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Q03WL0 (BGAL_LEUMM) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase
Short name=Beta-gal
EC=3.2.1.23
Alternative name(s):
Lactase
Gene names
Name:lacZ
Ordered Locus Names:LEUM_1316
OrganismLeuconostoc mesenteroides subsp. mesenteroides (strain ATCC 8293 / NCDO 523) [Complete proteome] [HAMAP]
Taxonomic identifier203120 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Lactobacillales › Leuconostoc

Protein attributes

Sequence length1036 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. HAMAP MF_01687

Cofactor

Binds 2 magnesium ions per monomer By similarity. HAMAP MF_01687

Binds 1 sodium ion per monomer By similarity. HAMAP MF_01687

Subunit structure

Homotetramer By similarity. HAMAP MF_01687

Sequence similarities

Belongs to the glycosyl hydrolase 2 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10361036Beta-galactosidase HAMAP MF_01687
PRO_0000367003

Regions

Region532 – 5354Substrate binding By similarity

Sites

Active site4561Proton donor By similarity
Active site5321Nucleophile By similarity
Metal binding1971Sodium By similarity
Metal binding4111Magnesium 1 By similarity
Metal binding4131Magnesium 1 By similarity
Metal binding4561Magnesium 1 By similarity
Metal binding5921Magnesium 2 By similarity
Metal binding5961Sodium; via carbonyl oxygen By similarity
Metal binding5991Sodium By similarity
Binding site971Substrate By similarity
Binding site1971Substrate By similarity
Binding site4561Substrate By similarity
Binding site5991Substrate By similarity
Binding site10061Substrate By similarity
Site3521Transition state stabilizer By similarity
Site3861Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
Q03WL0 [UniParc].

Last modified November 14, 2006. Version 1.
Checksum: CD70E17BFE291D64

FASTA1,036119,401
        10         20         30         40         50         60 
MTSIKQILAR HDWENPVVTN WNRLPLHTSM SYANERNKRE IKQPRKSLNG PWQFSYFENL 

        70         80         90        100        110        120 
SDIDEEWRKK DLPTSKIIHV PSNWQLQGDY DVPVYTNVTY PFPVNPPYVP TENPVGAYSK 

       130        140        150        160        170        180 
KVFLDNKWLA DNTESHVVFN GVGSAFYLWV NGEWVGYSED SRLPAEFDIT EELRAGENRI 

       190        200        210        220        230        240 
AVLVLKWSKG SYFEDQDMWR MSGIFRDVDL IRVPKTRFQD LAIETKLDED LDDATVEVRA 

       250        260        270        280        290        300 
QLVGNSADNL SVTAELFYHG MSLFKATEQF GNRVIDERGT NDGQVSLELP VKNPALWSAE 

       310        320        330        340        350        360 
VPNLYDIKVS LHDGEENYQI ENKKVGIRKV QIKDGLLTLN NQPLLIRGVN KHEFNSKTGY 

       370        380        390        400        410        420 
YVDEKTMIDD IRMMKEHNFN AVRLSHYPNA SRWYELCDQY GLYLVDEANI ETHGVKPMNY 

       430        440        450        460        470        480 
LTNDPKYLPL MMERVTRMVQ RDYNHPSIII WSLGNESGYG HNHDAMYQWI KNTDPSRPIQ 

       490        500        510        520        530        540 
YEGGGADTPA TDIIAPMYAR VDQDQVEEVN SKWAIKKWIG LSKENRPLIL CEYAHSMGNS 

       550        560        570        580        590        600 
LGGFNKYWEA FEKYPRLQGG FIWDWVDQGL LTKNNEGQSY YAYGGDFGDY PNDRQFSLDG 

       610        620        630        640        650        660 
LLFPDRTPKP ALLEAKYCQQ YFAFQLEKDP TGKVNYMTVS NKHLFKTVND ATLIYQILSN 

       670        680        690        700        710        720 
DQVIETKKIK LNLAPQTEER VSLNFSDNSN EDVYMNCQIV QDSTDGLIRS GTLLAYKQFI 

       730        740        750        760        770        780 
LRNKPIMISD VRSSDDYEDF LINDATDSLS ISLDDAIWQF NKRTGWLSNW IKNGQEKVLT 

       790        800        810        820        830        840 
PLKDQFSRAA LDNDIGVSEV TNIDPNAWFE RWQATGFNHL NEKLVQFNWT ALKDEVRITT 

       850        860        870        880        890        900 
QHQFLSPIDQ HIMFISSKEY RINHVGDLKV YVDVWRQVAD PQPARIGLSV QINATTDAVT 

       910        920        930        940        950        960 
YSGLGPMENY PDRRSAAIRG KWDASLKELY TPYVFPSENG LRTEVAYLKF DHHVIRALEQ 

       970        980        990       1000       1010       1020 
RFSFNLSQFS QAQLSAVTHQ HLLKPEEGVW LNIDGYHMGV GGDDSWSPSV SPEFLLSNDH 

      1030 
YHYSFSWSNA EGEANV

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000414 Genomic DNA. Translation: ABJ62412.1.
RefSeqYP_818785.1. NC_008531.1.

3D structure databases

ProteinModelPortalQ03WL0.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ03WL0.

Protein family/group databases

CAZyGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4423064.
GenomeReviewsGene locus LEUM_1316 in contig CP000414_GR.
KEGGlme:LEUM_1316.
PATRIC22410617. VBILeuMes50664_1311.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG3250.
HOGENOMHBG670643.
OMARDWENPA.
PhylomeDBQ03WL0.
ProtClustDBPRK09525.

Enzyme and pathway databases

BioCycLMES203120:LEUM_1316-MONOMER.

Family and domain databases

HAMAPMF_01687. Beta_gal.
[Tree]
InterProIPR004199. B-gal_small/dom_5.
IPR008979. Galactose-bd-like.
IPR011013. Glyco_hydro-type_carb-bd.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_subgr_catalytic.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
Gene3DG3DSA:2.60.40.320. Glyco_hydro_2/20_Ig-like. 2 hits.
G3DSA:2.70.98.10. Glyco_hydro_42_D5. 1 hit.
G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
KOK01190.
PfamPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSPR00132. GLHYDRLASE2.
SMARTSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMSSF49785. Gal_bind_like. 1 hit.
SSF74650. Gal_mut_like. 1 hit.
SSF49303. Glyco_hydro_2Ig. 2 hits.
SSF51445. Glyco_hydro_cat. 1 hit.
PROSITEPS00719. GLYCOSYL_HYDROL_F2_1. False negative.
PS00608. GLYCOSYL_HYDROL_F2_2. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL_LEUMM
AccessionPrimary (citable) accession number: Q03WL0
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: November 14, 2006
Last modified: January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

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