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Protein

DNA polymerase eta

Gene

RAD30

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Plays an important role in the repair of UV-induced pyrimidine dimers. Depending on the context, it inserts the correct base, but causes frequent base transitions and transversions. Efficiently incorporates nucleotides opposite to other UV or oxidative DNA damages like O(6)-methylguanine, 7,8-dihydro-8-oxoguanine, 2,6-diamino-4-hydroxy-5-formamidopyrimidine of 2'-deoxyguanosine (FaPydG), or p-benzoquinone DNA adducts.34 Publications

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi30MagnesiumPROSITE-ProRule annotation1
Metal bindingi155MagnesiumPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • chromosome segregation Source: SGD
  • DNA replication Source: UniProtKB-KW
  • error-free translesion synthesis Source: SGD
  • error-prone translesion synthesis Source: SGD
  • mitotic sister chromatid cohesion Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Mutator protein, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair, DNA replication, DNA synthesis

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29960-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase eta (EC:2.7.7.7)
Alternative name(s):
Radiation-sensitive protein 30
Gene namesi
Name:RAD30
Synonyms:DBH1
Ordered Locus Names:YDR419W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR419W.
SGDiS000002827. RAD30.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: SGD
  • nucleus Source: SGD
  • replication fork Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi30D → A: Abolishes DNA polymerase activity. 1 Publication1
Mutagenesisi34F → L: Alters translesion activity. 1 Publication1
Mutagenesisi39E → A: Abolishes DNA polymerase activity. 1 Publication1
Mutagenesisi64Y → F or A: Decreases efficiency of nucleotide incorporation. 1 Publication1
Mutagenesisi67R → A: Decreases efficiency of nucleotide incorporation. 1 Publication1
Mutagenesisi155D → A: Abolishes DNA polymerase activity and increases UV-induced mutations. 1 Publication1
Mutagenesisi156E → A: Decreases efficiency of nucleotide incorporation. 1 Publication1
Mutagenesisi279K → A: Decreases efficiency of nucleotide incorporation. 1 Publication1
Mutagenesisi627F → A: Abolishes POL30-binding; when associated with A-628. 1
Mutagenesisi628F → A: Abolishes POL30-binding; when associated with A-627. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002686981 – 632DNA polymerase etaAdd BLAST632

Proteomic databases

MaxQBiQ04049.
PRIDEiQ04049.

Expressioni

Inductioni

By UV radiation and heat shock. The mRNA is stabilized during stationary phase.2 Publications

Interactioni

Subunit structurei

Interacts with POL30. This interaction is essential for the polymerase eta function.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
POL30P158733EBI-36214,EBI-12993

Protein-protein interaction databases

BioGridi32477. 90 interactors.
DIPiDIP-6500N.
IntActiQ04049. 8 interactors.
MINTiMINT-708283.

Structurei

Secondary structure

1632
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi1 – 5Combined sources5
Helixi6 – 10Combined sources5
Helixi11 – 13Combined sources3
Turni15 – 17Combined sources3
Helixi18 – 20Combined sources3
Beta strandi21 – 23Combined sources3
Beta strandi26 – 31Combined sources6
Helixi34 – 42Combined sources9
Beta strandi51 – 55Combined sources5
Beta strandi58 – 62Combined sources5
Helixi64 – 67Combined sources4
Turni68 – 70Combined sources3
Helixi77 – 81Combined sources5
Beta strandi88 – 91Combined sources4
Beta strandi93 – 96Combined sources4
Beta strandi100 – 103Combined sources4
Helixi115 – 117Combined sources3
Beta strandi123 – 127Combined sources5
Helixi129 – 145Combined sources17
Beta strandi149 – 153Combined sources5
Beta strandi156 – 160Combined sources5
Helixi162 – 171Combined sources10
Beta strandi176 – 178Combined sources3
Helixi183 – 186Combined sources4
Helixi188 – 196Combined sources9
Beta strandi203 – 206Combined sources4
Helixi210 – 214Combined sources5
Beta strandi219 – 221Combined sources3
Helixi233 – 256Combined sources24
Beta strandi260 – 267Combined sources8
Helixi268 – 275Combined sources8
Turni276 – 278Combined sources3
Beta strandi280 – 285Combined sources6
Helixi288 – 290Combined sources3
Helixi291 – 295Combined sources5
Beta strandi297 – 299Combined sources3
Helixi302 – 304Combined sources3
Helixi306 – 308Combined sources3
Helixi311 – 319Combined sources9
Beta strandi324 – 326Combined sources3
Helixi327 – 334Combined sources8
Helixi339 – 351Combined sources13
Beta strandi352 – 355Combined sources4
Turni357 – 359Combined sources3
Helixi364 – 366Combined sources3
Helixi367 – 377Combined sources11
Turni378 – 380Combined sources3
Beta strandi395 – 400Combined sources6
Turni403 – 406Combined sources4
Helixi409 – 434Combined sources26
Beta strandi436 – 448Combined sources13
Beta strandi454 – 460Combined sources7
Helixi466 – 468Combined sources3
Helixi469 – 487Combined sources19
Turni488 – 490Combined sources3
Beta strandi497 – 510Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JIHX-ray2.25A/B1-513[»]
2R8JX-ray3.10A/B1-531[»]
2R8KX-ray3.30A/B1-531[»]
2WTFX-ray2.50A/B1-513[»]
2XGPX-ray2.70A/B1-513[»]
2XGQX-ray2.70A/B1-513[»]
3MFHX-ray2.00A1-513[»]
3MFIX-ray1.76A1-513[»]
3OHAX-ray2.00A1-513[»]
3OHBX-ray2.00A1-513[»]
ProteinModelPortaliQ04049.
SMRiQ04049.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ04049.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 309UmuCPROSITE-ProRule annotationAdd BLAST284

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni625 – 632POL30-binding8

Sequence similaritiesi

Belongs to the DNA polymerase type-Y family.Curated
Contains 1 umuC domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000065930.
InParanoidiQ04049.
KOiK03509.
OMAiGYTTSCG.
OrthoDBiEOG092C18P5.

