Q03M88 (IF2_STRTD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Translation initiation factor IF-2

Gene names

Name:	infB
Ordered Locus Names:	STER_0383

Organism

Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9) [Complete proteome] [HAMAP]

Taxonomic identifier

322159 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus › Streptococcus thermophilus

Protein attributes

Sequence length	943 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex By similarity. HAMAP-Rule MF_00100
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the IF-2 family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Initiation factor
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	GTP catabolic process Inferred from electronic annotation. Source: GOC small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: HAMAP translation initiation factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 943	943	Translation initiation factor IF-2 HAMAP-Rule MF_00100		PRO_1000008357
Regions
	Nucleotide binding	454 – 461	8	GTP By similarity
	Nucleotide binding	500 – 504	5	GTP By similarity
	Nucleotide binding	554 – 557	4	GTP By similarity
	Region	448 – 596	149	G-domain HAMAP-Rule MF_00100

Sequences

	Sequence		Length	Mass (Da)
	Q03M88 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: E66470CAF95BBC1B	FASTA	943	103,824
	10 20 30 40 50 60 MSKKRLYEIA KEVGVESKVI VAKAQELGLS VKSHSSSVEE ADANRITSSL KAGTAKDESK 70 80 90 100 110 120 PAPKATPTPK EEKVEPKVDK ASVAKSAPAK ETSKAEVKEA SVALKKPKSR NFKAEREARA 130 140 150 160 170 180 KAEAERRKNG GGRDNRNRNQ QGNDQGKRHN NDRRNQKGNG QGDHNKGNRD NSTNHDRNFQ 190 200 210 220 230 240 GKLRNDQNQN NRRDNARNNQ AGPRIDLKAR AAALKAEQNA EYSRQSETRF REEKAAEQRR 250 260 270 280 290 300 AKEQEKARKE KQQAEVAVQK AAAETKPAPK PAPVAPQSAP TAQVQDTRRK KVRPNKSRDN 310 320 330 340 350 360 HRVNEDGPKQ TRNNKWNNQN QVRNQRNSNW NKNKNKKGKN NRGNSAPKLV TERKFHELPK 370 380 390 400 410 420 EFEYTEGMTV AEIAKRIKRE PAEIVKKLFM MGVMATQNQS LDGDTIELLM VDYGIEATKK 430 440 450 460 470 480 EEVDNADIER FFVDEDYLNK DAMVERAPVV TIMGHVDHGK TTLLDTLRNS RVATGEAGGI 490 500 510 520 530 540 TQHIGAYQIE EGGKKITFLD TPGHAAFTSM RARGASVTDI TVLIVAADDG VMPQTIEAIN 550 560 570 580 590 600 HSKAAGVPII VAINKIDKPD ANPERVIGEL AEHGVISTAW GGDSEFVEIS AKFGQNIEEL 610 620 630 640 650 660 LETILLVAEV EELKADPTVR AIGTVIEARL DKGKGAVATL LVQQGTLNVQ DPIVVGNTFG 670 680 690 700 710 720 RVRAMTNDLG RRIKTAGPSA PVSITGLNEA PMAGDHFAVY EDEKAARAAG EERAKRALMK 730 740 750 760 770 780 QRQQTHRVSL DNLFDTLKAG EMKTVNVIIK ADVQGSVEAL AASLLKIDVE GVRVNVVHSA 790 800 810 820 830 840 VGAINESDIT LAEASDAVVI GFNVRPTPQA RQQAETDEVE IRLHSIIYKV IEEIEDAMKG 850 860 870 880 890 900 MLDPEFEEKI IGEAVIRETF KVSKVGTIGG FMVTNGKITR DSSARVIRDG VVIFDGKLAS 910 920 930 940 LKHYKDDVKE VGNAQEGGLT IENYNDIKVD DVIEAYIMEE IKR

References

[1]

"Comparative genomics of the lactic acid bacteria." Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V., Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V., Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M., Hawkins T. , Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A., Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W., Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y., Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R., O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T., Richardson P., Kozyavkin S., Weimer B.C., Mills D.A. Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-491 / LMD-9.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000419 Genomic DNA. Translation: ABJ65684.1.
RefSeq	YP_819880.1. NC_008532.1.
3D structure databases
ProteinModelPortal	Q03M88.
SMR	Q03M88. Positions 718-837, 846-943.
ModBase	Search...
Protein-protein interaction databases
STRING	322159.STER_0383.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABJ65684; ABJ65684; STER_0383.
GeneID	4437940.
KEGG	ste:STER_0383.
PATRIC	19797975. VBIStrThe129203_0356.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0532.
HOGENOM	HOG000076906.
KO	K02519.
OMA	EEIEDAM.
ProtClustDB	PRK05306.
Family and domain databases
Gene3D	3.40.50.10050. 1 hit.
HAMAP	MF_00100_B. IF-2_B.
InterPro	IPR000795. EF_GTP-bd_dom. IPR006847. IF2_N. IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. IPR000178. TF_IF2_bacterial-like. IPR015760. TIF_IF2. IPR023115. TIF_IF2_dom3. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
PANTHER	PTHR23115:SF41. PTHR23115:SF41. 1 hit.
Pfam	PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. PF11987. IF-2. 1 hit. PF04760. IF2_N. 2 hits. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SUPFAM	SSF52156. TIF_IF2_dom3. 1 hit. SSF50447. Translat_factor. 2 hits.
TIGRFAMs	TIGR00487. IF-2. 1 hit. TIGR00231. small_GTP. 1 hit.
PROSITE	PS01176. IF2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IF2_STRTD

Accession

Primary (citable) accession number: Q03M88

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 14, 2006
Last modified:	May 1, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program