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Protein

Leucine carboxyl methyltransferase 1

Gene

PPM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Methylates the carboxyl group of the C-terminal leucine residue of protein phosphatase 2A catalytic subunits to form alpha-leucine ester residues. Acts on the two major protein phosphatase 2A catalytic subunits, PPH21 and PPH22.2 Publications

Catalytic activityi

S-adenosyl-L-methionine + [phosphatase 2A protein]-leucine = S-adenosyl-L-homocysteine + [phosphatase 2A protein]-leucine methyl ester.

Enzyme regulationi

Inhibited by S-adenosyl-L-homocysteine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei81S-adenosyl-L-methionine1
Binding sitei105S-adenosyl-L-methionine; via carbonyl oxygen1
Binding sitei128S-adenosyl-L-methionine1
Binding sitei201S-adenosyl-L-methionine; via carbonyl oxygen1

GO - Molecular functioni

  • protein C-terminal leucine carboxyl O-methyltransferase activity Source: SGD

GO - Biological processi

  • cellular protein complex assembly Source: SGD
  • C-terminal protein methylation Source: SGD
  • regulation of autophagy Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciYEAST:YDR435C-MONOMER.
BRENDAi2.1.1.233. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine carboxyl methyltransferase 1 (EC:2.1.1.233)
Alternative name(s):
Protein phosphatase methyltransferase 1
[Phosphatase 2A protein]-leucine-carboxy methyltransferase 1
Gene namesi
Name:PPM1
Ordered Locus Names:YDR435C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR435C.
SGDiS000002843. PPM1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002261351 – 328Leucine carboxyl methyltransferase 1Add BLAST328

Proteomic databases

MaxQBiQ04081.
PRIDEiQ04081.

Interactioni

Protein-protein interaction databases

BioGridi32491. 95 interactors.
DIPiDIP-5435N.
MINTiMINT-517778.

Structurei

Secondary structure

1328
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 6Combined sources4
Helixi8 – 22Combined sources15
Turni28 – 32Combined sources5
Helixi35 – 55Combined sources21
Helixi57 – 68Combined sources12
Helixi72 – 95Combined sources24
Beta strandi97 – 104Combined sources8
Helixi112 – 118Combined sources7
Beta strandi122 – 128Combined sources7
Helixi130 – 142Combined sources13
Helixi144 – 150Combined sources7
Beta strandi162 – 165Combined sources4
Beta strandi167 – 173Combined sources7
Beta strandi176 – 178Combined sources3
Helixi179 – 187Combined sources9
Beta strandi192 – 194Combined sources3
Beta strandi196 – 202Combined sources7
Helixi204 – 206Combined sources3
Helixi209 – 222Combined sources14
Beta strandi224 – 233Combined sources10
Helixi243 – 255Combined sources13
Turni260 – 265Combined sources6
Helixi268 – 272Combined sources5
Helixi273 – 275Combined sources3
Beta strandi278 – 285Combined sources8
Helixi286 – 292Combined sources7
Helixi296 – 303Combined sources8
Helixi311 – 318Combined sources8
Beta strandi321 – 328Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RJDX-ray1.80A/B/C1-328[»]
1RJEX-ray2.00A/B/C1-328[»]
1RJFX-ray2.25A/B/C1-328[»]
1RJGX-ray2.61A1-328[»]
2OB1X-ray1.90A/B/C10-328[»]
2OB2X-ray1.92A/B/C2-328[»]
ProteinModelPortaliQ04081.
SMRiQ04081.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ04081.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni175 – 177S-adenosyl-L-methionine binding3

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000113293.
InParanoidiQ04081.
KOiK18203.
OMAiMNYGTYL.
OrthoDBiEOG092C4D3U.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR016651. Leu_CO_MeTrfase_LCMT1.
IPR007213. Ppm1/Ppm2/Tcmp.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR13600. PTHR13600. 2 hits.
PfamiPF04072. LCM. 1 hit.
[Graphical view]
PIRSFiPIRSF016305. LCM_mtfrase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

Q04081-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERIIQQTDY DALSCKLAAI SVGYLPSSGL QRLSVDLSKK YTEWHRSYLI
60 70 80 90 100
TLKKFSRRAF GKVDKAMRSS FPVMNYGTYL RTVGIDAAIL EFLVANEKVQ
110 120 130 140 150
VVNLGCGSDL RMLPLLQMFP HLAYVDIDYN ESVELKNSIL RESEILRISL
160 170 180 190 200
GLSKEDTAKS PFLIDQGRYK LAACDLNDIT ETTRLLDVCT KREIPTIVIS
210 220 230 240 250
ECLLCYMHNN ESQLLINTIM SKFSHGLWIS YDPIGGSQPN DRFGAIMQSN
260 270 280 290 300
LKESRNLEMP TLMTYNSKEK YASRWSAAPN VIVNDMWEIF NAQIPESERK
310 320
RLRSLQFLDE LEELKVMQTH YILMKAQW
Length:328
Mass (Da):37,695
Last modified:November 1, 1996 - v1
Checksum:i5FAB5B9F8B04D154
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33007 Genomic DNA. Translation: AAB64876.1.
BK006938 Genomic DNA. Translation: DAA12272.1.
PIRiS69715.
RefSeqiNP_010723.1. NM_001180743.1.

Genome annotation databases

EnsemblFungiiYDR435C; YDR435C; YDR435C.
GeneIDi852045.
KEGGisce:YDR435C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33007 Genomic DNA. Translation: AAB64876.1.
BK006938 Genomic DNA. Translation: DAA12272.1.
PIRiS69715.
RefSeqiNP_010723.1. NM_001180743.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RJDX-ray1.80A/B/C1-328[»]
1RJEX-ray2.00A/B/C1-328[»]
1RJFX-ray2.25A/B/C1-328[»]
1RJGX-ray2.61A1-328[»]
2OB1X-ray1.90A/B/C10-328[»]
2OB2X-ray1.92A/B/C2-328[»]
ProteinModelPortaliQ04081.
SMRiQ04081.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32491. 95 interactors.
DIPiDIP-5435N.
MINTiMINT-517778.

Proteomic databases

MaxQBiQ04081.
PRIDEiQ04081.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR435C; YDR435C; YDR435C.
GeneIDi852045.
KEGGisce:YDR435C.

Organism-specific databases

EuPathDBiFungiDB:YDR435C.
SGDiS000002843. PPM1.

Phylogenomic databases

HOGENOMiHOG000113293.
InParanoidiQ04081.
KOiK18203.
OMAiMNYGTYL.
OrthoDBiEOG092C4D3U.

Enzyme and pathway databases

BioCyciYEAST:YDR435C-MONOMER.
BRENDAi2.1.1.233. 984.

Miscellaneous databases

EvolutionaryTraceiQ04081.
PROiQ04081.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR016651. Leu_CO_MeTrfase_LCMT1.
IPR007213. Ppm1/Ppm2/Tcmp.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR13600. PTHR13600. 2 hits.
PfamiPF04072. LCM. 1 hit.
[Graphical view]
PIRSFiPIRSF016305. LCM_mtfrase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLCMT1_YEAST
AccessioniPrimary (citable) accession number: Q04081
Secondary accession number(s): D6VT62
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.