Family and domain databases

Gene3Di3.30.1490.100. 1 hit.
InterProiIPR017961. DNA_pol_Y-fam_little_finger.
IPR001126. UmuC.
[Graphical view]
PfamiPF00817. IMS. 1 hit.
PF11799. IMS_C. 1 hit.
[Graphical view]
SUPFAMiSSF100879. SSF100879. 1 hit.
PROSITEiPS50173. UMUC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q04049-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKFTWKELI QLGSPSKAYE SSLACIAHID MNAFFAQVEQ MRCGLSKEDP
60 70 80 90 100
VVCVQWNSII AVSYAARKYG ISRMDTIQEA LKKCSNLIPI HTAVFKKGED
110 120 130 140 150
FWQYHDGCGS WVQDPAKQIS VEDHKVSLEP YRRESRKALK IFKSACDLVE
160 170 180 190 200
RASIDEVFLD LGRICFNMLM FDNEYELTGD LKLKDALSNI REAFIGGNYD
210 220 230 240 250
INSHLPLIPE KIKSLKFEGD VFNPEGRDLI TDWDDVILAL GSQVCKGIRD
260 270 280 290 300
SIKDILGYTT SCGLSSTKNV CKLASNYKKP DAQTIVKNDC LLDFLDCGKF
310 320 330 340 350
EITSFWTLGG VLGKELIDVL DLPHENSIKH IRETWPDNAG QLKEFLDAKV
360 370 380 390 400
KQSDYDRSTS NIDPLKTADL AEKLFKLSRG RYGLPLSSRP VVKSMMSNKN
410 420 430 440 450
LRGKSCNSIV DCISWLEVFC AELTSRIQDL EQEYNKIVIP RTVSISLKTK
460 470 480 490 500
SYEVYRKSGP VAYKGINFQS HELLKVGIKF VTDLDIKGKN KSYYPLTKLS
510 520 530 540 550
MTITNFDIID LQKTVVDMFG NQVHTFKSSA GKEDEEKTTS SKADEKTPKL
560 570 580 590 600
ECCKYQVTFT DQKALQEHAD YHLALKLSEG LNGAEESSKN LSFGEKRLLF
610 620 630
SRKRPNSQHT ATPQKKQVTS SKNILSFFTR KK
Length:632
Mass (Da):71,515
Last modified:November 1, 1996 - v1
Checksum:iCFB1A9FBC8AFE39B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33007 Genomic DNA. Translation: AAB64856.1.
BK006938 Genomic DNA. Translation: DAA12259.1.
PIRiS69702.
RefSeqiNP_010707.3. NM_001180727.3.

Genome annotation databases

EnsemblFungiiYDR419W; YDR419W; YDR419W.
GeneIDi852028.
KEGGisce:YDR419W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33007 Genomic DNA. Translation: AAB64856.1.
BK006938 Genomic DNA. Translation: DAA12259.1.
PIRiS69702.
RefSeqiNP_010707.3. NM_001180727.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JIHX-ray2.25A/B1-513[»]
2R8JX-ray3.10A/B1-531[»]
2R8KX-ray3.30A/B1-531[»]
2WTFX-ray2.50A/B1-513[»]
2XGPX-ray2.70A/B1-513[»]
2XGQX-ray2.70A/B1-513[»]
3MFHX-ray2.00A1-513[»]
3MFIX-ray1.76A1-513[»]
3OHAX-ray2.00A1-513[»]
3OHBX-ray2.00A1-513[»]
ProteinModelPortaliQ04049.
SMRiQ04049.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32477. 90 interactors.
DIPiDIP-6500N.
IntActiQ04049. 8 interactors.
MINTiMINT-708283.

Proteomic databases

MaxQBiQ04049.
PRIDEiQ04049.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR419W; YDR419W; YDR419W.
GeneIDi852028.
KEGGisce:YDR419W.

Organism-specific databases

EuPathDBiFungiDB:YDR419W.
SGDiS000002827. RAD30.

Phylogenomic databases

HOGENOMiHOG000065930.
InParanoidiQ04049.
KOiK03509.
OMAiGYTTSCG.
OrthoDBiEOG092C18P5.

Enzyme and pathway databases

BioCyciYEAST:G3O-29960-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ04049.
PROiQ04049.

Family and domain databases

Gene3Di3.30.1490.100. 1 hit.
InterProiIPR017961. DNA_pol_Y-fam_little_finger.
IPR001126. UmuC.
[Graphical view]
PfamiPF00817. IMS. 1 hit.
PF11799. IMS_C. 1 hit.
[Graphical view]
SUPFAMiSSF100879. SSF100879. 1 hit.
PROSITEiPS50173. UMUC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPOLH_YEAST
AccessioniPrimary (citable) accession number: Q04049
Secondary accession number(s): D6VT49
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1860 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.