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Q03YH4 (SYL_LEUMM) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:LEUM_0635
OrganismLeuconostoc mesenteroides subsp. mesenteroides (strain ATCC 8293 / NCDO 523) [Complete proteome] [HAMAP]
Taxonomic identifier203120 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLeuconostocaceaeLeuconostoc

Protein attributes

Sequence length808 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 808808Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009366

Regions

Motif40 – 5112"HIGH" region HAMAP-Rule MF_00049
Motif580 – 5845"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5831ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q03YH4 [UniParc].

Last modified November 14, 2006. Version 1.
Checksum: 437DB9D2DDD86F42

FASTA80892,689
        10         20         30         40         50         60 
MAYDHKSIEA KWQKYWDEHE TFRAPDKSEK EKYYVMDMFP YPSGQGLHVG HPEGYTATDI 

        70         80         90        100        110        120 
MSRYKRAKGF NVLHPMGWDA FGLPAEQYAI KTGNNPAGFT NKNVQVFKNQ IKSLGFSIDW 

       130        140        150        160        170        180 
SREINTTDPE YYKWSQWIFE KLYEKGLAYE DEIMVNWAPD FPGGGIVVAN EEVIDGKTER 

       190        200        210        220        230        240 
GGYPVYRKPM KQWVLRITAY ADRLLEDLED LDWPEAIKEQ QRHWIGKSIG ASVFFNIADS 

       250        260        270        280        290        300 
DKQIEVYTTR PDTLFGAQYM VLSPEHELVD EITTLEQKEA IANYKKEVET KSDLERTDLN 

       310        320        330        340        350        360 
KDKTGAFTGA YATNPINGEK LPIWIADYVL SSYGTGAIMA VPAHDDRDWE FAKKFNLPIK 

       370        380        390        400        410        420 
AVIEGGNVEE AAYTDDGTHI NSGFLDGLNK QEAIDKAISW LEEHNAGHQQ INYRLRDWIF 

       430        440        450        460        470        480 
SRQRYWGEPI PVIHWEDGTQ TLVPESELPL RLPEMTQEQL KPSGTGESPL ANATDWLNVT 

       490        500        510        520        530        540 
RKDGVKGRRE TNTMPQWAGS SWYFLRYVDP HNSEALADPE KLKYWMNVDL YVGGAEHAVL 

       550        560        570        580        590        600 
HLLYARFWHK FLYDLGVVPT KEPFQKLVNQ GMILGENHEK MSKSKGNVVN PDEIVNNYGA 

       610        620        630        640        650        660 
DTLRVYEMFM GPLTQSKPWS EDGVSGSRRW LDRVWRLLVD EEDKLRDHVT TVNKGDLDKI 

       670        680        690        700        710        720 
YHQTVKKVSE DLENMRFNTA ISQLMVFVNE AYKSEALPVQ YMDGFVQMLA PIAPHLAEEL 

       730        740        750        760        770        780 
WSKLGHEEDI SYVSWPTYDE SQLVEDSVEI IFQVNGKVRG KATVARNIDK DAMLDYAKAD 

       790        800 
ENVQNFISGK EIRKIIAIPG KFVNIVVG 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000414 Genomic DNA. Translation: ABJ61748.1.
RefSeqYP_818121.1. NC_008531.1.

3D structure databases

ProteinModelPortalQ03YH4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING203120.LEUM_0635.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABJ61748; ABJ61748; LEUM_0635.
GeneID4422468.
KEGGlme:LEUM_0635.
PATRIC22409255. VBILeuMes50664_0631.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycLMES203120:GJ8T-635-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_LEUMM
AccessionPrimary (citable) accession number: Q03YH4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 14, 2006
Last modified: July 9, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries