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Q03ZI1 (DDL_LEUMM) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:LEUM_0264
OrganismLeuconostoc mesenteroides subsp. mesenteroides (strain ATCC 8293 / NCDO 523) [Complete proteome] [HAMAP]
Taxonomic identifier203120 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLeuconostocaceaeLeuconostoc

Protein attributes

Sequence length377 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 377377D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000030463

Regions

Domain140 – 349210ATP-grasp
Nucleotide binding170 – 22556ATP By similarity

Sites

Metal binding3031Magnesium or manganese 1 By similarity
Metal binding3161Magnesium or manganese 1 By similarity
Metal binding3161Magnesium or manganese 2 By similarity
Metal binding3181Magnesium or manganese 2 By similarity

Secondary structure

............................................................... 377
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q03ZI1 [UniParc].

Last modified November 14, 2006. Version 1.
Checksum: D1C6A40A6812AE01

FASTA37741,826
        10         20         30         40         50         60 
MTKKRVALIF GGNSSEHDVS KRSAQNFYNA IEATGKYEII VFAIAQNGFF LDTESSKKIL 

        70         80         90        100        110        120 
ALEDEQPIVD AFMKTVDASD PLARIHALKS AGDFDIFFPV VHGNLGEDGT LQGLFKLLDK 

       130        140        150        160        170        180 
PYVGAPLRGH AVSFDKALTK ELLTVNGIRN TKYIVVDPES ANNWSWDKIV AELGNIVFVK 

       190        200        210        220        230        240 
AANQGSSVGI SRVTNAEEYT EALSDSFQYD YKVLIEEAVN GARELEVGVI GNDQPLVSEI 

       250        260        270        280        290        300 
GAHTVPNQGS GDGWYDYNNK FVDNSAVHFE IPAQLSPEVT KEVKQMALDA YKVLNLRGEA 

       310        320        330        340        350        360 
RMDFLLDENN VPYLGEPNTL PGFTNMSLFK RLWDYSDINN AKLVDMLIDY GFEDFAQNKK 

       370 
LSYSFVSLGE EKIGKFN 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000414 Genomic DNA. Translation: ABJ61391.1.
RefSeqYP_817764.1. NC_008531.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1EHIX-ray2.38A/B1-377[»]
ProteinModelPortalQ03ZI1.
SMRQ03ZI1. Positions 3-362.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING203120.LEUM_0264.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABJ61391; ABJ61391; LEUM_0264.
GeneID4422165.
KEGGlme:LEUM_0264.
PATRIC22408513. VBILeuMes50664_0271.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMAQIDVIFP.
OrthoDBEOG64BQ73.

Enzyme and pathway databases

BioCycLMES203120:GJ8T-264-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ03ZI1.

Entry information

Entry nameDDL_LEUMM
AccessionPrimary (citable) accession number: Q03ZI1
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 14, 2006
Last modified: July 9, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways