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Q03QI3

- SYI_LACBA

UniProt

Q03QI3 - SYI_LACBA

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Lactobacillus brevis (strain ATCC 367 / JCM 1170)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 1 (14 Nov 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei556 – 5561Aminoacyl-adenylateUniRule annotation
    Binding sitei600 – 6001ATPUniRule annotation
    Metal bindingi895 – 8951ZincUniRule annotation
    Metal bindingi898 – 8981ZincUniRule annotation
    Metal bindingi915 – 9151ZincUniRule annotation
    Metal bindingi918 – 9181ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciLBRE387344:GJ8S-1441-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:LVIS_1441
    OrganismiLactobacillus brevis (strain ATCC 367 / JCM 1170)
    Taxonomic identifieri387344 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
    ProteomesiUP000001652: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 937937Isoleucine--tRNA ligasePRO_1000022081Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi387344.LVIS_1441.

    Structurei

    3D structure databases

    ProteinModelPortaliQ03QI3.
    SMRiQ03QI3. Positions 1-924.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 6711"HIGH" regionAdd
    BLAST
    Motifi597 – 6015"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q03QI3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRVKDTLNLG KTKFKMRGNL PVKEVDRQKA WAENKMYEAR QKLNEGKPTF    50
    ILHDGPPYAN GPIHMGHALN KISKDIIVRY KSMSGFRAPY VPGWDTHGLP 100
    IEQQLTKAGY DRKKMSTAEF RDLCREYALK QVDQQREGFK RLGVSAEWDN 150
    PYLTLKPEFE AAEVRVFGEM AKRGLIYKGK KPVYWSWSSE SAMAEAEVEY 200
    HDVTSPSAFY GEQVVDGKGV LDTDTYLVVW TTTPWTIPGS EGITIDAGIE 250
    YAVVKPANDD RKFVLAADLV DQNAEMFGWE DVQVIKTVMG QDLDNVTAQH 300
    PFIADRKLVV MLGDFVTTES GTGLVHTAPG LGEDDFNVGA LYHLPVLVPV 350
    DDKGYMTAEA GADFAGVFYE DANQIALDKL KAANALLKYM PYEHSYPFDW 400
    RTKKPVIFRA TPQWFASVDK IRDEILAQLK DVKFQPDWGQ RRLANMIKDR 450
    GDWVISRQRV WGVPLPIFYG EDGEAIITPE TVDHVADLFA EYGSNIWFKR 500
    EAKDLLPDGF TSEHSPNGEF TKETDIMDVW FDSGTSHQGV LAERDYLDFP 550
    ADLYLEGSDQ YRGWFNSSLI TSVAATGKAP YKQVVSQGFT LDKDGHKMSK 600
    SLGNTIAPDE IISKMGADIV RLWVTSVDSS ADVRVSTEAF VKISDSYKKL 650
    RNTMRYLLAN TSDFDPQTDA IAVDDLQPVD RHMLYQLNEF AKSVRAHYDH 700
    YDFLNIYKEL INFVVSDLSA FYLDFAKDIL YIEAADSPVR RSMQTVFYQI 750
    AVSLTKLITP ILPHTAEEIW DFLKEPEDFV QLAEMPAVLE LTDANEQPDD 800
    GHQSAWDHFM TLRSHVLKAL EEARDAKLIG KAAEAHLDLY VDEETKRLLD 850
    QLNVNVQQIL LVSGLDVASL DQAPADALTF DHLAVKVTPA AGEVCDRCRL 900
    TKEDVGSDSA YPHFCARCAA IVRQNFPETA TEGFDEN 937
    Length:937
    Mass (Da):105,621
    Last modified:November 14, 2006 - v1
    Checksum:iE4C032D9680F36D3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000416 Genomic DNA. Translation: ABJ64539.1.
    RefSeqiYP_795570.1. NC_008497.1.

    Genome annotation databases

    EnsemblBacteriaiABJ64539; ABJ64539; LVIS_1441.
    GeneIDi4413669.
    KEGGilbr:LVIS_1441.
    PATRICi22201819. VBILacBre134470_1378.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000416 Genomic DNA. Translation: ABJ64539.1 .
    RefSeqi YP_795570.1. NC_008497.1.

    3D structure databases

    ProteinModelPortali Q03QI3.
    SMRi Q03QI3. Positions 1-924.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 387344.LVIS_1441.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABJ64539 ; ABJ64539 ; LVIS_1441 .
    GeneIDi 4413669.
    KEGGi lbr:LVIS_1441.
    PATRICi 22201819. VBILacBre134470_1378.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci LBRE387344:GJ8S-1441-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 367 / JCM 1170.

    Entry informationi

    Entry nameiSYI_LACBA
    AccessioniPrimary (citable) accession number: Q03QI3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: November 14, 2006
    Last modified: October 1, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3