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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Pediococcus pentosaceus (strain ATCC 25745 / CCUG 21536 / LMG 10740 / 183-1w)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei44S-adenosyl-L-methionineUniRule annotation1
Binding sitei69S-adenosyl-L-methionineUniRule annotation1
Binding sitei96S-adenosyl-L-methionineUniRule annotation1
Active sitei118By similarity1
Binding sitei118S-adenosyl-L-methionineUniRule annotation1
Binding sitei122SubstrateUniRule annotation1
Binding sitei154SubstrateUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:PEPE_0682
OrganismiPediococcus pentosaceus (strain ATCC 25745 / CCUG 21536 / LMG 10740 / 183-1w)
Taxonomic identifieri278197 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaePediococcus
Proteomesi
  • UP000000773 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002881951 – 212tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST212

Interactioni

Protein-protein interaction databases

STRINGi278197.PEPE_0682.

Structurei

3D structure databases

ProteinModelPortaliQ03GC9.
SMRiQ03GC9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni124 – 129Interaction with RNAUniRule annotation6
Regioni192 – 195Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZ1. Bacteria.
COG0220. LUCA.
HOGENOMiHOG000251689.
KOiK03439.
OMAiWITFPDP.
OrthoDBiPOG091H015P.

Family and domain databases

HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiView protein in InterPro
IPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
PANTHERiPTHR23417. PTHR23417. 1 hit.
PfamiView protein in Pfam
PF02390. Methyltransf_4. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiView protein in PROSITE
PS51625. SAM_MT_TRMB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q03GC9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVRNKPWAP ELIEAHPEKI VEKGQAFKGQ WNQRFEKEQP IFIEVGTGKG
60 70 80 90 100
QFIINMAKKY PQYNFIGIEI QKTVIAIALK NALEEEIPNL QFLYADGAEL
110 120 130 140 150
TDYFEDGEVS KVFLNFSDPW PKTRHEKRRL TFKTFLKVYE QILVKNGEVE
160 170 180 190 200
FKTDNQGLFE YSLYSLNNYG MTFEGVWLDL HNSEENEDNV ETEYEHKFSA
210
KGQPIYKLKA HF
Length:212
Mass (Da):24,942
Last modified:November 14, 2006 - v1
Checksum:i8F5EA41AD89E2580
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000422 Genomic DNA. Translation: ABJ67743.1.
RefSeqiWP_011673202.1. NC_008525.1.

Genome annotation databases

EnsemblBacteriaiABJ67743; ABJ67743; PEPE_0682.
GeneIDi33062656.
KEGGippe:PEPE_0682.

Similar proteinsi

Entry informationi

Entry nameiTRMB_PEDPA
AccessioniPrimary (citable) accession number: Q03GC9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: November 14, 2006
Last modified: September 27, 2017
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families