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Protein

Citrate/oxoglutarate carrier protein

Gene

YHM2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mitochondrial antiporter which catalyzes the transport of citrate and oxoglutarate across the membrane. Also shows specificity for oxaloacetate, and to a lesser extent succinate and fumarate. Transports isocitrate, cis-aconitate and L-malate with very low efficiency. Does not show uniporter activity. Helps to maintain normal citrate levels and NADPH/NADP+ ratios under conditions of oxidative stress. In addition, associates with the mitochondrial nucleoid and binds DNA in vitro, although the relevance of these data in vivo is unclear.2 Publications

Enzyme regulationi

Strongly inhibited by mersalyl, p-chloromercuribenzenesulfonate, mercuric chloride, N-ethylmaleimide, pyridoxal 5'-phosphate, bathophenanthroline, and tannic acid. Partially inhibited by alpha-cyanocinnamate and bromescol purple. Weakly inhibited by butylmalonate and phenylsuccinate. Not inhibited by 1,2,3-benzenetricarboxylate or carboxyatractyloside.1 Publication

Kineticsi

  1. KM=0.16 mM for citrate (with purified protein reconstituted into proteolipososmes)1 Publication
  2. KM=1.2 mM for oxoglutarate (with purified protein reconstituted into proteolipososmes)1 Publication
  1. Vmax=9.8 mmol/min/g enzyme for [14C]citrate/citrate and [14C]citrate/oxoglutarate exchange (with purified protein reconstituted into proteolipososmes)1 Publication
  2. Vmax=10.3 mmol/min/g enzyme for [14C]oxoglutarate/oxoglutarate and [14C]oxoglutarate/citrate exchange (with purified protein reconstituted into proteolipososmes)1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi246 – 259141 PublicationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: SGD
  • tricarboxylate secondary active transmembrane transporter activity Source: SGD

GO - Biological processi

  • alpha-ketoglutarate transport Source: SGD
  • mitochondrial citrate transport Source: SGD
  • mitochondrial genome maintenance Source: SGD
  • tricarboxylic acid transmembrane transport Source: GOC
  • tricarboxylic acid transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transport

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32921-MONOMER.

Protein family/group databases

TCDBi2.A.29.29.1. the mitochondrial carrier (mc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Citrate/oxoglutarate carrier protein1 Publication
Short name:
Coc1p1 Publication
Alternative name(s):
Mitochondrial DNA replication protein YHM2Curated
Gene namesi
Name:YHM2
Ordered Locus Names:YMR241W
ORF Names:YM9408.03
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR241W.
SGDiS000004854. YHM2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei23 – 4422Helical; Name=1Sequence analysisAdd
BLAST
Transmembranei77 – 9721Helical; Name=2Sequence analysisAdd
BLAST
Transmembranei111 – 12717Helical; Name=3Sequence analysisAdd
BLAST
Transmembranei178 – 19821Helical; Name=4Sequence analysisAdd
BLAST
Transmembranei218 – 23821Helical; Name=5Sequence analysisAdd
BLAST
Transmembranei273 – 29422Helical; Name=6Sequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • mitochondrial inner membrane Source: SGD
  • mitochondrial nucleoid Source: SGD
  • mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane, Mitochondrion nucleoid

Pathology & Biotechi

Disruption phenotypei

Shows slow growth and respiration defects in minimal medium. Shows growth defects in acetate-supplemented medium; the phenotype is enhanced by addition of hydrogen peroxide (increased oxidative stress) and/or double knockout of YHM2 and ZWF1. The cytosolic NADPH/NADP+ ratio is decreased compared to wild type; this effect is enhanced in the presence of hydrogen peroxide. The mitochondrial NADPH/NADP+ ratio is increased, but only in the presence of hydrogen peroxide. Levels of cytosolic citrate are reduced, but only in the presence of hydrogen peroxide. Levels of cytosolic oxoglutarate are reduced, but only in the presence of hydrogen peroxide; the effect is enhanced by double knockout of YHM2 and ZWF1. Cells show a very small increase in levels of reactive oxygen species (ROS) following hydrogen peroxide treatment; in double knockouts of YHM2 and ZWF1 there is a large increase in ROS. Overexpression suppresses the temperature-sensitive growth defect of ABF2 in glucose-rich medium.2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi249 – 2535PNRPK → GGGGG: Fails to bind DNA in vitro. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 314314Citrate/oxoglutarate carrier proteinPRO_0000090688Add
BLAST

Proteomic databases

MaxQBiQ04013.
PeptideAtlasiQ04013.
PRIDEiQ04013.

