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Protein

Fumarate hydratase class II

Gene

fumC

Organism
Lactobacillus brevis (strain ATCC 367 / JCM 1170)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.UniRule annotation

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.UniRule annotation

Catalytic activityi

(S)-malate = fumarate + H2O.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Fumarate hydratase class II (fumC)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei182SubstrateUniRule annotation1
Active sitei183Proton donor/acceptorUniRule annotation1
Active sitei313UniRule annotation1
Binding sitei314SubstrateUniRule annotation1
Sitei326Important for catalytic activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotationSAAS annotation
Biological processTricarboxylic acid cycleUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00223; UER01007.

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase class IIUniRule annotation (EC:4.2.1.2UniRule annotation)
Short name:
Fumarase CUniRule annotation
Alternative name(s):
Aerobic fumaraseUniRule annotation
Iron-independent fumaraseUniRule annotation
Gene namesi
Name:fumCUniRule annotation
Ordered Locus Names:LVIS_0714Imported
OrganismiLactobacillus brevis (strain ATCC 367 / JCM 1170)Imported
Taxonomic identifieri387344 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
Proteomesi
  • UP000001652 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi387344.LVIS_0714.

Structurei

3D structure databases

ProteinModelPortaliQ03SG5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 337Lyase_1InterPro annotationAdd BLAST327
Domaini404 – 456FumaraseC_CInterPro annotationAdd BLAST53

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni99 – 101Substrate bindingUniRule annotation3
Regioni135 – 137Substrate bindingUniRule annotation3
Regioni319 – 321Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C9Q. Bacteria.
COG0114. LUCA.
HOGENOMiHOG000061736.
KOiK01679.
OMAiVSFTDNC.

Family and domain databases

CDDicd01362. Fumarase_classII. 1 hit.
Gene3Di1.10.275.10. 1 hit.
HAMAPiMF_00743. FumaraseC. 1 hit.
InterProiView protein in InterPro
IPR005677. Fum_hydII.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiView protein in Pfam
PF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
PRINTSiPR00149. FUMRATELYASE.
SUPFAMiSSF48557. SSF48557. 1 hit.
PROSITEiView protein in PROSITE
PS00163. FUMARATE_LYASES. 1 hit.

Sequencei

Sequence statusi: Complete.

Q03SG5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYREEADTL GTVKVPAEAL WGAQTERSRH NFTTGPTMPM AVIRALLHLK
60 70 80 90 100
RAAAIANRDL GSLREAKAQL IVQAIDQLLA LPDEKLQADF PLRVYQTGSG
110 120 130 140 150
TQTNMNVNEV IAHQATNLAP TSGILPNDDV NQSQSSNDTF PTAMAVTAYD
160 170 180 190 200
AIHQLVPVVQ RLIDELAQKQ ADYWRVVKIG RTHLQDATPL TFGQEVSGWV
210 220 230 240 250
SMLTHDLQYI QSLMPTLLEL PIGGTAVGTG LNAAPGFAVA VATQLQQAYG
260 270 280 290 300
YPFTAKTNKF FGLAAHSGLT VVHGAVKTLA GDLLKIANDV RFLASGPRAG
310 320 330 340 350
YGELNIPANE PGSSIMPGKV NPTQAEALTM AATRVMGNDV TISLAASQGN
360 370 380 390 400
FEMNVYKPVI IATFLESTTL LTGTIQGFTD RLVHGLTVNQ DRMRNLVDES
410 420 430 440 450
LMTVTALSPH IGYHASAQIA QMAEQQNLTL RAAALQSGQV TAAQFDKWVD
460
PLAMTNVDRP D
Length:461
Mass (Da):49,547
Last modified:November 14, 2006 - v1
Checksum:iC4F15E98E6480FBC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000416 Genomic DNA. Translation: ABJ63857.1.
RefSeqiWP_011667489.1. NC_008497.1.

Genome annotation databases

EnsemblBacteriaiABJ63857; ABJ63857; LVIS_0714.
GeneIDi4413917.
KEGGilbr:LVIS_0714.
PATRICifig|387344.15.peg.691.

Similar proteinsi

Entry informationi

Entry nameiQ03SG5_LACBA
AccessioniPrimary (citable) accession number: Q03SG5
Entry historyiIntegrated into UniProtKB/TrEMBL: November 14, 2006
Last sequence update: November 14, 2006
Last modified: September 27, 2017
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteomeImported