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Protein

Thymidylate synthase

Gene

thyA

Organism
Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei161NucleophileUniRule annotation1
Binding sitei1885,10-methylenetetrahydrofolateUniRule annotation1
Binding sitei196dUMPUniRule annotation1
Binding sitei2855,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi140 – 141dUMP; shared with dimeric partnerUniRule annotation2
Nucleotide bindingi185 – 188dUMPUniRule annotation4
Nucleotide bindingi226 – 228dUMPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processNucleotide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00575

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
Ordered Locus Names:STER_0622
OrganismiStreptococcus thermophilus (strain ATCC BAA-491 / LMD-9)
Taxonomic identifieri322159 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000006971 – 286Thymidylate synthaseAdd BLAST286

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ03LN3
SMRiQ03LN3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000257898
KOiK00560
OMAiWNEWEVG

Family and domain databases

CDDicd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Di3.30.572.10, 1 hit
HAMAPiMF_00008 Thymidy_synth_bact, 1 hit
InterProiView protein in InterPro
IPR023451 Thymidate_synth/dCMP_Mease
IPR036926 Thymidate_synth/dCMP_Mease_sf
IPR000398 Thymidylate_synthase
IPR020940 Thymidylate_synthase_AS
PfamiView protein in Pfam
PF00303 Thymidylat_synt, 1 hit
PRINTSiPR00108 THYMDSNTHASE
SUPFAMiSSF55831 SSF55831, 1 hit
PROSITEiView protein in PROSITE
PS00091 THYMIDYLATE_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q03LN3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKADTIFKE NITKIMEEGV WSEQARPKYK DGTTANSKYI TGSFAEYDLS
60 70 80 90 100
KGEFPITTLR PIAIKSAIKE VFWIYQDQTN SLDVLEDKYN VHYWNDWEVE
110 120 130 140 150
GVPANNGDKR SIGQRYGAVV KKHDIINRLL AQLEANPWNR RNVISLWDYE
160 170 180 190 200
AFEETAGLQP CAFQTMFDVR RVGEDVYLDA TLTQRSNDML VAHHINAMQY
210 220 230 240 250
VALQMMIAKH FGWKVGKFFY FINNLHIYDN QFEQAEELLK RQPSDCQPRL
260 270 280
VLNVPDETNF FDIKPEDFEL VDYDPVKPQL KFDLAI
Length:286
Mass (Da):33,370
Last modified:November 14, 2006 - v1
Checksum:iD1BF1A1CBCF80690
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000419 Genomic DNA Translation: ABJ65889.1
RefSeqiWP_002950145.1, NC_008532.1

Genome annotation databases

EnsemblBacteriaiABJ65889; ABJ65889; STER_0622
KEGGiste:STER_0622

Similar proteinsi

Entry informationi

Entry nameiTYSY_STRTD
AccessioniPrimary (citable) accession number: Q03LN3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 14, 2006
Last modified: March 28, 2018
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

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