Reviewed, UniProtKB/Swiss-Prot Q03PZ8 (ASNA_LACBA)

Names and origin

Protein names

Recommended name:
    Aspartate--ammonia ligase
    EC=6.3.1.1
Alternative name(s):
    Asparagine synthetase A

Gene names

Name:	asnA
Ordered Locus Names:	LVIS_1649

Organism

Lactobacillus brevis (strain ATCC 367 / JCM 1170) [Complete proteome] [HAMAP]

Taxonomic identifier

387344 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP MF_00555
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP MF_00555
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: HAMAP tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 335	335	Aspartate--ammonia ligase HAMAP MF_00555		PRO_1000017951

Sequences

	Sequence		Length	Mass (Da)
	Q03PZ8-1 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: 5E32D9D3E85A1B6C	FASTA	335	38,743
	10 20 30 40 50 60 MHLIIPDSYD PKLSVKDTQR AIRYIRETFQ DEFGAQLNLS RLSAPMFVEK STGLNDNLNG 70 80 90 100 110 120 IEQPVSFTMQ DMGDSQIEIV HSLAKWKRVA LKRYGFNLHE GLYTNMNAIR KDEDLDNFHS 130 140 150 160 170 180 AYVDQWDWEK VITKEERTLD TLKQTVRQIF KVIKHMEHEV WYKFPEAVHH LPDKIHFITT 190 200 210 220 230 240 QELEDRFPDL TPRERENAIT KELGCVFLMQ IGGALKSGKR HDGRAPDYDD WQLNGDILFW 250 260 270 280 290 300 YEPLKQAIEI SSMGIRVDAE SMQRQLKIAD AEDRLSLPYH QMVLHEEVPF TIGGGIGQSR 310 320 330 LCMLLLGKAH VGEVQAALWP QAMIDQCEAA NIHLL

References

[1]

"Comparative genomics of the lactic acid bacteria." Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V., Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V., Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M., Hawkins T. , Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A., Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W., Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y., Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R., O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T., Richardson P., Kozyavkin S., Weimer B.C., Mills D.A. Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006) [PubMed: 17030793] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000416 Genomic DNA. Translation: ABJ64724.1.
RefSeq	YP_795755.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q03PZ8.
Genome annotation databases
GeneID	4413731.
GenomeReviews	Gene locus LVIS_1649 in contig CP000416_GR.
KEGG	lbr:LVIS_1649.
NMPDR	fig|387344.8.peg.1535.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q03PZ8.
OMA	LNDNLNG.
Family and domain databases
HAMAP	MF_00555. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II_cons-reg. IPR004618. AsnA. [Graphical view]
Pfam	PF03590. AsnA. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
ProDom	PD024629. AsnA. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00669. asnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNA_LACBA

Accession

Primary (citable) accession number: Q03PZ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 14, 2006
Last modified:	November 3, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)