 752 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Belongs to the GPI19 family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation GPI-anchor biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GPI anchor biosynthetic process Ref.4 Inferred from mutant phenotype. Source: SGD cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex Ref.4 Inferred from physical interaction. Source: SGD integral to endoplasmic reticulum membrane Ref.4 Inferred from direct assay. Source: SGD nuclear envelope Inferred from direct assay. Source: SGD
Molecular function	phosphatidylinositol N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC protein binding Ref.4 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GPI2	P46961	1	EBI-36611,EBI-7809

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 140	140	Phosphatidylinositol N-acetylglucosaminyltransferase subunit GPI19		PRO_0000240380
Regions
	Topological domain	1 – 12	12	Cytoplasmic Ref.4
	Transmembrane	13 – 33	21	Potential
	Topological domain	34 – 52	19	Lumenal Ref.4
	Transmembrane	53 – 73	21	Potential
	Topological domain	74 – 140	67	Cytoplasmic Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q04082-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: EFA63AAB82665B79	FASTA	140	16,090
	10 20 30 40 50 60 MYTKEYYWFS QYMIITSTLV LTIIWSILPS SLGEAAPKQF INTLLDIFPQ RRWIITLESI 70 80 90 100 110 120 MLMGMLCTYI GLLMYNEDTL TPPLDSLSTV TDAGGQLVIE DDPDVFVKKW AFKETSGIYD 130 140 LSLMDACQLL YLYDNDHTST

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"Gpi19, the Saccharomyces cerevisiae homologue of mammalian PIG-P, is a subunit of the initial enzyme for glycosylphosphatidylinositol anchor biosynthesis." Newman H.A., Romeo M.J., Lewis S.E., Yan B.C., Orlean P., Levin D.E. Eukaryot. Cell 4:1801-1807(2005) [PubMed: 16278447] [Abstract] Cited for: FUNCTION, TOPOLOGY, INTERACTION WITH GPI2, IDENTIFICATION IN THE GPI-GLCNAC TRANSFERASE COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U33007 Genomic DNA. Translation: AAB64854.1.
PIR	S69717.
RefSeq	NP_010725.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q04082. 19 interactions.
STRING	Q04082.
Genome annotation databases
Ensembl	YDR437W; YDR437W; YDR437W; Saccharomyces cerevisiae. [Genome view]
GeneID	852047.
GenomeReviews	Gene locus YDR437W in contig Z71256_GR.
KEGG	sce:YDR437W.
NMPDR	fig|4932.3.peg.1498.
Organism-specific databases
CYGD	YDR437w.
SGD	S000002845. GPI19.
Phylogenomic databases
HOGENOM	Q04082.
OMA	DLRTITD.
Enzyme and pathway databases
BRENDA	2.4.1.198. 250.
Gene expression databases
ArrayExpress	Q04082.
Genevestigator	Q04082.
GermOnline	YDR437W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013717. PIG-P. [Graphical view]
Pfam	PF08510. PIG-P. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	970302.

Entry information

Entry name

GPI19_YEAST

Accession

Primary (citable) accession number: Q04082

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	November 1, 1996
Last modified:	November 3, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)