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Q03WL0

- BGAL_LEUMM

UniProt

Q03WL0 - BGAL_LEUMM

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Protein

Beta-galactosidase

Gene

lacZ

Organism
Leuconostoc mesenteroides subsp. mesenteroides (strain ATCC 8293 / NCDO 523)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Binds 2 magnesium ions per monomer.UniRule annotation
Binds 1 sodium ion per monomer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei97 – 971SubstrateUniRule annotation
Metal bindingi197 – 1971SodiumUniRule annotation
Binding sitei197 – 1971SubstrateUniRule annotation
Sitei352 – 3521Transition state stabilizerUniRule annotation
Sitei386 – 3861Transition state stabilizerUniRule annotation
Metal bindingi411 – 4111Magnesium 1UniRule annotation
Metal bindingi413 – 4131Magnesium 1UniRule annotation
Active sitei456 – 4561Proton donorUniRule annotation
Metal bindingi456 – 4561Magnesium 1UniRule annotation
Binding sitei456 – 4561SubstrateUniRule annotation
Active sitei532 – 5321NucleophileUniRule annotation
Metal bindingi592 – 5921Magnesium 2UniRule annotation
Metal bindingi596 – 5961Sodium; via carbonyl oxygenUniRule annotation
Metal bindingi599 – 5991SodiumUniRule annotation
Binding sitei599 – 5991SubstrateUniRule annotation
Binding sitei1006 – 10061SubstrateUniRule annotation

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Enzyme and pathway databases

BioCyciLMES203120:GJ8T-1316-MONOMER.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
Short name:
Beta-galUniRule annotation
Alternative name(s):
LactaseUniRule annotation
Gene namesi
Name:lacZUniRule annotation
Ordered Locus Names:LEUM_1316
OrganismiLeuconostoc mesenteroides subsp. mesenteroides (strain ATCC 8293 / NCDO 523)
Taxonomic identifieri203120 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLeuconostocaceaeLeuconostoc
ProteomesiUP000000362: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10361036Beta-galactosidasePRO_0000367003Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi203120.LEUM_1316.

Structurei

3D structure databases

ProteinModelPortaliQ03WL0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni532 – 5354Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG3250.
HOGENOMiHOG000252443.
KOiK01190.
OMAiNPPFVPK.
OrthoDBiEOG6XWV0T.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.

Sequencei

Sequence statusi: Complete.

Q03WL0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTSIKQILAR HDWENPVVTN WNRLPLHTSM SYANERNKRE IKQPRKSLNG
60 70 80 90 100
PWQFSYFENL SDIDEEWRKK DLPTSKIIHV PSNWQLQGDY DVPVYTNVTY
110 120 130 140 150
PFPVNPPYVP TENPVGAYSK KVFLDNKWLA DNTESHVVFN GVGSAFYLWV
160 170 180 190 200
NGEWVGYSED SRLPAEFDIT EELRAGENRI AVLVLKWSKG SYFEDQDMWR
210 220 230 240 250
MSGIFRDVDL IRVPKTRFQD LAIETKLDED LDDATVEVRA QLVGNSADNL
260 270 280 290 300
SVTAELFYHG MSLFKATEQF GNRVIDERGT NDGQVSLELP VKNPALWSAE
310 320 330 340 350
VPNLYDIKVS LHDGEENYQI ENKKVGIRKV QIKDGLLTLN NQPLLIRGVN
360 370 380 390 400
KHEFNSKTGY YVDEKTMIDD IRMMKEHNFN AVRLSHYPNA SRWYELCDQY
410 420 430 440 450
GLYLVDEANI ETHGVKPMNY LTNDPKYLPL MMERVTRMVQ RDYNHPSIII
460 470 480 490 500
WSLGNESGYG HNHDAMYQWI KNTDPSRPIQ YEGGGADTPA TDIIAPMYAR
510 520 530 540 550
VDQDQVEEVN SKWAIKKWIG LSKENRPLIL CEYAHSMGNS LGGFNKYWEA
560 570 580 590 600
FEKYPRLQGG FIWDWVDQGL LTKNNEGQSY YAYGGDFGDY PNDRQFSLDG
610 620 630 640 650
LLFPDRTPKP ALLEAKYCQQ YFAFQLEKDP TGKVNYMTVS NKHLFKTVND
660 670 680 690 700
ATLIYQILSN DQVIETKKIK LNLAPQTEER VSLNFSDNSN EDVYMNCQIV
710 720 730 740 750
QDSTDGLIRS GTLLAYKQFI LRNKPIMISD VRSSDDYEDF LINDATDSLS
760 770 780 790 800
ISLDDAIWQF NKRTGWLSNW IKNGQEKVLT PLKDQFSRAA LDNDIGVSEV
810 820 830 840 850
TNIDPNAWFE RWQATGFNHL NEKLVQFNWT ALKDEVRITT QHQFLSPIDQ
860 870 880 890 900
HIMFISSKEY RINHVGDLKV YVDVWRQVAD PQPARIGLSV QINATTDAVT
910 920 930 940 950
YSGLGPMENY PDRRSAAIRG KWDASLKELY TPYVFPSENG LRTEVAYLKF
960 970 980 990 1000
DHHVIRALEQ RFSFNLSQFS QAQLSAVTHQ HLLKPEEGVW LNIDGYHMGV
1010 1020 1030
GGDDSWSPSV SPEFLLSNDH YHYSFSWSNA EGEANV
Length:1,036
Mass (Da):119,401
Last modified:November 14, 2006 - v1
Checksum:iCD70E17BFE291D64
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000414 Genomic DNA. Translation: ABJ62412.1.
RefSeqiYP_818785.1. NC_008531.1.

Genome annotation databases

EnsemblBacteriaiABJ62412; ABJ62412; LEUM_1316.
GeneIDi4423064.
KEGGilme:LEUM_1316.
PATRICi22410617. VBILeuMes50664_1311.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000414 Genomic DNA. Translation: ABJ62412.1 .
RefSeqi YP_818785.1. NC_008531.1.

3D structure databases

ProteinModelPortali Q03WL0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 203120.LEUM_1316.

Protein family/group databases

CAZyi GH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABJ62412 ; ABJ62412 ; LEUM_1316 .
GeneIDi 4423064.
KEGGi lme:LEUM_1316.
PATRICi 22410617. VBILeuMes50664_1311.

Phylogenomic databases

eggNOGi COG3250.
HOGENOMi HOG000252443.
KOi K01190.
OMAi NPPFVPK.
OrthoDBi EOG6XWV0T.

Enzyme and pathway databases

BioCyci LMES203120:GJ8T-1316-MONOMER.

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_01687. Beta_gal.
InterProi IPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
PRINTSi PR00132. GLHYDRLASE2.
SMARTi SM01038. Bgal_small_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 8293 / NCDO 523.

Entry informationi

Entry nameiBGAL_LEUMM
AccessioniPrimary (citable) accession number: Q03WL0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: November 14, 2006
Last modified: October 29, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3