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Protein

Thymidylate synthase

Gene

thyA

Organism
Pediococcus pentosaceus (strain ATCC 25745 / CCUG 21536 / LMG 10740 / 183-1w)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei23dUMPUniRule annotation1
Active sitei198NucleophileUniRule annotation1
Binding sitei2215,10-methylenetetrahydrofolateUniRule annotation1
Binding sitei229dUMPUniRule annotation1
Binding sitei3155,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi178 – 179dUMP; shared with dimeric partnerUniRule annotation2
Nucleotide bindingi218 – 221dUMPUniRule annotation4
Nucleotide bindingi259 – 261dUMPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processNucleotide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
Ordered Locus Names:PEPE_1070
OrganismiPediococcus pentosaceus (strain ATCC 25745 / CCUG 21536 / LMG 10740 / 183-1w)
Taxonomic identifieri278197 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaePediococcus
Proteomesi
  • UP000000773 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000006471 – 316Thymidylate synthaseAdd BLAST316

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi278197.PEPE_1070.

Structurei

3D structure databases

ProteinModelPortaliQ03F96.
SMRiQ03F96.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0V. Bacteria.
COG0207. LUCA.
HOGENOMiHOG000257899.
KOiK00560.
OMAiKQYLDLC.
OrthoDBiPOG091H02L6.

Family and domain databases

CDDicd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact. 1 hit.
InterProiView protein in InterPro
IPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
PfamiView protein in Pfam
PF00303. Thymidylat_synt. 1 hit.
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiView protein in PROSITE
PS00091. THYMIDYLATE_SYNTHASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q03F96-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLEEQYLNLE RYVLENGHLK GDRTQTGTLS TFGYQMRFDL SEGFPLLTTK
60 70 80 90 100
RVPFGLIKSE LLWFLKGDTN IRYLLQHNNH IWDEWAFKKW VESDEYQGPD
110 120 130 140 150
MTDFGHRSLT DPEFNELYKI EKQRFTEQIL EDDTFSAKYG DLGNVYGSQW
160 170 180 190 200
RAWKTSTGET IDQISNVIDM IKNNPNSRRM IVSAWNPEDV PTSALPPCHS
210 220 230 240 250
LFQFYVADGK LSCQLYQRSG DIFLGIPFNI ASYALLTELI AKATGLEVGE
260 270 280 290 300
FIHTIGDAHI YSNHLDQVKE QLERTPRPAP KLKFKQVHDS IFDYEPGDIV
310
VEGYDPHPTI KAPVAV
Length:316
Mass (Da):36,392
Last modified:November 14, 2006 - v1
Checksum:i1C54D944C69C326A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000422 Genomic DNA. Translation: ABJ68126.1.
RefSeqiWP_011673474.1. NC_008525.1.

Genome annotation databases

EnsemblBacteriaiABJ68126; ABJ68126; PEPE_1070.
GeneIDi33062960.
KEGGippe:PEPE_1070.

Similar proteinsi

Entry informationi

Entry nameiTYSY_PEDPA
AccessioniPrimary (citable) accession number: Q03F96
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 14, 2006
Last modified: September 27, 2017
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families