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Protein

Glutamate--tRNA ligase 2

Gene

gltX2

Organism
Lactobacillus brevis (strain ATCC 367 / JCM 1170)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei262 – 2621ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. tRNA binding Source: InterPro

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLBRE387344:GJ8S-579-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligase 2UniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetase 2UniRule annotation
Short name:
GluRS 2UniRule annotation
Gene namesi
Name:gltX2UniRule annotation
Ordered Locus Names:LVIS_0579
OrganismiLactobacillus brevis (strain ATCC 367 / JCM 1170)
Taxonomic identifieri387344 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000001652: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 496496Glutamate--tRNA ligase 2PRO_0000367696Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi387344.LVIS_0579.

Structurei

3D structure databases

ProteinModelPortaliQ03SU7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi12 – 2211"HIGH" regionAdd
BLAST
Motifi259 – 2635"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
KOiK09698.
OMAiATMGITH.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03SU7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAKNSIRVRY APSPTGHLHI GNARTAIFNY LFARHNKGKF IIRIEDTDTK
60 70 80 90 100
RNIEDGERSQ LENLKWLGLD WDEGPDVGGD YGPYRQSERR DIYTPLIQQL
110 120 130 140 150
LDEGKAYESY RTEEELQADR EAQKARKEMP HYEYEYAGMS EEEKQAAIDE
160 170 180 190 200
AKAKGLKPVI RFRVPKDEVF AWDDMVKGHV SFNSDTIGGD FVIAKRDGMP
210 220 230 240 250
TYNFAVVVDD HKMAISHVFR GDDHVANTPK QLMIYQAFGW EAPKFGHMSL
260 270 280 290 300
IISKDTGKKL SKRDESVLQF IEQYRSLGYL PEAMFNFILL LGWSPVGEEE
310 320 330 340 350
IFNKKQFIKM YDETRLSSSP ATFDSDKLEW LNNRYVKDAD DSTIMDLALK
360 370 380 390 400
QLIKAGNMPA NPDAKTIEWA RQLINLYKRQ MSYMAQINDM ASVFFEEPEQ
410 420 430 440 450
VSGEAFDEIN NDTAPVVLKE FAAQIEALDL FDSVEIFKVI KSVQAKTGIK
460 470 480 490
GRQLWMPIRI AVTHEMHGPE LPESIELVGR ETALEHIHQT LAQLKG
Length:496
Mass (Da):56,862
Last modified:November 14, 2006 - v1
Checksum:iC035E63FF99146BC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000416 Genomic DNA. Translation: ABJ63725.1.
RefSeqiYP_794756.1. NC_008497.1.

Genome annotation databases

EnsemblBacteriaiABJ63725; ABJ63725; LVIS_0579.
GeneIDi4413286.
KEGGilbr:LVIS_0579.
PATRICi22200122. VBILacBre134470_0559.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000416 Genomic DNA. Translation: ABJ63725.1.
RefSeqiYP_794756.1. NC_008497.1.

3D structure databases

ProteinModelPortaliQ03SU7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi387344.LVIS_0579.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABJ63725; ABJ63725; LVIS_0579.
GeneIDi4413286.
KEGGilbr:LVIS_0579.
PATRICi22200122. VBILacBre134470_0559.

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
KOiK09698.
OMAiATMGITH.
OrthoDBiEOG6DRPF7.

Enzyme and pathway databases

BioCyciLBRE387344:GJ8S-579-MONOMER.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 367 / JCM 1170.

Entry informationi

Entry nameiSYE2_LACBA
AccessioniPrimary (citable) accession number: Q03SU7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: November 14, 2006
Last modified: January 7, 2015
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.