Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q03EY9

- SYI_PEDPA

UniProt

Q03EY9 - SYI_PEDPA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Pediococcus pentosaceus (strain ATCC 25745 / 183-1w)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei556 – 5561Aminoacyl-adenylateUniRule annotation
Binding sitei600 – 6001ATPUniRule annotation
Metal bindingi891 – 8911ZincUniRule annotation
Metal bindingi894 – 8941ZincUniRule annotation
Metal bindingi911 – 9111ZincUniRule annotation
Metal bindingi914 – 9141ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPPEN278197:GI4Y-1179-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:PEPE_1179
OrganismiPediococcus pentosaceus (strain ATCC 25745 / 183-1w)
Taxonomic identifieri278197 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaePediococcus
ProteomesiUP000000773: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 933933Isoleucine--tRNA ligasePRO_1000022096Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi278197.PEPE_1179.

Structurei

3D structure databases

ProteinModelPortaliQ03EY9.
SMRiQ03EY9. Positions 1-920.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi597 – 6015"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiLGRRSCQ.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03EY9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRVKDTLNLG KTKFKMRGNL PVNEQKREDI WAENKIYEQR QKLNEGKPSF
60 70 80 90 100
VLHDGPPYAN GNIHMGHAMN KISKDIIVRY KSMSGFRSPF VPGWDTHGLP
110 120 130 140 150
IEQQLTKEGY DRKKMSTADF RKLCHEYALK QVDKQRTDFK RLGISAEWDH
160 170 180 190 200
PYLTLNPEFE AQEIRTFGRM AELGLIYRGK KPVFWSWSSE SAMAEAEVEY
210 220 230 240 250
HDVTSPSAFY AEKVNDGKGV LDENTYFVVW TTTPWTIPAS EGITIDAGFD
260 270 280 290 300
YSVVQPAGED RKFVIATDRL NYAAEQFGWE DVQTLQVIRG RELDRIKAIH
310 320 330 340 350
PFDNDRELVV MLGDFVTLDS GTGLVHTAPG FGEDDFRVGK EYGLDIFVPV
360 370 380 390 400
DDRGYMTAEA GPDFEGVFYE DANAIALDKL KASNALLKQM DYVHSYPFDW
410 420 430 440 450
RTKKPIIFRA TPQWFASVDK IRDQILEAIE DVEFLPDWGQ KRLHNMIRDR
460 470 480 490 500
GDWVISRQRV WGVPLPIFYA EDGTPILEKE TINHVADLFE KNGSDIWFQW
510 520 530 540 550
EAKDLLPEGY SNEHSPNGEF TKENDIMDVW FDSGSSHQGV LAQRDYLEYP
560 570 580 590 600
ADLVLEGSDQ YRGWFNSSLI TSVAVSGKAP YKKVISQGFT LDGKGHKMSK
610 620 630 640 650
SLGNTIVPDE INKKMGAEII RLWVSSIDSS SDVRVSQENF VKASESYKKI
660 670 680 690 700
RNTVRYLLAN TSDFDPKENG VDYNELRPED RYMLVQFNEL ISKIRTSYDN
710 720 730 740 750
YDFIDIYKTL LNYIITDLSA FYLDFAKDVV YIEPENSLKR RSMQTVFYKI
760 770 780 790 800
LVGLTKLITP ILPHTAEEIW EYLREPEEFV QLAEMPEVAN YTDGADLLSK
810 820 830 840 850
WQSIKELRSH VLKALEEARD SKMIGKSMEA KATLYLDEST KQLVDTLGVD
860 870 880 890 900
LRLILIVSQL EVKALSEAPK DAAIFDDQLA VEITPAQGEV CERCRMTKTD
910 920 930
VGSDEHFATL CASCAQIVTE NYPEAITEGF EEK
Length:933
Mass (Da):106,724
Last modified:November 14, 2006 - v1
Checksum:i0FEB8CA603AF6363
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000422 Genomic DNA. Translation: ABJ68233.1.
RefSeqiYP_804675.1. NC_008525.1.

Genome annotation databases

EnsemblBacteriaiABJ68233; ABJ68233; PEPE_1179.
GeneIDi4417578.
KEGGippe:PEPE_1179.
PATRICi22875680. VBIPedPen34213_1123.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000422 Genomic DNA. Translation: ABJ68233.1 .
RefSeqi YP_804675.1. NC_008525.1.

3D structure databases

ProteinModelPortali Q03EY9.
SMRi Q03EY9. Positions 1-920.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 278197.PEPE_1179.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABJ68233 ; ABJ68233 ; PEPE_1179 .
GeneIDi 4417578.
KEGGi ppe:PEPE_1179.
PATRICi 22875680. VBIPedPen34213_1123.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi LGRRSCQ.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci PPEN278197:GI4Y-1179-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25745 / 183-1w.

Entry informationi

Entry nameiSYI_PEDPA
AccessioniPrimary (citable) accession number: Q03EY9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 14, 2006
Last modified: October 29, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3