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Protein

tRNA dimethylallyltransferase

Gene

miaA

Organism
Lactobacillus brevis (strain ATCC 367 / JCM 1170)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i6A).UniRule annotation

Catalytic activityi

Dimethylallyl diphosphate + adenine(37) in tRNA = diphosphate + N(6)-dimethylallyladenine(37) in tRNA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi9 – 168ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciLBRE387344:GJ8S-992-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA dimethylallyltransferaseUniRule annotation (EC:2.5.1.75UniRule annotation)
Alternative name(s):
Dimethylallyl diphosphate:tRNA dimethylallyltransferaseUniRule annotation
Short name:
DMAPP:tRNA dimethylallyltransferaseUniRule annotation
Short name:
DMATaseUniRule annotation
Isopentenyl-diphosphate:tRNA isopentenyltransferaseUniRule annotation
Short name:
IPP transferaseUniRule annotation
Short name:
IPPTUniRule annotation
Short name:
IPTaseUniRule annotation
Gene namesi
Name:miaAUniRule annotation
Ordered Locus Names:LVIS_0992
OrganismiLactobacillus brevis (strain ATCC 367 / JCM 1170)
Taxonomic identifieri387344 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
Proteomesi
  • UP000001652 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 307307tRNA dimethylallyltransferasePRO_1000020611Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei100 – 1001Interaction with substrate tRNAUniRule annotation
Sitei124 – 1241Interaction with substrate tRNAUniRule annotation

Protein-protein interaction databases

STRINGi387344.LVIS_0992.

Structurei

3D structure databases

ProteinModelPortaliQ03RP7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni11 – 166Substrate bindingUniRule annotation
Regioni34 – 374Interaction with substrate tRNAUniRule annotation

Sequence similaritiesi

Belongs to the IPP transferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DKX. Bacteria.
COG0324. LUCA.
HOGENOMiHOG000039995.
KOiK00791.
OMAiHTRNYAK.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00185. IPP_trans. 1 hit.
InterProiIPR018022. IPP_trans.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01715. IPPT. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00174. miaA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q03RP7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKVVAVVGP TAVGKTALAI KLAQAFNGEI ISGDSMQVYR HLDIGTAKAT
60 70 80 90 100
AAEQAQAPHH LIDIQDVDQQ FTVAQFVAAA QPLIQAIHER GHLPIVAGGT
110 120 130 140 150
GFYLQALFDG LKLGADAPGD PQIREHLRQI AVEQGPQVLW QQLAAQDPVA
160 170 180 190 200
ASKIPPTNIR RTVRALEVIQ VTGQLFSHQQ NAGSQYDEYY IGLNTARPLL
210 220 230 240 250
YERINQRVDN MVQAGLLDEV RWLAKRGGAT LPAASGIGYR ELLPVLDQPE
260 270 280 290 300
KLAAAIDQIK QDSRHYAKRQ LTWFRHQTTA NWYDLVQHPE VDAQILQDVT

AWLTEKN
Length:307
Mass (Da):33,861
Last modified:November 14, 2006 - v1
Checksum:i2A6CB46D1EDC27A6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000416 Genomic DNA. Translation: ABJ64125.1.
RefSeqiWP_011667715.1. NC_008497.1.

Genome annotation databases

EnsemblBacteriaiABJ64125; ABJ64125; LVIS_0992.
GeneIDi4413933.
KEGGilbr:LVIS_0992.
PATRICi22200947. VBILacBre134470_0969.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000416 Genomic DNA. Translation: ABJ64125.1.
RefSeqiWP_011667715.1. NC_008497.1.

3D structure databases

ProteinModelPortaliQ03RP7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi387344.LVIS_0992.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABJ64125; ABJ64125; LVIS_0992.
GeneIDi4413933.
KEGGilbr:LVIS_0992.
PATRICi22200947. VBILacBre134470_0969.

Phylogenomic databases

eggNOGiENOG4105DKX. Bacteria.
COG0324. LUCA.
HOGENOMiHOG000039995.
KOiK00791.
OMAiHTRNYAK.

Enzyme and pathway databases

BioCyciLBRE387344:GJ8S-992-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00185. IPP_trans. 1 hit.
InterProiIPR018022. IPP_trans.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01715. IPPT. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00174. miaA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMIAA_LACBA
AccessioniPrimary (citable) accession number: Q03RP7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 14, 2006
Last modified: September 7, 2016
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.