Interactioni

Protein-protein interaction databases

BioGridi35420. 82 interactions.
DIPiDIP-6370N.
IntActiQ04013. 10 interactions.
MINTiMINT-690184.

Structurei

3D structure databases

ProteinModelPortaliQ04013.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati18 – 10083Solcar 1PROSITE-ProRule annotationAdd
BLAST
Repeati107 – 19993Solcar 2PROSITE-ProRule annotationAdd
BLAST
Repeati217 – 30185Solcar 3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 3 Solcar repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000159483.
InParanoidiQ04013.
OMAiVAIRQMT.
OrthoDBiEOG783N5G.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q04013-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSTTNTAAA NVIEKKPVSF SNILLGACLN LSEVTTLGQP LEVVKTTMAA
60 70 80 90 100
NRNFTFLESV KHVWSRGGIL GYYQGLIPWA WIEASTKGAV LLFVSAEAEY
110 120 130 140 150
RFKSLGLNNF ASGILGGVTG GVTQAYLTMG FCTCMKTVEI TRHKSASAGG
160 170 180 190 200
VPQSSWSVFK NIYKKEGIRG INKGVNAVAI RQMTNWGSRF GLSRLVEDGI
210 220 230 240 250
RKITGKTNKD DKLNPFEKIG ASALGGGLSA WNQPIEVIRV EMQSKKEDPN
260 270 280 290 300
RPKNLTVGKT FKYIYQSNGL KGLYRGVTPR IGLGIWQTVF MVGFGDMAKE
310
FVARMTGETP VAKH
Length:314
Mass (Da):34,185
Last modified:November 1, 1997 - v1
Checksum:iEB483C715D195BED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48756 Genomic DNA. Translation: CAA88651.1.
BK006946 Genomic DNA. Translation: DAA10141.1.
PIRiS56055.
RefSeqiNP_013968.1. NM_001182748.1.

Genome annotation databases

EnsemblFungiiYMR241W; YMR241W; YMR241W.
GeneIDi855282.
KEGGisce:YMR241W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48756 Genomic DNA. Translation: CAA88651.1.
BK006946 Genomic DNA. Translation: DAA10141.1.
PIRiS56055.
RefSeqiNP_013968.1. NM_001182748.1.

3D structure databases

ProteinModelPortaliQ04013.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35420. 82 interactions.
DIPiDIP-6370N.
IntActiQ04013. 10 interactions.
MINTiMINT-690184.

Protein family/group databases

TCDBi2.A.29.29.1. the mitochondrial carrier (mc) family.

Proteomic databases

MaxQBiQ04013.
PeptideAtlasiQ04013.
PRIDEiQ04013.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR241W; YMR241W; YMR241W.
GeneIDi855282.
KEGGisce:YMR241W.

Organism-specific databases

EuPathDBiFungiDB:YMR241W.
SGDiS000004854. YHM2.

Phylogenomic databases

HOGENOMiHOG000159483.
InParanoidiQ04013.
OMAiVAIRQMT.
OrthoDBiEOG783N5G.

Enzyme and pathway databases

BioCyciYEAST:G3O-32921-MONOMER.

Miscellaneous databases

NextBioi978917.
PROiQ04013.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "A novel DNA-binding protein bound to the mitochondrial inner membrane restores the null mutation of mitochondrial histone Abf2p in Saccharomyces cerevisiae."
    Cho J.H., Ha S.J., Kao L.R., Megraw T.L., Chae C.-B.
    Mol. Cell. Biol. 18:5712-5723(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, MUTAGENESIS OF 249-PRO--LYS-253.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.
  7. "Identification and functional characterization of a novel mitochondrial carrier for citrate and oxoglutarate in Saccharomyces cerevisiae."
    Castegna A., Scarcia P., Agrimi G., Palmieri L., Rottensteiner H., Spera I., Germinario L., Palmieri F.
    J. Biol. Chem. 285:17359-17370(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiYHM2_YEAST
AccessioniPrimary (citable) accession number: Q04013
Secondary accession number(s): D6W067
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 11, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2930 molecules/cell in log phase SD medium.1 Publication

Caution

Was originally proposed to function in mitochondrial DNA replication and maintenance.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.