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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Leuconostoc mesenteroides subsp. mesenteroides (strain ATCC 8293 / NCDO 523)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei263ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:LEUM_0161
OrganismiLeuconostoc mesenteroides subsp. mesenteroides (strain ATCC 8293 / NCDO 523)
Taxonomic identifieri203120 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLeuconostocaceaeLeuconostoc
Proteomesi
  • UP000000362 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003677021 – 498Glutamate--tRNA ligaseAdd BLAST498

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi203120.LEUM_0161.

Structurei

3D structure databases

ProteinModelPortaliQ03ZR1.
SMRiQ03ZR1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi11 – 21"HIGH" regionUniRule annotationAdd BLAST11
Motifi260 – 264"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20. Bacteria.
COG0008. LUCA.
HOGENOMiHOG000252720.
KOiK09698.
OMAiAYYAFDT.
OrthoDBiPOG091H021W.

Family and domain databases

CDDicd00808. GluRS_core. 1 hit.
Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022. Glu_tRNA_synth_type1. 1 hit.
InterProiView protein in InterPro
IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR033910. GluRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
PfamiView protein in Pfam
PF00749. tRNA-synt_1c. 1 hit.
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiView protein in PROSITE
PS00178. AA_TRNA_LIGASE_I. 1 hit.

Sequencei

Sequence statusi: Complete.

Q03ZR1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEDIRVRYA PSPTGHLHIG NARTAIFNWL FARHYNGTFV IRIEDTDSAR
60 70 80 90 100
NIADGEKSQL ENLAWLGLDW DESPDKPGVY GPYRQSERNE QGIYQEFIDA
110 120 130 140 150
LLASGQAYKS YKTSGQLASE REAQQAAKQA PHYVYEYEGL TNEEREAKYA
160 170 180 190 200
EFEAQGLKPV VRFRVPEEQV YAWDDIVKGH IEIGAKEVGG DWVIQKADGM
210 220 230 240 250
PTYNFAVVVD DHLMKISHVL RGDDHVSNTP KQIMIYEALG WDVPKFGHMA
260 270 280 290 300
LIINGETGKK LSKRDENLLQ FVEQYKELGY QPQAMVNFIG LLGWSPKGED
310 320 330 340 350
EIFSLSEFKE MFDEKRLSKA NAKFDQKKLE WVNNQWMRRD TDAVMPQLIQ
360 370 380 390 400
ELVNAHLISA DDAADKKKWL SEVIKVAGVD GISYTRQIVE LVRKPFFELG
410 420 430 440 450
DITDEMVEYL TSEDGRKVAA AWESTYESLP TDATPADYMS TIRAIQNDLE
460 470 480 490
IKGRNLWNPI RIMTTHEVQG PNLPEMLTLL DKNTVLKTMR DVKEKYLA
Length:498
Mass (Da):56,956
Last modified:November 14, 2006 - v1
Checksum:i91A273292E5E8C26
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000414 Genomic DNA. Translation: ABJ61311.1.
RefSeqiWP_011679119.1. NC_008531.1.

Genome annotation databases

EnsemblBacteriaiABJ61311; ABJ61311; LEUM_0161.
GeneIDi29577456.
KEGGilme:LEUM_0161.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiSYE_LEUMM
AccessioniPrimary (citable) accession number: Q03ZR1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: November 14, 2006
Last modified: June 7, 2017
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families