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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei554 – 5541Aminoacyl-adenylateUniRule annotation
Binding sitei598 – 5981ATPUniRule annotation
Metal bindingi888 – 8881ZincUniRule annotation
Metal bindingi891 – 8911ZincUniRule annotation
Metal bindingi908 – 9081ZincUniRule annotation
Metal bindingi911 – 9111ZincUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSTHE322159:GJ9F-783-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:STER_0783
OrganismiStreptococcus thermophilus (strain ATCC BAA-491 / LMD-9)
Taxonomic identifieri322159 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 929929Isoleucine--tRNA ligasePRO_1000022133Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ03L88.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi595 – 5995"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03L88-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLKETLNLG KTAFPMRAGL PNKEPIWQKE WEEAKVYQRR QELNQGKPHF
60 70 80 90 100
TLHDGPPYAN GNIHVGHAMN KISKDIIVRS KSMSGFYAPF IPGWDTHGLP
110 120 130 140 150
IEQVLAKQGV KRKELDRAEY LKMCRDYALS QVDKQREDFK RLGVSADWEN
160 170 180 190 200
PYVTLTPDYE AAQIRVFGEM AKKGYIYQGA KPVYWSWSSE SALAEAEIEY
210 220 230 240 250
HDLVSTSLYY ANKVKDGKGV LDTDTYIVVW TTTPFTVTAS RGLAVGADIE
260 270 280 290 300
YVLVKPAGET RKFIVASELL NSLSEKFAWE EVEVLNTYRG DELNQIVTEH
310 320 330 340 350
PWDSEVDELV ILGEHVTTDS GTGIVHTAPG FGEDDYNVGV ANGLEVVVTV
360 370 380 390 400
NERGIMMENA GPDFAGKFYD KVAPIVMEKL GDLLLAKEEI SHSYPFDWRT
410 420 430 440 450
KKPIIWRAVP QWFASVSKFR QEILDEIEKV KFHSEWGKVR LYNMIRDRGD
460 470 480 490 500
WVISRQRAWG VPLPIFYAED RTPIMTEETI EHVAKLFEEH GSVIWWERDA
510 520 530 540 550
KDLLPEGFTH PGSPNGEFTK ENDIMDVWFD SGSSWNGVVV NRPELTYPAD
560 570 580 590 600
LYLEGSDQYR GWFNSSLITS VANHGVAPYK QLLSQGFALD GKGEKMSKSL
610 620 630 640 650
GNTIAPSDVE KQFGAEILRL WVTSVDTSND VRISMDILSQ VSESYRKIRN
660 670 680 690 700
TLRFLIANTS DFNPTTDAVA FEDLRSVDQY MTIRFNQLVK NIRDAYENFE
710 720 730 740 750
FLTIYKSLVN FINVELSAFY LDFAKDVVYI ESAKSLERRQ MQTVFYDILV
760 770 780 790 800
KITKLLTPIL PHTAEEIWSY LEFENEDYVQ LSELPEAEDF ANQDALLEKW
810 820 830 840 850
NAFMDFRGKA QKALEEARNE KVIGKSLEAH LTIYPDAEVK ELLESLNTNL
860 870 880 890 900
AQLLIVSALT IAEGDVPESA VRFQGVSFTV ERAEGEVCDR CRRIDPTTKE
910 920
RSYNATICNH CASIIEENFA EVVAEGFEV
Length:929
Mass (Da):105,808
Last modified:November 14, 2006 - v1
Checksum:iBEEE99391867442B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000419 Genomic DNA. Translation: ABJ66034.1.
RefSeqiWP_011681009.1. NC_008532.1.

Genome annotation databases

EnsemblBacteriaiABJ66034; ABJ66034; STER_0783.
KEGGiste:STER_0783.
PATRICi19798829. VBIStrThe129203_0767.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000419 Genomic DNA. Translation: ABJ66034.1.
RefSeqiWP_011681009.1. NC_008532.1.

3D structure databases

ProteinModelPortaliQ03L88.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABJ66034; ABJ66034; STER_0783.
KEGGiste:STER_0783.
PATRICi19798829. VBIStrThe129203_0767.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciSTHE322159:GJ9F-783-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-491 / LMD-9.

Entry informationi

Entry nameiSYI_STRTD
AccessioniPrimary (citable) accession number: Q03L88
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 14, 2006
Last modified: July 22, 2015
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.