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Protein

DNA polymerase eta

Gene

RAD30

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Plays an important role in the repair of UV-induced pyrimidine dimers. Depending on the context, it inserts the correct base, but causes frequent base transitions and transversions. Efficiently incorporates nucleotides opposite to other UV or oxidative DNA damages like O6-methylguanine, 7,8-dihydro-8-oxoguanine, 2,6-diamino-4-hydroxy-5-formamidopyrimidine of 2'-deoxyguanosine (FaPydG), or p-benzoquinone DNA adducts.34 Publications

Miscellaneous

Present with 1860 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi30MagnesiumPROSITE-ProRule annotation1
Metal bindingi155MagnesiumPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • chromosome segregation Source: SGD
  • DNA replication Source: UniProtKB-KW
  • error-free translesion synthesis Source: SGD
  • error-prone translesion synthesis Source: SGD
  • mitotic sister chromatid cohesion Source: SGD

Keywordsi

Molecular functionDNA-binding, DNA-directed DNA polymerase, Mutator protein, Nucleotidyltransferase, Transferase
Biological processDNA damage, DNA repair, DNA replication, DNA synthesis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29960-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase eta (EC:2.7.7.7)
Alternative name(s):
Radiation-sensitive protein 30
Gene namesi
Name:RAD30
Synonyms:DBH1
Ordered Locus Names:YDR419W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR419W.
SGDiS000002827. RAD30.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi30D → A: Abolishes DNA polymerase activity. 1 Publication1
Mutagenesisi34F → L: Alters translesion activity. 1 Publication1
Mutagenesisi39E → A: Abolishes DNA polymerase activity. 1 Publication1
Mutagenesisi64Y → F or A: Decreases efficiency of nucleotide incorporation. 1 Publication1
Mutagenesisi67R → A: Decreases efficiency of nucleotide incorporation. 1 Publication1
Mutagenesisi155D → A: Abolishes DNA polymerase activity and increases UV-induced mutations. 1 Publication1
Mutagenesisi156E → A: Decreases efficiency of nucleotide incorporation. 1 Publication1
Mutagenesisi279K → A: Decreases efficiency of nucleotide incorporation. 1 Publication1
Mutagenesisi627F → A: Abolishes POL30-binding; when associated with A-628. 1
Mutagenesisi628F → A: Abolishes POL30-binding; when associated with A-627. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002686981 – 632DNA polymerase etaAdd BLAST632

Proteomic databases

MaxQBiQ04049.
PRIDEiQ04049.

Expressioni

Inductioni

By UV radiation and heat shock. The mRNA is stabilized during stationary phase.2 Publications

Interactioni

Subunit structurei

Interacts with POL30. This interaction is essential for the polymerase eta function.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi32477. 135 interactors.
DIPiDIP-6500N.
IntActiQ04049. 21 interactors.
MINTiMINT-708283.
STRINGi4932.YDR419W.

Structurei

Secondary structure

1632
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi1 – 5Combined sources5
Helixi6 – 10Combined sources5
Helixi11 – 13Combined sources3
Turni15 – 17Combined sources3
Helixi18 – 20Combined sources3
Beta strandi21 – 23Combined sources3
Beta strandi26 – 31Combined sources6
Helixi34 – 42Combined sources9
Beta strandi51 – 55Combined sources5
Beta strandi58 – 62Combined sources5
Helixi64 – 67Combined sources4
Turni68 – 70Combined sources3
Helixi77 – 81Combined sources5
Beta strandi88 – 91Combined sources4
Beta strandi93 – 96Combined sources4
Beta strandi100 – 103Combined sources4
Helixi115 – 117Combined sources3
Beta strandi123 – 127Combined sources5
Helixi129 – 145Combined sources17
Beta strandi149 – 153Combined sources5
Beta strandi156 – 160Combined sources5
Helixi162 – 171Combined sources10
Beta strandi176 – 178Combined sources3
Helixi183 – 186Combined sources4
Helixi188 – 196Combined sources9
Beta strandi203 – 206Combined sources4
Helixi210 – 214Combined sources5
Beta strandi219 – 221Combined sources3
Helixi233 – 256Combined sources24
Beta strandi260 – 267Combined sources8
Helixi268 – 275Combined sources8
Turni276 – 278Combined sources3
Beta strandi280 – 285Combined sources6
Helixi288 – 290Combined sources3
Helixi291 – 295Combined sources5
Beta strandi297 – 299Combined sources3
Helixi302 – 304Combined sources3
Helixi306 – 308Combined sources3
Helixi311 – 319Combined sources9
Beta strandi324 – 326Combined sources3
Helixi327 – 334Combined sources8
Helixi339 – 351Combined sources13
Beta strandi352 – 355Combined sources4
Turni357 – 359Combined sources3
Helixi364 – 366Combined sources3
Helixi367 – 377Combined sources11
Turni378 – 380Combined sources3
Beta strandi395 – 400Combined sources6
Turni403 – 406Combined sources4
Helixi409 – 434Combined sources26
Beta strandi436 – 448Combined sources13
Beta strandi454 – 460Combined sources7
Helixi466 – 468Combined sources3
Helixi469 – 487Combined sources19
Turni488 – 490Combined sources3
Beta strandi497 – 510Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JIHX-ray2.25A/B1-513[»]
2R8JX-ray3.10A/B1-531[»]
2R8KX-ray3.30A/B1-531[»]
2WTFX-ray2.50A/B1-513[»]
2XGPX-ray2.70A/B1-513[»]
2XGQX-ray2.70A/B1-513[»]
3MFHX-ray2.00A1-513[»]
3MFIX-ray1.76A1-513[»]
3OHAX-ray2.00A1-513[»]
3OHBX-ray2.00A1-513[»]
ProteinModelPortaliQ04049.
SMRiQ04049.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ04049.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 309UmuCPROSITE-ProRule annotationAdd BLAST284

