SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q03WL0

- BGAL_LEUMM

UniProt

Q03WL0 - BGAL_LEUMM

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Beta-galactosidase
Gene
lacZ, LEUM_1316
Organism
Leuconostoc mesenteroides subsp. mesenteroides (strain ATCC 8293 / NCDO 523)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Binds 2 magnesium ions per monomer By similarity.UniRule annotation
Binds 1 sodium ion per monomer By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei97 – 971Substrate By similarity
Metal bindingi197 – 1971Sodium By similarity
Binding sitei197 – 1971Substrate By similarity
Sitei352 – 3521Transition state stabilizer By similarity
Sitei386 – 3861Transition state stabilizer By similarity
Metal bindingi411 – 4111Magnesium 1 By similarity
Metal bindingi413 – 4131Magnesium 1 By similarity
Active sitei456 – 4561Proton donor By similarity
Metal bindingi456 – 4561Magnesium 1 By similarity
Binding sitei456 – 4561Substrate By similarity
Active sitei532 – 5321Nucleophile By similarity
Metal bindingi592 – 5921Magnesium 2 By similarity
Metal bindingi596 – 5961Sodium; via carbonyl oxygen By similarity
Metal bindingi599 – 5991Sodium By similarity
Binding sitei599 – 5991Substrate By similarity
Binding sitei1006 – 10061Substrate By similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Enzyme and pathway databases

BioCyciLMES203120:GJ8T-1316-MONOMER.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Short name:
Beta-gal
Alternative name(s):
Lactase
Gene namesi
Name:lacZ
Ordered Locus Names:LEUM_1316
OrganismiLeuconostoc mesenteroides subsp. mesenteroides (strain ATCC 8293 / NCDO 523)
Taxonomic identifieri203120 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLeuconostocaceaeLeuconostoc
ProteomesiUP000000362: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10361036Beta-galactosidaseUniRule annotation
PRO_0000367003Add
BLAST

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi203120.LEUM_1316.

Structurei

3D structure databases

ProteinModelPortaliQ03WL0.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni532 – 5354Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3250.
HOGENOMiHOG000252443.
KOiK01190.
OMAiNPPFVPK.
OrthoDBiEOG6XWV0T.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.

Sequencei

Sequence statusi: Complete.

Q03WL0-1 [UniParc]FASTAAdd to Basket

« Hide

MTSIKQILAR HDWENPVVTN WNRLPLHTSM SYANERNKRE IKQPRKSLNG     50
PWQFSYFENL SDIDEEWRKK DLPTSKIIHV PSNWQLQGDY DVPVYTNVTY 100
PFPVNPPYVP TENPVGAYSK KVFLDNKWLA DNTESHVVFN GVGSAFYLWV 150
NGEWVGYSED SRLPAEFDIT EELRAGENRI AVLVLKWSKG SYFEDQDMWR 200
MSGIFRDVDL IRVPKTRFQD LAIETKLDED LDDATVEVRA QLVGNSADNL 250
SVTAELFYHG MSLFKATEQF GNRVIDERGT NDGQVSLELP VKNPALWSAE 300
VPNLYDIKVS LHDGEENYQI ENKKVGIRKV QIKDGLLTLN NQPLLIRGVN 350
KHEFNSKTGY YVDEKTMIDD IRMMKEHNFN AVRLSHYPNA SRWYELCDQY 400
GLYLVDEANI ETHGVKPMNY LTNDPKYLPL MMERVTRMVQ RDYNHPSIII 450
WSLGNESGYG HNHDAMYQWI KNTDPSRPIQ YEGGGADTPA TDIIAPMYAR 500
VDQDQVEEVN SKWAIKKWIG LSKENRPLIL CEYAHSMGNS LGGFNKYWEA 550
FEKYPRLQGG FIWDWVDQGL LTKNNEGQSY YAYGGDFGDY PNDRQFSLDG 600
LLFPDRTPKP ALLEAKYCQQ YFAFQLEKDP TGKVNYMTVS NKHLFKTVND 650
ATLIYQILSN DQVIETKKIK LNLAPQTEER VSLNFSDNSN EDVYMNCQIV 700
QDSTDGLIRS GTLLAYKQFI LRNKPIMISD VRSSDDYEDF LINDATDSLS 750
ISLDDAIWQF NKRTGWLSNW IKNGQEKVLT PLKDQFSRAA LDNDIGVSEV 800
TNIDPNAWFE RWQATGFNHL NEKLVQFNWT ALKDEVRITT QHQFLSPIDQ 850
HIMFISSKEY RINHVGDLKV YVDVWRQVAD PQPARIGLSV QINATTDAVT 900
YSGLGPMENY PDRRSAAIRG KWDASLKELY TPYVFPSENG LRTEVAYLKF 950
DHHVIRALEQ RFSFNLSQFS QAQLSAVTHQ HLLKPEEGVW LNIDGYHMGV 1000
GGDDSWSPSV SPEFLLSNDH YHYSFSWSNA EGEANV 1036
Length:1,036
Mass (Da):119,401
Last modified:November 14, 2006 - v1
Checksum:iCD70E17BFE291D64
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000414 Genomic DNA. Translation: ABJ62412.1.
RefSeqiWP_011680026.1. NC_008531.1.
YP_818785.1. NC_008531.1.

Genome annotation databases

EnsemblBacteriaiABJ62412; ABJ62412; LEUM_1316.
GeneIDi4423064.
KEGGilme:LEUM_1316.
PATRICi22410617. VBILeuMes50664_1311.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000414 Genomic DNA. Translation: ABJ62412.1 .
RefSeqi WP_011680026.1. NC_008531.1.
YP_818785.1. NC_008531.1.

3D structure databases

ProteinModelPortali Q03WL0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 203120.LEUM_1316.

Protein family/group databases

CAZyi GH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABJ62412 ; ABJ62412 ; LEUM_1316 .
GeneIDi 4423064.
KEGGi lme:LEUM_1316.
PATRICi 22410617. VBILeuMes50664_1311.

Phylogenomic databases

eggNOGi COG3250.
HOGENOMi HOG000252443.
KOi K01190.
OMAi NPPFVPK.
OrthoDBi EOG6XWV0T.

Enzyme and pathway databases

BioCyci LMES203120:GJ8T-1316-MONOMER.

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_01687. Beta_gal.
InterProi IPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
PRINTSi PR00132. GLHYDRLASE2.
SMARTi SM01038. Bgal_small_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 8293 / NCDO 523.

Entry informationi

Entry nameiBGAL_LEUMM
AccessioniPrimary (citable) accession number: Q03WL0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: November 14, 2006
Last modified: September 3, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi