Q03Y28 (KHSE_LEUMM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homoserine kinase
Short name=HK
Short name=HSK
EC=2.7.1.39

Gene names

Name:	thrB
Ordered Locus Names:	LEUM_0785

Organism

Leuconostoc mesenteroides subsp. mesenteroides (strain ATCC 8293 / NCDO 523) [Complete proteome] [HAMAP]

Taxonomic identifier

203120 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Leuconostoc

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate By similarity. HAMAP MF_00384
Catalytic activity	ATP + L-homoserine = ADP + O-phospho-L-homoserine. HAMAP MF_00384
Pathway	Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5. HAMAP MF_00384
Subcellular location	Cytoplasm Potential HAMAP MF_00384.
Sequence similarities	Belongs to the GHMP kinase family. Homoserine kinase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Threonine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW homoserine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 292	292	Homoserine kinase HAMAP MF_00384		PRO_1000049140
Regions
	Nucleotide binding	80 – 90	11	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q03Y28 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: 6F2EB5896A09D704	FASTA	292	31,287
	10 20 30 40 50 60 MIKITVPATS ANIGPGFDSL GVALKLYLTL EVYEETSEWQ VIHDYGANMP SDVNNFIVKT 70 80 90 100 110 120 ALLLAPNLTP HRIVVKSDIP LARGLGSSSS ALLAGLAMAN VLADMRLNND ALLKQATALE 130 140 150 160 170 180 GHPDNVAPAL LGGSVAAFYD GEQVYHAPLS LPKDINFITF IPNYELLTTE ARNALPAKMT 190 200 210 220 230 240 FKDSVAASAI SNTLTAALNA GDFNTARVLI EKDQFHEQAR AHLAPHLKII RDTAHQLEII 250 260 270 280 290 GTYLSGAGPT VITLVSKDNA TKLLKVLTNM ALPGKLLLLE PDYTGLQITK NN

References

[1]

"Comparative genomics of the lactic acid bacteria." Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V., Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V., Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M., Hawkins T. , Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A., Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W., Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y., Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R., O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T., Richardson P., Kozyavkin S., Weimer B.C., Mills D.A. Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006) [PubMed: 17030793] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8293 / NCDO 523.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000414 Genomic DNA. Translation: ABJ61894.1.
RefSeq	YP_818267.1. NC_008531.1.
3D structure databases
ProteinModelPortal	Q03Y28.
ModBase	Search...
Protein-protein interaction databases
STRING	Q03Y28.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4422618.
GenomeReviews	Gene locus LEUM_0785 in contig CP000414_GR.
KEGG	lme:LEUM_0785.
NMPDR	fig|203120.1.peg.364.
PATRIC	22409557. VBILeuMes50664_0780.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0083.
HOGENOM	HBG646290.
OMA	NNALMST.
PhylomeDB	Q03Y28.
ProtClustDB	PRK01212.
Enzyme and pathway databases
BioCyc	LMES203120:LEUM_0785-MONOMER.
Family and domain databases
HAMAP	MF_00384. Homoser_kinase. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR006203. GHMP_knse_ATP-bd_CS. IPR000870. Homoserine_kinase. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00872.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF000676. Homoser_kin. 1 hit.
PRINTS	PR00958. HOMSERKINASE.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00191. ThrB. 1 hit.
PROSITE	PS00627. GHMP_KINASES_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KHSE_LEUMM

Accession

Primary (citable) accession number: Q03Y28

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 14, 2006
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program