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Q03WU9 (TRMB_LEUMM) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:LEUM_1225
OrganismLeuconostoc mesenteroides subsp. mesenteroides (strain ATCC 8293 / NCDO 523) [Complete proteome] [HAMAP]
Taxonomic identifier203120 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLeuconostoc

Protein attributes

Sequence length219 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 219219tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000288171

Regions

Region199 – 2024Substrate binding By similarity

Sites

Active site1221 By similarity
Binding site461S-adenosyl-L-methionine By similarity
Binding site711S-adenosyl-L-methionine By similarity
Binding site1001S-adenosyl-L-methionine By similarity
Binding site1221S-adenosyl-L-methionine By similarity
Binding site1261Substrate By similarity
Binding site1581Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q03WU9 [UniParc].

Last modified November 14, 2006. Version 1.
Checksum: CF4E71AD5280019D

FASTA21925,462
        10         20         30         40         50         60 
MHLRSKPWAS DWLAEHSDIV IDQDRATAQI GQWQSLFDQE QPIHLEIGSG KGQFILGMAL 

        70         80         90        100        110        120 
AHPEINYIGM EIQETAIAIA ARKSFDQVGT LPNLRYIYGN GNGVETYFEK GEVSKVYLNF 

       130        140        150        160        170        180 
SDPWPKKRHE SRRLTYKSFL KSYEAVLPEH GEVEFKTDNR HLFEYSMVSF MDYGMRWTPE 

       190        200        210 
DYTLDLHADE DKVQGNVETE YEQKFMAKGQ PIYKIKAHF 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000414 Genomic DNA. Translation: ABJ62323.1.
RefSeqYP_818696.1. NC_008531.1.

3D structure databases

ProteinModelPortalQ03WU9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING203120.LEUM_1225.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABJ62323; ABJ62323; LEUM_1225.
GeneID4422990.
KEGGlme:LEUM_1225.
PATRIC22410439. VBILeuMes50664_1222.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000251689.
KOK03439.
OMAKTDDDEL.
OrthoDBEOG6K6VBC.
ProtClustDBPRK00121.

Enzyme and pathway databases

BioCycLMES203120:GJ8T-1225-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_LEUMM
AccessionPrimary (citable) accession number: Q03WU9
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: November 14, 2006
Last modified: April 16, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways