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Q03LY1 (Q03LY1_STRTD) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Valine--tRNA ligase HAMAP-Rule MF_02004
EC=6.1.1.9 HAMAP-Rule MF_02004
Alternative name(s):
Valyl-tRNA synthetase HAMAP-Rule MF_02004
Gene names
Name:valS HAMAP-Rule MF_02004
Ordered Locus Names:STER_0513 EMBL ABJ65791.1
OrganismStreptococcus thermophilus (strain ATCC BAA-491 / LMD-9) [Complete proteome] [HAMAP] EMBL ABJ65791.1
Taxonomic identifier322159 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus ›

Protein attributes

Sequence length883 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a "posttransfer" editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner By similarity. HAMAP-Rule MF_02004 SAAS SAAS019499

Catalytic activity

ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val). HAMAP-Rule MF_02004 SAAS SAAS019499

Subunit structure

Monomer By similarity. HAMAP-Rule MF_02004 SAAS SAAS019499

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_02004 SAAS SAAS019499.

Domain

The C-terminal coiled-coil domain is crucial for aminoacylation activity By similarity. HAMAP-Rule MF_02004

ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site By similarity. HAMAP-Rule MF_02004

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily. HAMAP-Rule MF_02004

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Coiled coil809 – 84436 By similarity HAMAP-Rule MF_02004
Motif46 – 5611"HIGH" region By similarity HAMAP-Rule MF_02004
Motif520 – 5245"KMSKS" region By similarity HAMAP-Rule MF_02004

Sites

Binding site5231ATP By similarity HAMAP-Rule MF_02004

Sequences

Sequence LengthMass (Da)Tools
Q03LY1 [UniParc].

Last modified November 14, 2006. Version 1.
Checksum: 666AC6A264D9B6A7

FASTA883101,218
        10         20         30         40         50         60 
MSKELSPKYN PAEVEAGRYQ KWLDEDVFKP SGDKKAKPYS IVIPPPNVTG KLHLGHAWDT 

        70         80         90        100        110        120 
TLQDIIIRQK RMQGFDTLWL PGMDHAGIAT QAKVEARLAE SGISRYDLGR EKFLDKVWEW 

       130        140        150        160        170        180 
KDEYAATIKE QWGKMGLSVD YSRERFTLDE GLSKAVRKVF VELYKKGWIY RGEFIINWDP 

       190        200        210        220        230        240 
KARTALSDIE VIHKDVEGAF YHMNYMLEDG SRALEVATTR PETMFGDTAV AVNPNDDRYK 

       250        260        270        280        290        300 
DLIGKNVILP ILNKPIPIVG DEHADPEFGT GVVKITPAHD PNDFLVGQRH NLPQVNVMND 

       310        320        330        340        350        360 
DGTMNELAGE FNGMDRFEAR KAVVKKLEEI GALVEIEKMT HSVGHSERTG VPVEPRLSTQ 

       370        380        390        400        410        420 
WFVKMDQLAK NAIANQDTDD KVDFYPPRFN DTFLQWMENV HDWVISRQLW WGHQIPAWYN 

       430        440        450        460        470        480 
TEGEIYVGEE APEGDGWKQD EDVLDTWFSS ALWPFSTMGW PDVDSEDFKR YFPTSTLVTG 

       490        500        510        520        530        540 
YDIIFFWVSR MIFQSLEFTG RQPFKNVLIH GLIRDEQGRK MSKSLGNGID PMDVIEKYGA 

       550        560        570        580        590        600 
DALRWFLSNG SAPGQDVRFS YEKMDASWNF INKIWNISRY ILMNNEGLTL DVARENVAKV 

       610        620        630        640        650        660 
AAGQAGNVTD RWILHNLNET IGKVTENFDK FEFGVAGHIL YNFIWDEFAD WYVELTKEVL 

       670        680        690        700        710        720 
YSDNEDEKVI TRSVLLYTLD QILRLLHPIM PFVTEEIFGQ ISEGSIVTAE YPVVRPEFEN 

       730        740        750        760        770        780 
EEAAAGVEAL KDVIRSVRNS RAEVNVAPSK PITILIKTSD SKLDAFFNDN INYIKRFTNP 

       790        800        810        820        830        840 
EHLEIAADVE VPDLVMSSII TGAEIYLPLA DLLNVEEELA RLEKELAKWQ KELDMVGKKL 

       850        860        870        880 
SNERFVANAK PEVVQKERDK QKDYQAKYDA IVVRINEMKK LVK

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000419 Genomic DNA. Translation: ABJ65791.1.
RefSeqYP_819987.1. NC_008532.1.

3D structure databases

ProteinModelPortalQ03LY1.
ModBaseSearch...

Protein-protein interaction databases

STRING322159.STER_0513.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABJ65791; ABJ65791; STER_0513.
GeneID4437229.
KEGGste:STER_0513.
PATRIC19798253. VBIStrThe129203_0484.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0525.
HOGENOMHOG000020093.
KOK01873.
OMADPLEMIE.
ProtClustDBPRK05729.

Family and domain databases

Gene3D1.10.287.380. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02004. Val_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR014729. Rossmann-like_a/b/a_fold.
IPR010978. tRNA-bd_arm.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR019499. Val-tRNA_synth_tRNA-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR002303. Valyl-tRNA_ligase.
[Graphical view]
PANTHERPTHR11946:SF5. PTHR11946:SF5. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF10458. Val_tRNA-synt_C. 1 hit.
[Graphical view]
PRINTSPR00986. TRNASYNTHVAL.
SUPFAMSSF46589. tRNA_binding_arm. 1 hit.
SSF47323. tRNAsyn_1a_bind. 1 hit.
SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMsTIGR00422. valS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameQ03LY1_STRTD
AccessionPrimary (citable) accession number: Q03LY1
Entry history
Integrated into UniProtKB/TrEMBL: November 14, 2006
Last sequence update: November 14, 2006
Last modified: May 1, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info