Q03TS9 (PURA_LACBA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenylosuccinate synthetase
Short name=AMPSase
Short name=AdSS
EC=6.3.4.4

Alternative name(s):
IMP--aspartate ligase

Gene names

Name:	purA
Ordered Locus Names:	LVIS_0227

Organism

Lactobacillus brevis (strain ATCC 367 / JCM 1170) [Complete proteome] [HAMAP]

Taxonomic identifier

387344 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	428 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP By similarity. HAMAP MF_00011
Catalytic activity	GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP. HAMAP MF_00011
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00011
Pathway	Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. HAMAP MF_00011
Subunit structure	Homodimer By similarity. HAMAP MF_00011
Subcellular location	Cytoplasm By similarity HAMAP MF_00011.
Sequence similarities	Belongs to the adenylosuccinate synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylosuccinate synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 428	428	Adenylosuccinate synthetase HAMAP MF_00011		PRO_1000000841
Regions
	Nucleotide binding	12 – 18	7	GTP By similarity
	Nucleotide binding	40 – 42	3	GTP By similarity
	Nucleotide binding	329 – 331	3	GTP By similarity
	Nucleotide binding	411 – 413	3	GTP By similarity
	Region	13 – 16	4	IMP binding By similarity
	Region	38 – 41	4	IMP binding By similarity
	Region	297 – 303	7	Substrate binding By similarity
Sites
	Active site	13	1	Proton acceptor By similarity
	Active site	41	1	Proton donor By similarity
	Metal binding	13	1	Magnesium By similarity
	Metal binding	40	1	Magnesium; via carbonyl oxygen By similarity
	Binding site	127	1	IMP By similarity
	Binding site	141	1	IMP; shared with dimeric partner By similarity
	Binding site	222	1	IMP By similarity
	Binding site	237	1	IMP By similarity
	Binding site	301	1	IMP By similarity
	Binding site	303	1	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q03TS9 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: 11CC669F31DF5E25	FASTA	428	46,870
	10 20 30 40 50 60 MSSTVVVGSQ WGDEGKGKIT DFLSEKADVI ARYQGGDNAG HTIVFNGQTF KLRLIPSGIF 70 80 90 100 110 120 YADKTSVIGN GVVLNPQSLI EELTYLHDNG VATDNLRISD RAHVILPYHI LLDQAQEKAK 130 140 150 160 170 180 ASKIGTTNKG IGPAYMDKAE RIGIRVADLL DHDIFAEKLH QNLIEKNKVL TKLYDEEPLN 190 200 210 220 230 240 YDDIFETYYA LGQQLKGYVT DTSVVINDAL DAGKNVLFEG AQGVMLDIDH GTYPFVTSSN 250 260 270 280 290 300 PVAGGVTIGS GVGPTKIDHV VGVCKAYTSR VGDGPFPTEL FDEIGDTIRE TGHEYGTVTK 310 320 330 340 350 360 RPRRIGWFDS VVLRHAKRVS GLTTLSLNCL DVLTGLKTVK ICKAYQLNGE TIYHYPASLV 370 380 390 400 410 420 DLDACQPVYE ELPGWDEDIT HCRSVAELPA NAQTYVKRLA ELVGVEIATL SVGPDREQTN ILQDVWNA

References

[1]

"Comparative genomics of the lactic acid bacteria." Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V., Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V., Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M., Hawkins T. , Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A., Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W., Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y., Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R., O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T., Richardson P., Kozyavkin S., Weimer B.C., Mills D.A. Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006) [PubMed: 17030793] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 367 / JCM 1170.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000416 Genomic DNA. Translation: ABJ63393.1.
RefSeq	YP_794424.1. NC_008497.1.
3D structure databases
ProteinModelPortal	Q03TS9.
ModBase	Search...
Protein-protein interaction databases
STRING	Q03TS9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4413049.
GenomeReviews	Gene locus LVIS_0227 in contig CP000416_GR.
KEGG	lbr:LVIS_0227.
NMPDR	fig|387344.8.peg.219.
PATRIC	22199422. VBILacBre134470_0223.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0104.
HOGENOM	HBG658237.
OMA	YVLGIIK.
PhylomeDB	Q03TS9.
Enzyme and pathway databases
BioCyc	LBRE387344:LVIS_0227-MONOMER.
Family and domain databases
HAMAP	MF_00011. Adenylosucc_synth. [Tree]
InterPro	IPR018220. Adenylosuccinate_synthase_AS. IPR001114. Adenylosuccinate_synthetase. [Graphical view]
KO	K01939.
PANTHER	PTHR11846. Asucc_synthtase. 1 hit.
Pfam	PF00709. Adenylsucc_synt. 1 hit. [Graphical view]
SMART	SM00788. Adenylsucc_synt. 1 hit. [Graphical view]
TIGRFAMs	TIGR00184. PurA. 1 hit.
PROSITE	PS01266. ADENYLOSUCCIN_SYN_1. 1 hit. PS00513. ADENYLOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURA_LACBA

Accession

Primary (citable) accession number: Q03TS9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 14, 2006
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program