Q03T06 (ALR_LACBA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine racemase
EC=5.1.1.1

Gene names

Name:	alr
Ordered Locus Names:	LVIS_0508

Organism

Lactobacillus brevis (strain ATCC 367 / JCM 1170) [Complete proteome] [HAMAP]

Taxonomic identifier

387344 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the D-alanine required for cell wall biosynthesis By similarity. HAMAP MF_01201
Catalytic activity	L-alanine = D-alanine. HAMAP MF_01201
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_01201
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP MF_01201 Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_01201
Sequence similarities	Belongs to the alanine racemase family.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanine metabolic process Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alanine racemase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 373	373	Alanine racemase HAMAP MF_01201		PRO_1000065996
Sites
	Active site	40	1	Proton acceptor; specific for D-alanine By similarity
	Active site	268	1	Proton acceptor; specific for L-alanine By similarity
Amino acid modifications
	Modified residue	40	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q03T06 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: 1180DC9B38EDC91C	FASTA	373	40,412
	10 20 30 40 50 60 MVVGVHRPTQ LVIDRQALHD NIQAEVTRLA SGCELFMVVK ANGYGHGAVQ VAQVAKEAGA 70 80 90 100 110 120 TGFCVAILDE ALELRAAGFN EPILVLGVTE PEDAPLMAQQ HISATVAATD WLVAADAYLA 130 140 150 160 170 180 LAQPTAPLQV HLGLDTGMGR IGFKTTTELT TAVSYLATHA TLNFEGIFTH FATADSPDET 190 200 210 220 230 240 YFKQQVAKWQ TLTAALKERP RYVHVSNSAT SLWHAACNDN MVRFGVAGYG LNPSGTAIPA 250 260 270 280 290 300 PYALKPALSL TSQLVHSKQV VAGESVGYGA TYTAQQTEWV GTVPIGYADG YERRLQGFHV 310 320 330 340 350 360 LVDGQACEII GRVCMDQLMI RLPHDYARGT RVTLIGRDGD HVITLQEMAD YCQTIHYELA 370 CGFTSRLPRV YRH

References

[1]

"Comparative genomics of the lactic acid bacteria." Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V., Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V., Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M., Hawkins T. , Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A., Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W., Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y., Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R., O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T., Richardson P., Kozyavkin S., Weimer B.C., Mills D.A. Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006) [PubMed: 17030793] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 367 / JCM 1170.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000416 Genomic DNA. Translation: ABJ63666.1.
RefSeq	YP_794697.1. NC_008497.1.
3D structure databases
ProteinModelPortal	Q03T06.
SMR	Q03T06. Positions 5-371.
ModBase	Search...
Protein-protein interaction databases
STRING	Q03T06.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4414376.
GenomeReviews	Gene locus LVIS_0508 in contig CP000416_GR.
KEGG	lbr:LVIS_0508.
NMPDR	fig|387344.8.peg.489.
PATRIC	22199980. VBILacBre134470_0498.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0787.
HOGENOM	HBG712172.
OMA	IRISSNA.
PhylomeDB	Q03T06.
ProtClustDB	CLSK821195.
Enzyme and pathway databases
BioCyc	LBRE387344:LVIS_0508-MONOMER.
Family and domain databases
HAMAP	MF_01201. Ala_racemase. [Tree]
InterPro	IPR000821. Ala_racemase. IPR009006. Ala_racemase/Decarboxylase_C. IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR020622. Ala_racemase_pyridoxalP-BS. [Graphical view]
Gene3D	G3DSA:2.40.37.10. Ala_racemase/Decarboxylase_C. 1 hit.
KO	K01775.
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
SMART	SM01005. Ala_racemase_C. 1 hit. [Graphical view]
SUPFAM	SSF50621. Racem_decarbox_C. 1 hit.
TIGRFAMs	TIGR00492. Alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR_LACBA

Accession

Primary (citable) accession number: Q03T06

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 14, 2006
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program