Reviewed, UniProtKB/Swiss-Prot Q03GJ0 (GUAC_PEDPA)

Names and origin

Protein names

Recommended name:
    GMP reductase
    EC=1.7.1.7
Alternative name(s):
    Guanosine 5'-monophosphate oxidoreductase
      Short name=Guanosine monophosphate reductase

Gene names

Name:	guaC
Ordered Locus Names:	PEPE_0595

Organism

Pediococcus pentosaceus (strain ATCC 25745 / 183-1w) [Complete proteome] [HAMAP]

Taxonomic identifier

278197 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Pediococcus

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides By similarity.
Catalytic activity	Inosine 5'-phosphate + NH(3) + NADP(+) = guanosine 5'-phosphate + NADPH.
Sequence similarities	Belongs to the IMPDH/GMPR family. GuaC type 2 subfamily.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: InterPro purine nucleotide metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	GMP reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	GMP reductase		PRO_0000294280
Regions
	Nucleotide binding	203 – 226	24	NADP Potential
Sites
	Active site	174	1	Thioimidate intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q03GJ0-1 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: 2C83AA2A6F2CBBAA	FASTA	325	36,061
	10 20 30 40 50 60 MKVFDYEDIQ LIPAKCIVRS RTECDPTVVL GEHRFKLPVV PANMQTIINE EIAEKLAEDG 70 80 90 100 110 120 YFYIMHRFEP ERRMDFVKKM KDKGLISSIS VGVKDDEYAL IDQLAEENLT PDYITIDVAH 130 140 150 160 170 180 GHAQSVIDMI HYIKEKLPAA FVIAGNVGTQ EGVRELENAG ADATKVGIGP GKVCITKIKT 190 200 210 220 230 240 GFGTGGWQLS ALRWCAKVAR KPLIADGGIR THGDIAKSIR FGATMVMIGS LFAGHIESPG 250 260 270 280 290 300 ETKVEDGVKY KEYFGSASQY QKGEAKNVEG KKIWIHQRGH LRDTLQAMRE DLQSSISYAG 310 320 GRDLEAIRKV DYVIVKNSIF NGDVL

References

[1]

"Comparative genomics of the lactic acid bacteria." Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V., Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V., Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M., Hawkins T. , Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A., Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W., Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y., Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R., O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T., Richardson P., Kozyavkin S., Weimer B.C., Mills D.A. Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006) [PubMed: 17030793] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000422 Genomic DNA. Translation: ABJ67682.1.
RefSeq	YP_804124.1.
3D structure databases
SMR	Q03GJ0. Positions 3-319.
ModBase	Search...
Genome annotation databases
GeneID	4418216.
GenomeReviews	Gene locus PEPE_0595 in contig CP000422_GR.
KEGG	ppe:PEPE_0595.
NMPDR	fig|278197.10.peg.547.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01511. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005994. GMP_reduct2. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DHase_GMPRtase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00478. IMPDH. 1 hit. [Graphical view]
PIRSF	PIRSF036500. GMP_red_Firmic. 1 hit.
TIGRFAMs	TIGR01306. GMP_reduct_2. 1 hit.
PROSITE	PS00487. IMP_DH_GMP_RED. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAC_PEDPA

Accession

Primary (citable) accession number: Q03GJ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	November 14, 2006
Last modified:	November 25, 2008
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)