Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q03RH7 (SYL_LACBA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:LVIS_1062
OrganismLactobacillus brevis (strain ATCC 367 / JCM 1170) [Complete proteome] [HAMAP]
Taxonomic identifier387344 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length809 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 809809Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009357

Regions

Motif40 – 5112"HIGH" region HAMAP-Rule MF_00049
Motif581 – 5855"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5841ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q03RH7 [UniParc].

Last modified November 14, 2006. Version 1.
Checksum: 59458C2565B219C9

FASTA80992,230
        10         20         30         40         50         60 
MAYKHQIIER KWQHYWRANH TFETSDSQTK PKYYVMDMFP YPSGQGLHVG HPEGYTATDI 

        70         80         90        100        110        120 
MARLKRMQGF NVLHPMGWDA FGLPAEQYAL KTGHNPKDFT AHNIKNFKRQ IRSLGFSYDW 

       130        140        150        160        170        180 
SREINTTDES YYKWTQWIFE QLYKKGLAYE DKIMVNWAPD FMGGTVVANE EVIDGKTERG 

       190        200        210        220        230        240 
GYPVYRVPMR QWVLKITAYA DRLIDDLDDL DWPDSIKEMQ RNWIGRSEGA SVFFPVDGQA 

       250        260        270        280        290        300 
DTKIEVYTTR PDTLFGATYM VLAPEHAAVA KITTPEQADA VKAYQEAIES KSDLERTDLN 

       310        320        330        340        350        360 
KDKTGVFTGA YGINPLSGKK LPIWIGDYVL SSYGTGAIMA VPAHDERDHE FATKFNLPIT 

       370        380        390        400        410        420 
QVIEGDADTD ITEAAYTDDG KHINSDFMNG MDKQTAISAA IDWLTEHDAG HKQVNFRLRD 

       430        440        450        460        470        480 
WIFSRQRYWG EPIPVIHWEN GETTLVPEDE LPLKLPAAKQ LEPSGTGESP LANLDDWVNV 

       490        500        510        520        530        540 
VDENGRKGKR ETNTMPQWAG SSWYFLRYVD PTNDQAIADP EKLKYWMPVD LYIGGAEHAV 

       550        560        570        580        590        600 
LHLLYARFWH KFLYDLGVVP TKEPFQKLVN QGMILGTNHE KMSKSKGNVI NPDEIVEKHG 

       610        620        630        640        650        660 
ADTLRLYEMF MGPLEASKPW STEGINGSRR WLDRVWRLLI DDNNRLRDRV TMINDGALDK 

       670        680        690        700        710        720 
IYNQTVKTVT EDLEAMRFNV AISQLMVFVN EAYKVDSLPM IYMEGFVKLI SPIVPHIAEE 

       730        740        750        760        770        780 
LWSLLGHDET IAYAKWPTYD AKQLVEDVVE LVVQVNGKVR AHLKVAKDAT KDAIEAAALA 

       790        800 
DETVQVHTDG KTVRKVIVVP GKLVNIVAN 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000416 Genomic DNA. Translation: ABJ64195.1.
RefSeqYP_795226.1. NC_008497.1.

3D structure databases

ProteinModelPortalQ03RH7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING387344.LVIS_1062.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABJ64195; ABJ64195; LVIS_1062.
GeneID4414232.
KEGGlbr:LVIS_1062.
PATRIC22201085. VBILacBre134470_1038.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycLBRE387344:GJ8S-1062-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_LACBA
AccessionPrimary (citable) accession number: Q03RH7
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 14, 2006
Last modified: April 16, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries