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Q03EY9

- SYI_PEDPA

UniProt

Q03EY9 - SYI_PEDPA

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Protein
Isoleucine--tRNA ligase
Gene
ileS, PEPE_1179
Organism
Pediococcus pentosaceus (strain ATCC 25745 / 183-1w)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei556 – 5561Aminoacyl-adenylate By similarity
Binding sitei600 – 6001ATP By similarity
Metal bindingi891 – 8911Zinc By similarity
Metal bindingi894 – 8941Zinc By similarity
Metal bindingi911 – 9111Zinc By similarity
Metal bindingi914 – 9141Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPPEN278197:GI4Y-1179-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:PEPE_1179
OrganismiPediococcus pentosaceus (strain ATCC 25745 / 183-1w)
Taxonomic identifieri278197 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaePediococcus
ProteomesiUP000000773: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 933933Isoleucine--tRNA ligaseUniRule annotation
PRO_1000022096Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi278197.PEPE_1179.

Structurei

3D structure databases

ProteinModelPortaliQ03EY9.
SMRiQ03EY9. Positions 1-920.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotation
Add
BLAST
Motifi597 – 6015"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiLGRRSCQ.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03EY9-1 [UniParc]FASTAAdd to Basket

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MRVKDTLNLG KTKFKMRGNL PVNEQKREDI WAENKIYEQR QKLNEGKPSF    50
VLHDGPPYAN GNIHMGHAMN KISKDIIVRY KSMSGFRSPF VPGWDTHGLP 100
IEQQLTKEGY DRKKMSTADF RKLCHEYALK QVDKQRTDFK RLGISAEWDH 150
PYLTLNPEFE AQEIRTFGRM AELGLIYRGK KPVFWSWSSE SAMAEAEVEY 200
HDVTSPSAFY AEKVNDGKGV LDENTYFVVW TTTPWTIPAS EGITIDAGFD 250
YSVVQPAGED RKFVIATDRL NYAAEQFGWE DVQTLQVIRG RELDRIKAIH 300
PFDNDRELVV MLGDFVTLDS GTGLVHTAPG FGEDDFRVGK EYGLDIFVPV 350
DDRGYMTAEA GPDFEGVFYE DANAIALDKL KASNALLKQM DYVHSYPFDW 400
RTKKPIIFRA TPQWFASVDK IRDQILEAIE DVEFLPDWGQ KRLHNMIRDR 450
GDWVISRQRV WGVPLPIFYA EDGTPILEKE TINHVADLFE KNGSDIWFQW 500
EAKDLLPEGY SNEHSPNGEF TKENDIMDVW FDSGSSHQGV LAQRDYLEYP 550
ADLVLEGSDQ YRGWFNSSLI TSVAVSGKAP YKKVISQGFT LDGKGHKMSK 600
SLGNTIVPDE INKKMGAEII RLWVSSIDSS SDVRVSQENF VKASESYKKI 650
RNTVRYLLAN TSDFDPKENG VDYNELRPED RYMLVQFNEL ISKIRTSYDN 700
YDFIDIYKTL LNYIITDLSA FYLDFAKDVV YIEPENSLKR RSMQTVFYKI 750
LVGLTKLITP ILPHTAEEIW EYLREPEEFV QLAEMPEVAN YTDGADLLSK 800
WQSIKELRSH VLKALEEARD SKMIGKSMEA KATLYLDEST KQLVDTLGVD 850
LRLILIVSQL EVKALSEAPK DAAIFDDQLA VEITPAQGEV CERCRMTKTD 900
VGSDEHFATL CASCAQIVTE NYPEAITEGF EEK 933
Length:933
Mass (Da):106,724
Last modified:November 14, 2006 - v1
Checksum:i0FEB8CA603AF6363
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000422 Genomic DNA. Translation: ABJ68233.1.
RefSeqiYP_804675.1. NC_008525.1.

Genome annotation databases

EnsemblBacteriaiABJ68233; ABJ68233; PEPE_1179.
GeneIDi4417578.
KEGGippe:PEPE_1179.
PATRICi22875680. VBIPedPen34213_1123.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000422 Genomic DNA. Translation: ABJ68233.1 .
RefSeqi YP_804675.1. NC_008525.1.

3D structure databases

ProteinModelPortali Q03EY9.
SMRi Q03EY9. Positions 1-920.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 278197.PEPE_1179.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABJ68233 ; ABJ68233 ; PEPE_1179 .
GeneIDi 4417578.
KEGGi ppe:PEPE_1179.
PATRICi 22875680. VBIPedPen34213_1123.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi LGRRSCQ.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci PPEN278197:GI4Y-1179-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25745 / 183-1w.

Entry informationi

Entry nameiSYI_PEDPA
AccessioniPrimary (citable) accession number: Q03EY9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 14, 2006
Last modified: May 14, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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