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Q03W70 (ASSY_LEUMM) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Argininosuccinate synthase

EC=6.3.4.5
Alternative name(s):
Citrulline--aspartate ligase
Gene names
Name:argG
Ordered Locus Names:LEUM_1459
OrganismLeuconostoc mesenteroides subsp. mesenteroides (strain ATCC 8293 / NCDO 523) [Complete proteome] [HAMAP]
Taxonomic identifier203120 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLeuconostoc

Protein attributes

Sequence length408 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP-Rule MF_00005

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP-Rule MF_00005

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00005

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00005.

Sequence similarities

Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Arginine biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processarginine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

argininosuccinate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 408408Argininosuccinate synthase HAMAP-Rule MF_00005
PRO_0000321312

Regions

Nucleotide binding8 – 169ATP By similarity

Sites

Binding site861Citrulline By similarity
Binding site1161ATP; via amide nitrogen By similarity
Binding site1181Aspartate By similarity
Binding site1221Aspartate By similarity
Binding site1221Citrulline By similarity
Binding site1231Aspartate By similarity
Binding site1261Citrulline By similarity
Binding site1741Citrulline By similarity
Binding site2591Citrulline By similarity
Binding site2711Citrulline By similarity

Sequences

Sequence LengthMass (Da)Tools
Q03W70 [UniParc].

Last modified November 14, 2006. Version 1.
Checksum: 50269E18745D5E45

FASTA40844,912
        10         20         30         40         50         60 
MTDTLVLAYS GGLDTSVAIP WLKDKGYDVI AVVLDVGQHG KNLNEIQAKA LKVGAKQSIV 

        70         80         90        100        110        120 
IDAKAEFADK YVAPVIKANM MYEGEYPMVS ALSRPLIIKK LVDIAHENDA VAIAHGSTGH 

       130        140        150        160        170        180 
GNDQVRFEAA IHALDPDMKI EAPIRDFQWS REEEIQYAQE HDVPVPIDLD SPYSIDENLW 

       190        200        210        220        230        240 
GRANEAGILE NPWNQAPEDA FALTTAIEDA PDTPEFIDVT FEAGVPVALN GEVLSLEKLI 

       250        260        270        280        290        300 
VAVNEIAGKH GIGRIDHIEN RLVGIKSREI YEAPAAAVLM TAHKDLEDLT LERDVAHFKP 

       310        320        330        340        350        360 
IVEQQLANLV YEAKWVSPLF DSLMAFIDST QQNVNGVVKM KLFKGNATAV ARQSEHNSLY 

       370        380        390        400 
DEDLATYTSS SSFDQASAVG FIKLWTLSNT VYEQVNHVHS QAKKNTVK 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000414 Genomic DNA. Translation: ABJ62552.1.
RefSeqYP_818925.1. NC_008531.1.

3D structure databases

ProteinModelPortalQ03W70.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING203120.LEUM_1459.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABJ62552; ABJ62552; LEUM_1459.
GeneID4423189.
KEGGlme:LEUM_1459.
PATRIC22410901. VBILeuMes50664_1450.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0137.
HOGENOMHOG000230093.
KOK01940.
OMAIYNGYWW.
OrthoDBEOG6K9QCV.
ProtClustDBPRK00509.

Enzyme and pathway databases

BioCycLMES203120:GJ8T-1459-MONOMER.
UniPathwayUPA00068; UER00113.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPMF_00005. Arg_succ_synth_type1.
InterProIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsTIGR00032. argG. 1 hit.
PROSITEPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameASSY_LEUMM
AccessionPrimary (citable) accession number: Q03W70
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: November 14, 2006
Last modified: February 19, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways