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Q03F74 (PFKA_PEDPA) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-dependent 6-phosphofructokinase

Short name=ATP-PFK
Short name=Phosphofructokinase
EC=2.7.1.11
Alternative name(s):
Phosphohexokinase
Gene names
Name:pfkA
Ordered Locus Names:PEPE_1093
OrganismPediococcus pentosaceus (strain ATCC 25745 / 183-1w) [Complete proteome] [HAMAP]
Taxonomic identifier278197 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaePediococcus

Protein attributes

Sequence length322 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity. HAMAP-Rule MF_00339

Catalytic activity

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP-Rule MF_00339

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00339

Enzyme regulation

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate By similarity. HAMAP-Rule MF_00339

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP-Rule MF_00339

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00339

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00339.

Sequence similarities

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 322322ATP-dependent 6-phosphofructokinase HAMAP-Rule MF_00339
PRO_1000059783

Regions

Nucleotide binding72 – 732ATP By similarity
Nucleotide binding102 – 1054ATP By similarity
Region21 – 255Allosteric activator ADP binding; shared with dimeric partner By similarity
Region125 – 1273Substrate binding By similarity
Region169 – 1713Substrate binding By similarity
Region185 – 1873Allosteric activator ADP binding By similarity
Region213 – 2153Allosteric activator ADP binding By similarity
Region252 – 2554Substrate binding By similarity

Sites

Active site1271Proton acceptor By similarity
Metal binding1031Magnesium; catalytic By similarity
Binding site111ATP; via amide nitrogen By similarity
Binding site1541Allosteric activator ADP By similarity
Binding site1621Substrate; shared with dimeric partner By similarity
Binding site2221Substrate By similarity
Binding site2461Substrate; shared with dimeric partner By similarity

Sequences

Sequence LengthMass (Da)Tools
Q03F74 [UniParc].

Last modified November 14, 2006. Version 1.
Checksum: B6EFEE3AE5AF48C3

FASTA32234,248
        10         20         30         40         50         60 
MKRIGIMTSG GDAPGMNLAI RAVARKALSS GLEAYGINYG FAGLVAGDIH EFKAADLDDM 

        70         80         90        100        110        120 
VSQGGTMLYS ARYPEFAQEE SQLKGIEQLK KFGIDALVVI GGDGSYHGAL RLTEHGYNTI 

       130        140        150        160        170        180 
GLPGTIDNDI PFTDFTIGFD TALNTAVDAI DKIRDTAKSH QRVFAVQVMG RNAADIALWA 

       190        200        210        220        230        240 
GVASGADAVI APGFDYDVEA IANKLKKNRA NGKDYGIIVI AEGDANSDAA PEFIDQLKQY 

       250        260        270        280        290        300 
GDFDARATVI GHVQRGGVPS AKDRVLASKM GAYAVELLLE GKGGLAVGIL ENKVQAHNML 

       310        320 
DLFDAKHQAD DSLYQLSEDL SF 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000422 Genomic DNA. Translation: ABJ68148.1.
RefSeqYP_804590.1. NC_008525.1.

3D structure databases

ProteinModelPortalQ03F74.
SMRQ03F74. Positions 1-321.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING278197.PEPE_1093.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABJ68148; ABJ68148; PEPE_1093.
GeneID4417028.
KEGGppe:PEPE_1093.
PATRIC22875508. VBIPedPen34213_1038.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0205.
HOGENOMHOG000248870.
KOK00850.
OMAAKLERGH.
OrthoDBEOG644ZRM.

Enzyme and pathway databases

BioCycPPEN278197:GI4Y-1093-MONOMER.
UniPathwayUPA00109; UER00182.

Family and domain databases

HAMAPMF_00339. Phosphofructokinase_I_B1.
InterProIPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamPF00365. PFK. 1 hit.
[Graphical view]
PIRSFPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSPR00476. PHFRCTKINASE.
SUPFAMSSF53784. SSF53784. 1 hit.
TIGRFAMsTIGR02482. PFKA_ATP. 1 hit.
PROSITEPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePFKA_PEDPA
AccessionPrimary (citable) accession number: Q03F74
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 14, 2006
Last modified: July 9, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways