Q04062 (RPN9_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
26S proteasome regulatory subunit RPN9

Alternative name(s):
Proteasome non-ATPase subunit 7

Gene names

Name:	RPN9
Synonyms:	NAS7
Ordered Locus Names:	YDR427W
ORF Names:	D9461.14

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. Ref.4
Miscellaneous	Present with 12400 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the proteasome subunit S11 family. Contains 1 PCI domain.

Ontologies

Keywords
Cellular component	Proteasome
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	proteasome assembly Inferred from mutant phenotype. Source: SGD ubiquitin-dependent protein catabolic process Inferred from mutant phenotype. Source: SGD
Cellular component	proteasome regulatory particle, lid subcomplex Inferred from direct assay. Source: SGD proteasome storage granule Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct structural molecule activity Inferred from mutant phenotype. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RPN11	P43588	4	EBI-15944,EBI-11219
RPN5	Q12250	8	EBI-15944,EBI-15935
RPN6	Q12377	4	EBI-15944,EBI-308
RPN8	Q08723	6	EBI-15944,EBI-36176
RPT3	P33298	3	EBI-15944,EBI-13905

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 393	393	26S proteasome regulatory subunit RPN9		PRO_0000173870
Regions
	Domain	282 – 352	71	PCI

Sequences

	Sequence		Length	Mass (Da)
	Q04062 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 540F7DF8C23469A1	FASTA	393	45,782
	10 20 30 40 50 60 MFNNHEIDTI LSTLRMEADP SLHPLFEQFE KFYEEKLWFQ LSESLTKFFD DAKSTPLRLR 70 80 90 100 110 120 LYDNFVSKFY DKINQLSVVK YLLASLKDSK DFDESLKYLD DLKAQFQELD SKKQRNNGSK 130 140 150 160 170 180 DHGDGILLID SEIARTYLLK NDLVKARDLL DDLEKTLDKK DSIPLRITNS FYSTNSQYFK 190 200 210 220 230 240 FKNDFNSFYY TSLLYLSTLE PSTSITLAER QQLAYDLSIS ALLGDKIYNF GELLHHPIME 250 260 270 280 290 300 TIVNDSNYDW LFQLLNALTV GDFDKFDSLI KVQISKIPIL AQHESFLRQK ICLMTLIETV 310 320 330 340 350 360 FVKNIRMLSF EDISKATHLP KDNVEHLVMR AISLGLLKGS IDQVNELVTI SWVQPRIISG 370 380 390 DQITKMKDRL VEWNDQVEKL GKKMEARGQS IWV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"N-terminal modifications of the 19S regulatory particle subunits of the yeast proteasome." Kimura Y., Saeki Y., Yokosawa H., Polevoda B., Sherman F., Hirano H. Arch. Biochem. Biophys. 409:341-348(2003) [PubMed: 12504901] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-6.
[4]	"cDNA cloning and functional analysis of p28 (Nas6p) and p40.5 (Nas7p), two novel regulatory subunits of the 26S proteasome." Hori T., Kato S., Saeki M., DeMartino G.N., Slaughter C.A., Takeuchi J., Toh-e A., Tanaka K. Gene 216:113-122(1998) [PubMed: 9714768] [Abstract] Cited for: FUNCTION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U33007 Genomic DNA. Translation: AAB64853.1. BK006938 Genomic DNA. Translation: DAA12266.1.
PIR	S69708.
RefSeq	NP_010715.1. NM_001180735.1.
3D structure databases
ProteinModelPortal	Q04062.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5261N.
IntAct	Q04062. 51 interactions.
MINT	MINT-529103.
STRING	Q04062.
Proteomic databases
PeptideAtlas	Q04062.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YDR427W; YDR427W; YDR427W.
GeneID	852037.
KEGG	sce:YDR427W.
NMPDR	fig|4932.3.peg.1488.
Organism-specific databases
CYGD	YDR427w.
SGD	S000002835. RPN9.
Phylogenomic databases
eggNOG	fuNOG07264.
GeneTree	EFGT00050000006045.
HOGENOM	HBG396073.
OMA	LAHPILE.
OrthoDB	EOG4XSPZG.
Gene expression databases
ArrayExpress	Q04062.
Genevestigator	Q04062.
GermOnline	YDR427W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000717. PCI_dom. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K03039.
Pfam	PF01399. PCI. 1 hit. [Graphical view]
SMART	SM00088. PINT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	970278.

Entry information

Entry name

RPN9_YEAST

Accession

Primary (citable) accession number: Q04062
Secondary accession number(s): D6VT56

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2002
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program