Q03WI4 (Q03WI4_LEUMM) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Peptide deformylase HAMAP MF_00163
Short name=PDF HAMAP MF_00163
EC=3.5.1.88 HAMAP MF_00163

Alternative name(s):
Polypeptide deformylase HAMAP MF_00163

Gene names

Name:	def HAMAP MF_00163
Ordered Locus Names:	LEUM_1345

Organism

Leuconostoc mesenteroides subsp. mesenteroides (strain ATCC 8293 / NCDO 523) [Complete proteome] [HAMAP]

Taxonomic identifier

203120 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Leuconostoc

Protein attributes

Sequence length	192 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions By similarity. HAMAP MF_00163
Catalytic activity	Formyl-L-methionyl peptide + H2O = formate + methionyl peptide. HAMAP MF_00163 SAAS SAAS000181
Cofactor	Binds 1 Fe2+ ion By similarity. HAMAP MF_00163
Sequence similarities	Belongs to the polypeptide deformylase family. HAMAP MF_00163 RuleBase RU003335

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP MF_00163 SAAS SAAS000181
Ligand	Iron HAMAP MF_00163 Metal-binding HAMAP MF_00163 SAAS SAAS000181
Molecular function	Hydrolase HAMAP MF_00163 SAAS SAAS000181 EMBL ABJ62438.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Molecular function	iron ion binding Inferred from electronic annotation. Source: InterPro peptide deformylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	163	1	By similarity HAMAP MF_00163
	Metal binding	119	1	Iron By similarity HAMAP MF_00163
	Metal binding	162	1	Iron By similarity HAMAP MF_00163
	Metal binding	166	1	Iron By similarity HAMAP MF_00163

Sequences

	Sequence		Length	Mass (Da)
	Q03WI4 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: 9CDB51408FDB458C	FASTA	192	21,845
	10 20 30 40 50 60 MTIRFTMDKI TRDGDPVLRK TAASVPFPLS EEHAQLAKDM MEYLVVSQDE KQNEKYGLRP 70 80 90 100 110 120 GVGLAAPQVG YSLRMSSILI PALDPEDTID EPYFKGTIFN PVIISESVKR GALNVGEGCL 130 140 150 160 170 180 SVDEDVPGFV PRAYRITVRY QDEDGNKQTI KLRDYPAIVF QHEIDHLHGH LYYDHINMQS 190 PWEVDEDTKY ID

References

[1]

"Comparative genomics of the lactic acid bacteria." Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V., Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V., Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M., Hawkins T. , Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A., Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W., Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y., Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R., O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T., Richardson P., Kozyavkin S., Weimer B.C., Mills D.A. Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006) [PubMed: 17030793] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000414 Genomic DNA. Translation: ABJ62438.1.
RefSeq	YP_818811.1. NC_008531.1.
3D structure databases
ProteinModelPortal	Q03WI4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q03WI4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4423093.
GenomeReviews	Gene locus LEUM_1345 in contig CP000414_GR.
KEGG	lme:LEUM_1345.
PATRIC	22410673. VBILeuMes50664_1336.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0242.
HOGENOM	HBG665227.
OMA	LKNGEGC.
ProtClustDB	PRK00150.
Family and domain databases
HAMAP	MF_00163. Pep_deformylase. [Tree]
InterPro	IPR000181. Fmet_deformylase. IPR023635. Peptide_deformylase. [Graphical view]
Gene3D	G3DSA:3.90.45.10. Fmet_deformylase. 1 hit.
KO	K01462.
PANTHER	PTHR10458. Fmet_deformylase. 1 hit.
Pfam	PF01327. Pep_deformylase. 1 hit. [Graphical view]
PIRSF	PIRSF004749. Pep_def. 1 hit.
PRINTS	PR01576. PDEFORMYLASE.
SUPFAM	SSF56420. Fmet_deformylase. 1 hit.
TIGRFAMs	TIGR00079. Pept_deformyl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

Q03WI4_LEUMM

Accession

Primary (citable) accession number: Q03WI4

Entry history

Integrated into UniProtKB/TrEMBL:	November 14, 2006
Last sequence update:	November 14, 2006
Last modified:	December 14, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)