Q03LY7 (KHSE_STRTD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homoserine kinase
Short name=HK
Short name=HSK
EC=2.7.1.39

Gene names

Name:	thrB
Ordered Locus Names:	STER_0507

Organism

Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9) [Complete proteome] [HAMAP]

Taxonomic identifier

322159 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate By similarity. HAMAP MF_00384
Catalytic activity	ATP + L-homoserine = ADP + O-phospho-L-homoserine. HAMAP MF_00384
Pathway	Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5. HAMAP MF_00384
Subcellular location	Cytoplasm Potential HAMAP MF_00384.
Sequence similarities	Belongs to the GHMP kinase family. Homoserine kinase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Threonine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW homoserine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 286	286	Homoserine kinase HAMAP MF_00384		PRO_1000049180
Regions
	Nucleotide binding	78 – 88	11	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q03LY7 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: 05011F71AB46A369	FASTA	286	30,193
	10 20 30 40 50 60 MKITVPATSA NIGPGFDSVG VAVSKYLTIE VLEPADVWFI EHDLGDIPSD ENNLLISTAL 70 80 90 100 110 120 QVKSDLQPHK LVMTSDIPLA RGLGSSSSVI VAGIELANQL ADLKLSDDDK LDIATKIEGH 130 140 150 160 170 180 PDNVAPAIFG NLVVASYVDE HVNSIVTEFP ECAFVAFIPS YELKTSESRG ALPSDLSYKD 190 200 210 220 230 240 AVAASSIANV AIAALFAGDL VKAGRAIQGD MFHERYRQKL VKEFATIKEL SGQYGAYATY 250 260 270 280 LSGAGPTVMT LTPNDQAEAL KTAIDGLGLD GETLILSVDK GGVVVD

References

[1]

"Comparative genomics of the lactic acid bacteria." Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V., Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V., Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M., Hawkins T. , Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A., Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W., Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y., Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R., O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T., Richardson P., Kozyavkin S., Weimer B.C., Mills D.A. Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006) [PubMed: 17030793] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-491 / LMD-9.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000419 Genomic DNA. Translation: ABJ65785.1.
RefSeq	YP_819981.1. NC_008532.1.
3D structure databases
ProteinModelPortal	Q03LY7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q03LY7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTRT00000060410; EBSTRP00000056066; EBSTRG00000060400.
GeneID	4437223.
GenomeReviews	Gene locus STER_0507 in contig CP000419_GR.
KEGG	ste:STER_0507.
PATRIC	19798241. VBIStrThe129203_0478.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0083.
GeneTree	EBGT00050000027810.
HOGENOM	HBG646290.
OMA	NNALMST.
ProtClustDB	PRK01212.
Enzyme and pathway databases
BioCyc	STHE322159:STER_0507-MONOMER.
Family and domain databases
HAMAP	MF_00384. Homoser_kinase. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR006203. GHMP_knse_ATP-bd_CS. IPR000870. Homoserine_kinase. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00872.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF000676. Homoser_kin. 1 hit.
PRINTS	PR00958. HOMSERKINASE.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00191. ThrB. 1 hit.
PROSITE	PS00627. GHMP_KINASES_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KHSE_STRTD

Accession

Primary (citable) accession number: Q03LY7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 14, 2006
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program