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Q03MJ2 (SYL_STRTD) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:STER_0268
OrganismStreptococcus thermophilus (strain ATCC BAA-491 / LMD-9) [Complete proteome] [HAMAP]
Taxonomic identifier322159 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length833 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 833833Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009452

Regions

Motif41 – 5212"HIGH" region HAMAP-Rule MF_00049
Motif610 – 6145"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6131ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q03MJ2 [UniParc].

Last modified November 14, 2006. Version 1.
Checksum: 44462D3B0B2BE70B

FASTA83394,422
        10         20         30         40         50         60 
MSFYNHKEIE PKWQKYWADH HTFKTGTDAS KPKFYALDMF PYPSGAGLHV GHPEGYTATD 

        70         80         90        100        110        120 
ILSRFKRAQG YNVLHPMGWD AFGLPAEQYA MDTGNDPADF TAENIANFKR QINALGFSYD 

       130        140        150        160        170        180 
WDREINTTDP NYYKWTQWIF TKLYEKGLAY EAEVPVNWVE ELGTAIANEE VLPDGTSERG 

       190        200        210        220        230        240 
GYPVVRKPMR QWMLKITAYA ERLLNDLDEL DWPESIKDMQ RNWIGKSTGA NVTFKVKGTD 

       250        260        270        280        290        300 
KEFTVFTTRP DTLFGATFTV LAPEHDLVDA ITSPEQAEAV ANYKHQASLK SDLARTDLAK 

       310        320        330        340        350        360 
EKTGVWTGAY AINPVNGREI PIWIADYVLA SYGTGAVMAV PAHDERDWEF AKQFGLPIVE 

       370        380        390        400        410        420 
VLEGGNVEEA AYTEDGPHVN SDFLNGLNKE EAIAKIVAWL EEKGFGQEKI TYRLRDWLFS 

       430        440        450        460        470        480 
RQRYWGEPIP IIHWEDGTST AVPESELPLV LPVTKDIRPS GTGESPLANL TDWLEVTRED 

       490        500        510        520        530        540 
GVKGRRETNT MPQWAGSSWY YLRYIDPHNT EKLADEDLLK QWLPVDIYVG GAEHAVLHLL 

       550        560        570        580        590        600 
YARFWHKFLY DLGVVPTKEP FQKLFNQGMI LGTSYRDHRG ALVATDKVEK RDGSFFHVET 

       610        620        630        640        650        660 
GEELEQAPAK MSKSLKNVVN PDDVVEQYGA DTLRVYEMFM GPLDASIAWS EEGLEGSRKF 

       670        680        690        700        710        720 
LDRVYRLITS KEIVAENNGG LDKVYNETVK SVTEQIELMK FNTAIAQLMV FVNAANKEDK 

       730        740        750        760        770        780 
LYVDYAKGFV QLIAPFAPHL AEELWQTLTA TGESISYVAW PTWDESKLVE DEIEIVVQIK 

       790        800        810        820        830 
GKVRAKLMVA KDLSREELQE VALADEKVKA EIDGKEIVKV IAVPNKLVNI VVK 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000419 Genomic DNA. Translation: ABJ65580.1.
RefSeqYP_819776.1. NC_008532.1.

3D structure databases

ProteinModelPortalQ03MJ2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING322159.STER_0268.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABJ65580; ABJ65580; STER_0268.
GeneID4438392.
KEGGste:STER_0268.
PATRIC19797727. VBIStrThe129203_0234.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycSTHE322159:GJ9F-268-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_STRTD
AccessionPrimary (citable) accession number: Q03MJ2
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 14, 2006
Last modified: April 16, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries