Q03QS8 (SYP_LACBA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	LVIS_1342

Organism

Lactobacillus brevis (strain ATCC 367 / JCM 1170) [Complete proteome] [HAMAP]

Taxonomic identifier

387344 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Lactobacillaceae › Lactobacillus › Lactobacillus brevis

Protein attributes

Sequence length	569 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity. HAMAP-Rule MF_01569
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP-Rule MF_01569
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_01569
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_01569.
Domain	Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity. HAMAP-Rule MF_01569
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro regulation of translational fidelity Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aminoacyl-tRNA editing activity Inferred from electronic annotation. Source: InterPro proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 569	569	Proline--tRNA ligase HAMAP-Rule MF_01569		PRO_0000288334

Sequences

	Sequence		Length	Mass (Da)
	Q03QS8 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: AE3D88AC69912694	FASTA	569	63,430
	10 20 30 40 50 60 MKQSQLLIPT LKEVPNDAEA LSHQMMLRAG YIRQVTAGMY AYLPLAFRVL TNIETIIREE 70 80 90 100 110 120 MEKINAVEML MPAVLPASLW QESGRYETYG PNLFKFKNRH DSDFILAPTH EETFTMLVRD 130 140 150 160 170 180 AIKSYKRLPL VMYQIQPKYR DEDRPRYGLL RGREFIMKDA YSFSLSDEDL DRIYNQMEQA 190 200 210 220 230 240 YENIFDRIGL NYRAIVGDGG AMGGKDSKEF SAIAPVGEDT IVYSDSSDYA ANLEMAKSLF 250 260 270 280 290 300 VSKKSHAQLA DLEKIATPGV HSIAELAEFL DVKPAALVKS MLYIADDQPV MVLVRGDHEV 310 320 330 340 350 360 NETKLKNYLN ADFLNPATPE DAQKYLGANF GSLGPVGVSE DVKILADQYV GDMVNVAVGA 370 380 390 400 410 420 DEDEHHYLNA NLDRDFRVDA FADLREVQPG DLSPDGSGVL KFTKGIEIGH IFKLGTRYSD 430 440 450 460 470 480 ALGATVLDEG GRQKPVVMGS YGIGVSRLLS AIAEQQADDK GLVWPRNIAP FDIHLVPVNL 490 500 510 520 530 540 KKDDQAQLTS ELEEQLTAKG YRILTDDRKE RPGVKFADSD LMGIPVRITI GKKAGEGIVE 550 560 IKIRKTGETV EVIKDEVAST VEILFKDID

References

[1]

"Comparative genomics of the lactic acid bacteria." Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V., Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V., Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M., Hawkins T. , Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A., Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W., Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y., Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R., O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T., Richardson P., Kozyavkin S., Weimer B.C., Mills D.A. Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 367 / JCM 1170.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000416 Genomic DNA. Translation: ABJ64444.1.
RefSeq	YP_795475.1. NC_008497.1.
3D structure databases
ProteinModelPortal	Q03QS8.
SMR	Q03QS8. Positions 1-564.
ModBase	Search...
Protein-protein interaction databases
STRING	387344.LVIS_1342.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABJ64444; ABJ64444; LVIS_1342.
GeneID	4414561.
KEGG	lbr:LVIS_1342.
PATRIC	22201613. VBILacBre134470_1276.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0442.
HOGENOM	HOG000076893.
KO	K01881.
OMA	IQPAELW.
ProtClustDB	PRK09194.
Enzyme and pathway databases
BioCyc	LBRE387344:GJ8S-1342-MONOMER.
Family and domain databases
Gene3D	3.40.50.800. 1 hit. 3.90.960.10. 1 hit.
HAMAP	MF_01569. Pro_tRNA_synth_type1.
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-ligase_IIa. IPR004500. Pro-tRNA-synth_IIa_bac-type. IPR023717. Pro-tRNA-Synthase_IIa_type1. IPR007214. YbaK/aa-tRNA-synth-assoc-dom. [Graphical view]
PANTHER	PTHR11451:SF3. PTHR11451:SF3. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF04073. YbaK. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF55826. YbaK/aa-tRNA-synth-assoc-reg. 1 hit.
TIGRFAMs	TIGR00409. proS_fam_II. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_LACBA

Accession

Primary (citable) accession number: Q03QS8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	November 14, 2006
Last modified:	May 29, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program