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Q03WL0

- BGAL_LEUMM

UniProt

Q03WL0 - BGAL_LEUMM

Protein

Beta-galactosidase

Gene

lacZ

Organism
Leuconostoc mesenteroides subsp. mesenteroides (strain ATCC 8293 / NCDO 523)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 1 (14 Nov 2006)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

    Cofactori

    Binds 2 magnesium ions per monomer.UniRule annotation
    Binds 1 sodium ion per monomer.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei97 – 971SubstrateUniRule annotation
    Metal bindingi197 – 1971SodiumUniRule annotation
    Binding sitei197 – 1971SubstrateUniRule annotation
    Sitei352 – 3521Transition state stabilizerUniRule annotation
    Sitei386 – 3861Transition state stabilizerUniRule annotation
    Metal bindingi411 – 4111Magnesium 1UniRule annotation
    Metal bindingi413 – 4131Magnesium 1UniRule annotation
    Active sitei456 – 4561Proton donorUniRule annotation
    Metal bindingi456 – 4561Magnesium 1UniRule annotation
    Binding sitei456 – 4561SubstrateUniRule annotation
    Active sitei532 – 5321NucleophileUniRule annotation
    Metal bindingi592 – 5921Magnesium 2UniRule annotation
    Metal bindingi596 – 5961Sodium; via carbonyl oxygenUniRule annotation
    Metal bindingi599 – 5991SodiumUniRule annotation
    Binding sitei599 – 5991SubstrateUniRule annotation
    Binding sitei1006 – 10061SubstrateUniRule annotation

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC
    2. carbohydrate binding Source: InterPro
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding, Sodium

    Enzyme and pathway databases

    BioCyciLMES203120:GJ8T-1316-MONOMER.

    Protein family/group databases

    CAZyiGH2. Glycoside Hydrolase Family 2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
    Short name:
    Beta-galUniRule annotation
    Alternative name(s):
    LactaseUniRule annotation
    Gene namesi
    Name:lacZUniRule annotation
    Ordered Locus Names:LEUM_1316
    OrganismiLeuconostoc mesenteroides subsp. mesenteroides (strain ATCC 8293 / NCDO 523)
    Taxonomic identifieri203120 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLeuconostocaceaeLeuconostoc
    ProteomesiUP000000362: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. beta-galactosidase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10361036Beta-galactosidasePRO_0000367003Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi203120.LEUM_1316.

    Structurei

    3D structure databases

    ProteinModelPortaliQ03WL0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni532 – 5354Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 2 family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG3250.
    HOGENOMiHOG000252443.
    KOiK01190.
    OMAiNPPFVPK.
    OrthoDBiEOG6XWV0T.

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPiMF_01687. Beta_gal.
    InterProiIPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view]
    PRINTSiPR00132. GLHYDRLASE2.
    SMARTiSM01038. Bgal_small_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q03WL0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTSIKQILAR HDWENPVVTN WNRLPLHTSM SYANERNKRE IKQPRKSLNG     50
    PWQFSYFENL SDIDEEWRKK DLPTSKIIHV PSNWQLQGDY DVPVYTNVTY 100
    PFPVNPPYVP TENPVGAYSK KVFLDNKWLA DNTESHVVFN GVGSAFYLWV 150
    NGEWVGYSED SRLPAEFDIT EELRAGENRI AVLVLKWSKG SYFEDQDMWR 200
    MSGIFRDVDL IRVPKTRFQD LAIETKLDED LDDATVEVRA QLVGNSADNL 250
    SVTAELFYHG MSLFKATEQF GNRVIDERGT NDGQVSLELP VKNPALWSAE 300
    VPNLYDIKVS LHDGEENYQI ENKKVGIRKV QIKDGLLTLN NQPLLIRGVN 350
    KHEFNSKTGY YVDEKTMIDD IRMMKEHNFN AVRLSHYPNA SRWYELCDQY 400
    GLYLVDEANI ETHGVKPMNY LTNDPKYLPL MMERVTRMVQ RDYNHPSIII 450
    WSLGNESGYG HNHDAMYQWI KNTDPSRPIQ YEGGGADTPA TDIIAPMYAR 500
    VDQDQVEEVN SKWAIKKWIG LSKENRPLIL CEYAHSMGNS LGGFNKYWEA 550
    FEKYPRLQGG FIWDWVDQGL LTKNNEGQSY YAYGGDFGDY PNDRQFSLDG 600
    LLFPDRTPKP ALLEAKYCQQ YFAFQLEKDP TGKVNYMTVS NKHLFKTVND 650
    ATLIYQILSN DQVIETKKIK LNLAPQTEER VSLNFSDNSN EDVYMNCQIV 700
    QDSTDGLIRS GTLLAYKQFI LRNKPIMISD VRSSDDYEDF LINDATDSLS 750
    ISLDDAIWQF NKRTGWLSNW IKNGQEKVLT PLKDQFSRAA LDNDIGVSEV 800
    TNIDPNAWFE RWQATGFNHL NEKLVQFNWT ALKDEVRITT QHQFLSPIDQ 850
    HIMFISSKEY RINHVGDLKV YVDVWRQVAD PQPARIGLSV QINATTDAVT 900
    YSGLGPMENY PDRRSAAIRG KWDASLKELY TPYVFPSENG LRTEVAYLKF 950
    DHHVIRALEQ RFSFNLSQFS QAQLSAVTHQ HLLKPEEGVW LNIDGYHMGV 1000
    GGDDSWSPSV SPEFLLSNDH YHYSFSWSNA EGEANV 1036
    Length:1,036
    Mass (Da):119,401
    Last modified:November 14, 2006 - v1
    Checksum:iCD70E17BFE291D64
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000414 Genomic DNA. Translation: ABJ62412.1.
    RefSeqiWP_011680026.1. NC_008531.1.
    YP_818785.1. NC_008531.1.

    Genome annotation databases

    EnsemblBacteriaiABJ62412; ABJ62412; LEUM_1316.
    GeneIDi4423064.
    KEGGilme:LEUM_1316.
    PATRICi22410617. VBILeuMes50664_1311.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000414 Genomic DNA. Translation: ABJ62412.1 .
    RefSeqi WP_011680026.1. NC_008531.1.
    YP_818785.1. NC_008531.1.

    3D structure databases

    ProteinModelPortali Q03WL0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 203120.LEUM_1316.

    Protein family/group databases

    CAZyi GH2. Glycoside Hydrolase Family 2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABJ62412 ; ABJ62412 ; LEUM_1316 .
    GeneIDi 4423064.
    KEGGi lme:LEUM_1316.
    PATRICi 22410617. VBILeuMes50664_1311.

    Phylogenomic databases

    eggNOGi COG3250.
    HOGENOMi HOG000252443.
    KOi K01190.
    OMAi NPPFVPK.
    OrthoDBi EOG6XWV0T.

    Enzyme and pathway databases

    BioCyci LMES203120:GJ8T-1316-MONOMER.

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPi MF_01687. Beta_gal.
    InterProi IPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00132. GLHYDRLASE2.
    SMARTi SM01038. Bgal_small_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 8293 / NCDO 523.

    Entry informationi

    Entry nameiBGAL_LEUMM
    AccessioniPrimary (citable) accession number: Q03WL0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: November 14, 2006
    Last modified: October 1, 2014
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3