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni625 – 632POL30-binding8

Sequence similaritiesi

Belongs to the DNA polymerase type-Y family.Curated

Phylogenomic databases

HOGENOMiHOG000065930.
InParanoidiQ04049.
KOiK03509.
OMAiFGEESIW.
OrthoDBiEOG092C18P5.

Family and domain databases

Gene3Di3.30.1490.100. 1 hit.
InterProiView protein in InterPro
IPR017061. DNA_pol_eta/kappa/iota/IV.
IPR017961. DNA_pol_Y-fam_little_finger.
IPR001126. UmuC.
PfamiView protein in Pfam
PF00817. IMS. 1 hit.
PF11799. IMS_C. 1 hit.
PIRSFiPIRSF036603. DPol_eta. 1 hit.
SUPFAMiSSF100879. SSF100879. 1 hit.
PROSITEiView protein in PROSITE
PS50173. UMUC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q04049-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKFTWKELI QLGSPSKAYE SSLACIAHID MNAFFAQVEQ MRCGLSKEDP
60 70 80 90 100
VVCVQWNSII AVSYAARKYG ISRMDTIQEA LKKCSNLIPI HTAVFKKGED
110 120 130 140 150
FWQYHDGCGS WVQDPAKQIS VEDHKVSLEP YRRESRKALK IFKSACDLVE
160 170 180 190 200
RASIDEVFLD LGRICFNMLM FDNEYELTGD LKLKDALSNI REAFIGGNYD
210 220 230 240 250
INSHLPLIPE KIKSLKFEGD VFNPEGRDLI TDWDDVILAL GSQVCKGIRD
260 270 280 290 300
SIKDILGYTT SCGLSSTKNV CKLASNYKKP DAQTIVKNDC LLDFLDCGKF
310 320 330 340 350
EITSFWTLGG VLGKELIDVL DLPHENSIKH IRETWPDNAG QLKEFLDAKV
360 370 380 390 400
KQSDYDRSTS NIDPLKTADL AEKLFKLSRG RYGLPLSSRP VVKSMMSNKN
410 420 430 440 450
LRGKSCNSIV DCISWLEVFC AELTSRIQDL EQEYNKIVIP RTVSISLKTK
460 470 480 490 500
SYEVYRKSGP VAYKGINFQS HELLKVGIKF VTDLDIKGKN KSYYPLTKLS
510 520 530 540 550
MTITNFDIID LQKTVVDMFG NQVHTFKSSA GKEDEEKTTS SKADEKTPKL
560 570 580 590 600
ECCKYQVTFT DQKALQEHAD YHLALKLSEG LNGAEESSKN LSFGEKRLLF
610 620 630
SRKRPNSQHT ATPQKKQVTS SKNILSFFTR KK
Length:632
Mass (Da):71,515
Last modified:November 1, 1996 - v1
Checksum:iCFB1A9FBC8AFE39B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33007 Genomic DNA. Translation: AAB64856.1.
BK006938 Genomic DNA. Translation: DAA12259.1.
PIRiS69702.
RefSeqiNP_010707.3. NM_001180727.3.

Genome annotation databases

EnsemblFungiiYDR419W; YDR419W; YDR419W.
GeneIDi852028.
KEGGisce:YDR419W.

Similar proteinsi

Entry informationi

Entry nameiPOLH_YEAST
AccessioniPrimary (citable) accession number: Q04049
Secondary accession number(s): D6VT49
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: November 1, 1996
Last modified: September 27, 2017
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names