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Protein

Hydroxyethylthiazole kinase 1

Gene

thiM1

Organism
Leuconostoc mesenteroides subsp. mesenteroides (strain ATCC 8293 / NCDO 523)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei38 – 381Substrate; via amide nitrogenUniRule annotation
Binding sitei113 – 1131ATPUniRule annotation
Binding sitei158 – 1581ATPUniRule annotation
Binding sitei185 – 1851Substrate; via amide nitrogenUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. hydroxyethylthiazole kinase activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. thiamine biosynthetic process Source: UniProtKB-KW
  2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLMES203120:GJ8T-485-MONOMER.
UniPathwayiUPA00060; UER00139.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinase 1UniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase 1UniRule annotation
Short name:
TH kinase 1UniRule annotation
Short name:
Thz kinase 1UniRule annotation
Gene namesi
Name:thiM1UniRule annotation
Ordered Locus Names:LEUM_0485
OrganismiLeuconostoc mesenteroides subsp. mesenteroides (strain ATCC 8293 / NCDO 523)
Taxonomic identifieri203120 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLeuconostocaceaeLeuconostoc
ProteomesiUP000000362 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 259259Hydroxyethylthiazole kinase 1PRO_0000383881Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi203120.LEUM_0485.

Structurei

3D structure databases

ProteinModelPortaliQ03YX1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2145.
HOGENOMiHOG000114352.
KOiK00878.
OMAiMAVTENI.
OrthoDBiEOG628F8M.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.

Sequencei

Sequence statusi: Complete.

Q03YX1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNITDIRTKS PLVLTYANFV TPQFVANVVN VVGASPLMSR ELAEFKELAG
60 70 80 90 100
IANAVIINTG TLQKVEINDI IKLSQEAYQL GKPVVLDPVA VSVPFRSKAI
110 120 130 140 150
TQFLASGHVD VIRGNAAEIA WFADIDFASQ GIDATGKGDV IEIAQRAAKK
160 170 180 190 200
TGAVIALSGA CDVVSDGQYT QTLDINVEQL SSIVGTGDAL SSLIGAFIAD
210 220 230 240 250
GLKVPNVMNA MATFKLAGQK AATKTNQPGS FTNQLLDELF VIDNIDVQNF

VKESVLNHG
Length:259
Mass (Da):27,406
Last modified:November 14, 2006 - v1
Checksum:i967ECB5AFE317221
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000414 Genomic DNA. Translation: ABJ61601.1.
RefSeqiWP_011679325.1. NC_008531.1.
YP_817974.1. NC_008531.1.

Genome annotation databases

EnsemblBacteriaiABJ61601; ABJ61601; LEUM_0485.
KEGGilme:LEUM_0485.
PATRICi22408957. VBILeuMes50664_0485.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000414 Genomic DNA. Translation: ABJ61601.1.
RefSeqiWP_011679325.1. NC_008531.1.
YP_817974.1. NC_008531.1.

3D structure databases

ProteinModelPortaliQ03YX1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi203120.LEUM_0485.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABJ61601; ABJ61601; LEUM_0485.
KEGGilme:LEUM_0485.
PATRICi22408957. VBILeuMes50664_0485.

Phylogenomic databases

eggNOGiCOG2145.
HOGENOMiHOG000114352.
KOiK00878.
OMAiMAVTENI.
OrthoDBiEOG628F8M.

Enzyme and pathway databases

UniPathwayiUPA00060; UER00139.
BioCyciLMES203120:GJ8T-485-MONOMER.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 8293 / NCDO 523.

Entry informationi

Entry nameiTHIM1_LEUMM
AccessioniPrimary (citable) accession number: Q03YX1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: November 14, 2006
Last modified: April 29, 2015
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.