Reviewed, UniProtKB/Swiss-Prot Q03TT0 (GUAC_LACBA)

Names and origin

Protein names

Recommended name:
    GMP reductase
    EC=1.7.1.7
Alternative name(s):
    Guanosine 5'-monophosphate oxidoreductase
      Short name=Guanosine monophosphate reductase

Gene names

Name:	guaC
Ordered Locus Names:	LVIS_0226

Organism

Lactobacillus brevis (strain ATCC 367 / JCM 1170) [Complete proteome] [HAMAP]

Taxonomic identifier

387344 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides By similarity.
Catalytic activity	Inosine 5'-phosphate + NH3 + NADP+ = guanosine 5'-phosphate + NADPH. HAMAP MF_01511
Sequence similarities	Belongs to the IMPDH/GMPR family. GuaC type 2 subfamily.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW purine nucleotide metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	GMP reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	GMP reductase HAMAP MF_01511		PRO_0000292050
Regions
	Nucleotide binding	206 – 229	24	NADP Potential
Sites
	Active site	177	1	Thioimidate intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q03TT0-1 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: DD7A0C66BF41F14B	FASTA	328	36,006
	10 20 30 40 50 60 MHPAEVFDYE DIQLVPNKCI IDSRSEADTS IEFGPRRFKI PVVPANMETV INEPLAVWLA 70 80 90 100 110 120 EHDYFYVMHR FQPEDRRGFI ERMQAKSLFA SISVGVKPEE VTFIDELATA GLTPEYITID 130 140 150 160 170 180 IAHGHSDAVI RMIHHIKQQL PNSFVIAGNV GTPEAVRELE NAGADATKVG IGPGKACITK 190 200 210 220 230 240 LKTGFGTGGW QLAAVRLCAK AARKPIVADG GIRYNGDIAK SIRFGASMVM IGSMLAGHEQ 250 260 270 280 290 300 SPGSLLTIDG RTFKQYWGSA SEKQKGAYRN VEGKQMLVPY RGDIDQTLTA MEEDLQSAIS 310 320 YAGGRNLLDI RTVDYVIVKS SILNGDNY

References

[1]

"Comparative genomics of the lactic acid bacteria." Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V., Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V., Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M., Hawkins T. , Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A., Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W., Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y., Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R., O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T., Richardson P., Kozyavkin S., Weimer B.C., Mills D.A. Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006) [PubMed: 17030793] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000416 Genomic DNA. Translation: ABJ63392.1.
RefSeq	YP_794423.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q03TT0.
Genome annotation databases
GeneID	4413048.
GenomeReviews	Gene locus LVIS_0226 in contig CP000416_GR.
KEGG	lbr:LVIS_0226.
NMPDR	fig|387344.8.peg.218.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q03TT0.
OMA	NSRSECD.
Family and domain databases
HAMAP	MF_01511. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005994. GMP_reduct2. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DH_GMPRt. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00478. IMPDH. 1 hit. [Graphical view]
PIRSF	PIRSF036500. GMP_red_Firmic. 1 hit.
TIGRFAMs	TIGR01306. GMP_reduct_2. 1 hit.
PROSITE	PS00487. IMP_DH_GMP_RED. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAC_LACBA

Accession

Primary (citable) accession number: Q03TT0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	November 14, 2006
Last modified:	November 3, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)