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Protein

Formate--tetrahydrofolate ligase

Gene

fhs

Organism
Leuconostoc mesenteroides subsp. mesenteroides (strain ATCC 8293 / NCDO 523)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.UniRule annotation

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi64 – 718ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. formate-tetrahydrofolate ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. folic acid-containing compound biosynthetic process Source: InterPro
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLMES203120:GJ8T-701-MONOMER.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate--tetrahydrofolate ligaseUniRule annotation (EC:6.3.4.3UniRule annotation)
Alternative name(s):
Formyltetrahydrofolate synthetaseUniRule annotation
Short name:
FHSUniRule annotation
Short name:
FTHFSUniRule annotation
Gene namesi
Name:fhsUniRule annotation
Ordered Locus Names:LEUM_0701
OrganismiLeuconostoc mesenteroides subsp. mesenteroides (strain ATCC 8293 / NCDO 523)
Taxonomic identifieri203120 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLeuconostocaceaeLeuconostoc
ProteomesiUP000000362 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 554554Formate--tetrahydrofolate ligasePRO_0000300527Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi203120.LEUM_0701.

Structurei

3D structure databases

ProteinModelPortaliQ03YB1.
SMRiQ03YB1. Positions 4-552.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the formate--tetrahydrofolate ligase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
KOiK01938.
OMAiCMVKTHL.
OrthoDBiEOG6PCPSP.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03YB1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQTDIEIAQS VEIKPITKIA TTAGLQANEI EPYGYDKAKI NLDPSIVRKQ
60 70 80 90 100
ELGKLILVTS INPTPAGEGK STVTVGLADA LNLADKKTMI ALREPSLGPV
110 120 130 140 150
MGLKGGATGG GFAQVVPMAD INLHFTGDFH ALTSAHDTLA ALLDNSIHQG
160 170 180 190 200
NPLNIDPRRI LWKRVVDIND RELRHVTVGL GGPTSGVPRE DGFDITVASE
210 220 230 240 250
LMAILTLSTD LMDMKERIKR IVVGYTYSKE PVTVADLGVA GALTVLLKDA
260 270 280 290 300
IKPNLVQTLA HTPAIIHGGP FANIAQGTNS ILATKTALKL ADYVVTEAGF
310 320 330 340 350
GADLGGEKFL DVKVPLLGKT PDTIVIVATV RALKHHGGVA LSNLNNEDVT
360 370 380 390 400
ALRNGLENLG QHLTAMNRYG VPVLVAINRF TSDTEAELQV IKDYVQQFGA
410 420 430 440 450
TAYTTEVWAK GGAGAQELAA AVIKKSAQEA DFTPLYQADD SAIDKLNTIV
460 470 480 490 500
QTIYGGTGVE LSTKAQKQLQ DFKKYGWDKL PIIMAKTQYS FTDDAKKLGA
510 520 530 540 550
PKDFKIYIRE FVPKLGAGFL VALTGSIMTM PGLPKHPAAL DIDIDENGTI

TGLF
Length:554
Mass (Da):59,178
Last modified:November 14, 2006 - v1
Checksum:i40AF51A0C05941EC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000414 Genomic DNA. Translation: ABJ61811.1.
RefSeqiYP_818184.1. NC_008531.1.

Genome annotation databases

EnsemblBacteriaiABJ61811; ABJ61811; LEUM_0701.
KEGGilme:LEUM_0701.
PATRICi22409391. VBILeuMes50664_0698.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000414 Genomic DNA. Translation: ABJ61811.1.
RefSeqiYP_818184.1. NC_008531.1.

3D structure databases

ProteinModelPortaliQ03YB1.
SMRiQ03YB1. Positions 4-552.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi203120.LEUM_0701.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABJ61811; ABJ61811; LEUM_0701.
KEGGilme:LEUM_0701.
PATRICi22409391. VBILeuMes50664_0698.

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
KOiK01938.
OMAiCMVKTHL.
OrthoDBiEOG6PCPSP.

Enzyme and pathway databases

UniPathwayiUPA00193.
BioCyciLMES203120:GJ8T-701-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 8293 / NCDO 523.

Entry informationi

Entry nameiFTHS_LEUMM
AccessioniPrimary (citable) accession number: Q03YB1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: November 14, 2006
Last modified: April 1, 2015
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.